Yukio Nakamura

TL;DR: This work integrates multiple transcript collections to generate a comprehensive atlas of 27,919 human lncRNA genes with high-confidence 5′ ends and expression profiles across 1,829 samples from the major human primary cell types and tissues, identifying 19,175 potentially functional lncRNAs in the human genome.

TL;DR: A pooled clustered regularly interspaced palindromic repeat-Cas9 guide RNA libraries are developed to perform in situ saturating mutagenesis of the human and mouse enhancers and reveal critical minimal features and discrete vulnerabilities of these enhancers.

TL;DR: This is the first demonstration of the feasibility of using immortalized human erythroid progenitor cell lines as an ex vivo source for production of enucleated RBCs, and these immortalized cell lines all produce enucleations after induction of differentiation in vitro.

TL;DR: Here, CRISPR–Cas9-mediated genome editing of human blood progenitors to mutate a 13-nt sequence that is present in the promoters of the HBG1 and HBG2 genes, thereby recapitulating a naturally occurring HPFH-associated mutation are performed.

TL;DR: It is found that the LRF/ZBTB7A transcription factor occupies fetal γ-globin genes and maintains the nucleosome density necessary for γ -globin gene silencing in adults, and that LRF confers its repressive activity through a NuRD repressor complex independent of the fetal globin repressor BCL11A.