Vito R T Zanotelli

TL;DR: It is shown that HRM outperformed shotgun proteomics both in the number of consistently identified peptides across multiple measurements and quantification of differentially abundant proteins, implying that DIA should be the preferred method for quantitative protein profiling.

TL;DR: In this paper, the authors used mass cytometry with extensive antibody panels to perform in-depth immune profiling of samples from 73 clear cell renal cell carcinoma (ccRCC) patients and five healthy controls.

TL;DR: A single-cell, spatially resolved analysis of breast cancer demonstrates the heterogeneity of tumour and stroma tissue and provides a more-detailed method of patient classification than the current histology-based system.

TL;DR: This work has developed an open-source, computational histology topography cytometry analysis toolbox (histoCAT) to enable interactive, quantitative, and comprehensive exploration of individual cell phenotypes, cell–cell interactions, microenvironments, and morphological structures within intact tissues.

TL;DR: In this paper, a systematic identification of meaningful metabolic enzyme regulation (SIMMER) was proposed, which can carry out regulatory inference at the level of single metabolic reactions by using cellular data.