Graduate University of Advanced Technology

About: Graduate University of Advanced Technology is a education organization based out in Kerman, Iran. It is known for research contribution in the topics: Carbon paste electrode & Electrochemical gas sensor. The organization has 890 authors who have published 2169 publications receiving 31027 citations.

Enhancing cadmium removal by low-cost nanocomposite adsorbents from aqueous solutions; a continuous system

Abstract: Removal of cadmium ions from an aquatic solution was investigated by low-cost adsorbents and compared when modifying them by Fe-S nanoparticles. Nanocomposites were synthesized by a new experimental method under simple and inexpensive procedure by Fe-S nanoparticles, which possessed good characteristics of the surface area and magnetic property. These nanocomposite adsorbents removed cadmium at larger percentages than pure adsorbents. Iron filings had only a minor effect on the removal of cadmium, but when they were modified with Fe-S nanoparticles, the adsorption rate reached over 98.5%. Under operating conditions of pH 7, a concentration of 3 ppm, and a flow rate of 5 ml/min, the highest cadmium adsorption in water was caused by the iron nanocomposite.

Expression of the truncated tissue plasminogen activator ( K2S ) gene in tobacco chloroplast

Abstract: As because the plant plastid genome is highly polyploid, the transformation of chloroplasts permits the introduction of thousands of copies of foreign genes per plant cell and generates extraordinarily high levels of recombinant protein. Human tissue-type plasminogen activator is one of the most important pharmaceutical proteins involved in the breakdown of blood clots in brain and heart blood vessels. We report the introduction and expression of the truncated human tissue plasminogen activator (K2S) gene in tobacco chloroplasts. The K2S-containing vector pKCZK2S was successfully transferred to tobacco plastomes using the biolistic delivery procedure. Transplastomic plants were selected on RMOP medium containing spectinomycin (500 mg/l). In order to achieve homoplasmy, several rounds of selection and regeneration were performed. The presence, site-specific integration, homoplasmy, expression and activity assay of the transgene were confirmed in the transplastomic plants by PCR, Southern-blot, RT-PCR, SDS-PAGE, ELISA, Dot-blot, Western-blot and zymography analysis. Our results show that the tissue plasminogen activator (K2S form) protein to be expressed in tobacco chloroplasts in active form.