Y
Yoshimi Nagai
Researcher at National Institute of Technology and Evaluation
Publications - 7
Citations - 4867
Yoshimi Nagai is an academic researcher from National Institute of Technology and Evaluation. The author has contributed to research in topics: Genome & Gene. The author has an hindex of 6, co-authored 7 publications receiving 4725 citations.
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Journal ArticleDOI
Whole genome sequencing of meticillin-resistant Staphylococcus aureus
Makoto Kuroda,Toshiko Ohta,Ikuo Uchiyama,Tadashi Baba,Harumi Yuzawa,Ichizo Kobayashi,Longzhu Cui,Akio Oguchi,Ken-ichi Aoki,Yoshimi Nagai,JianQi Lian,Teruyo Ito,Mutsumi Kanamori,Hiroyuki Matsumaru,Atsushi Maruyama,Hiroyuki Murakami,Akira Hosoyama,Yoko Mizutani-Ui,Noriko K. Takahashi,Toshihiko Sawano,Ryu-ichi Inoue,Chikara Kaito,Kazuhisa Sekimizu,Hideki Hirakawa,Satoru Kuhara,Susumu Goto,Junko Yabuzaki,Minoru Kanehisa,Atsushi Yamashita,Kenshiro Oshima,Keiko Furuya,Chie Yoshino,Tadayoshi Shiba,Masahira Hattori,Naotake Ogasawara,Hideo Hayashi,Keiichi Hiramatsu +36 more
TL;DR: Investigation of many newly identified gene products, including the 70 putative virulence factors, will greatly improve the understanding of the biology of staphylococci and the processes of infectious diseases caused by S aureus.
Journal ArticleDOI
Genome and virulence determinants of high virulence community-acquired MRSA.
Tadashi Baba,Fumihiko Takeuchi,Makoto Kuroda,Harumi Yuzawa,Ken-ichi Aoki,Akio Oguchi,Yoshimi Nagai,Natsuko Iwama,Kazuyuki Asano,Timothy S. Naimi,Hiroko Kuroda,Longzhu Cui,Kenji Yamamoto,Keiichi Hiramatsu +13 more
TL;DR: The whole genome sequence of MW2, a strain of community-acquired MRSA, was ascertained by shotgun cloning and sequencing and found that it carried a range of virulence and resistance genes that was distinct from those displayed on the chromosomes of extant S aureus strains.
Journal ArticleDOI
Complete Sequence and Gene Organization of the Genome of a Hyper-thermophilic Archaebacterium, Pyrococcus horikoshii OT3
Yutaka Kawarabayasi,Mituhiro Sawada,Hiroshi Horikawa,Yuji Haikawa,Yumi Hino,Saori Yamamoto,Mitsuo Sekine,Sin-ichi Baba,Hiroki Kosugi,Akira Hosoyama,Yoshimi Nagai,Mari Sakai,Keiko Ogura,Rie Otsuka,Hidekazu Nakazawa,Minako Takamiya,Yuhko Ohfuku,Tomomichi Funahashi,Toshihiro Tanaka,Yutaka Kudoh,Jun Yamazaki,Norihiro Kushida,Akio Oguchi,Ken-ichi Aoki,Takio Yoshizawa,Yoshinobu Nakamura,Frank T. Robb,Koki Horikoshi,Yaeko Masuchi,Hiroaki Shizuya,Hisasi Kikuchi +30 more
TL;DR: The complete sequence of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3, has been determined by assembling the sequences of the physical map-based contigs of fosmid clones and of long polymerase chain reaction (PCR) products which were used for gap-filling.
Journal ArticleDOI
Complete genome sequence of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1.
Yutaka Kawarabayasi,Yumi Hino,Hirosh Horikawa,Syuji Yamazaki,Yuji Haikawa,Koji Jin-no,Mikio Takahashi,Mitsuo Sekine,Sin-ichi Baba,Akiho Ankai,Hiroki Kosugi,Akira Hosoyama,Shigehiro Fukui,Yoshimi Nagai,Keiko Nishijima,Hidekazu Nakazawa,Minako Takamiya,Sayaka Masuda,Tomomichi Funahashi,Toshihiro Tanaka,Yutaka Kudoh,Jun Yamazaki,Norihiro Kushida,Akio Oguchi,Ken-ichi Aoki,Kenji Kubota,Yoshinobu Nakamura,Norimichi Nomura,Yoshihiko Sako,Hisasi Kikuchi +29 more
TL;DR: The complete sequence of the genome of an aerobic hyper-thermophilic crenarchaeon, Aeropyrum pernix K1, which optimally grows at 95 degrees C, has been determined by the whole genome shotgun method with some modifications.
Journal ArticleDOI
Complete genome sequence of an aerobic thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain7.
Yutaka Kawarabayasi,Yumi Hino,Hiroshi Horikawa,Koji Jin-no,Mikio Takahashi,Mitsuo Sekine,Sin-ichi Baba,Akiho Ankai,Hiroki Kosugi,Akira Hosoyama,Shigehiro Fukui,Yoshimi Nagai,Keiko Nishijima,Rie Otsuka,Hidekazu Nakazawa,Minako Takamiya,Yumiko Kato,Takio Yoshizawa,Toshihiro Tanaka,Yutaka Kudoh,Jun Yamazaki,Norihiro Kushida,Akio Oguchi,Ken-ichi Aoki,Sayaka Masuda,Masao Yanagii,Masami Nishimura,Akihiko Yamagishi,Tairo Oshima,Hisasi Kikuchi +29 more
TL;DR: Sequence comparison provided evidence suggesting the integration of plasmid, rearrangement of genomic structure, and duplication of genomic regions that may be responsible for the larger genomic size of the S. tokodaii strain7 genome.