Thomas F. Duchaine

TL;DR: It is shown here that translation initiation, specifically the 5′ cap recognition process, is repressed by endogenous let-7 miRNAs within the first 15 minutes of mRNA exposure to the extract when no destabilization of the transcript is observed.

TL;DR: It is demonstrated that miRNA-mediated mRNA deadenylation occurs subsequent to initial translational inhibition, indicating a two-step mechanism of miRNA action, which serves to consolidate repression.

TL;DR: This Review reflects on the molecular properties of the Dicer enzymes in small RNA silencing pathways, and rationalizes the newly discovered mutations on the basis of the activities and functions of its determinants.

TL;DR: It is shown here that human GW182 independently interacts with the PAN2–PAN3 and CCR4–NOT deadenylase complexes, and serves as both a platform that recruitsdeadenylases and as a deadenyase coactivator that facilitates the removal of the poly(A) tail by CCR 4–NOT.

TL;DR: It is shown that distinct RdRPs function sequentially to produce small RNAs that target endogenous loci in Caenorhabditis elegans and that ERGO-1 targets exhibit a nonrandom distribution in the genome and appear to include many gene duplications, suggesting that this pathway may control overexpression resulting from gene expansion.