Showing papers by "Kyoto University published in 1975"

Thyroplasty type I (lateral compression) for dysphonia due to vocal cord paralysis or atrophy.

Abstract: Based on the experimental results of thyroplasty, thyroplasty type I which aims at medical shifting the vocal cord was performed on 8 patients with dysphonia, 6 with vocal cord paralysis and 2 with vocal cord atrophy. The surgery was conducted on either in- or out-patient basis and local anesthesia was used. Usually, a rectangular incision was made on the thyroid cartilage at the level of the vocal cord, and the fragmemt was depressed inward. A cartilage piece taken from the opposite side was used as a wedge, if necessary, to enhance the effect of lateral compression of the vocal cord. The voice after surgery was generally satisfactory, except in one case of traumatic vocal cord paralysis. Complications such as stridor or dyspnea were nil. As surgical intervention inside the thyroid cartilage is minimal, fine and reliable adjustment of depression is possible during the surgery.

Localization of masticatory motoneurons in the cat and rat by means of retrograde axonal transport of horseradish peroxidase

Abstract: Topographical localization of monotoneurons supplying the masticatory muscles was investigated in the cat and rat, utilizing retrograde axonal transport of horseradish peroxidase. Following injection of horseradish peroxidase in each masticatory muscle, motoneurons labelled with peroxidase were seen to be aggregated into a cluster within the motor nucleus of the trigeminal nerve. Such clusters of peroxidase-motoneurons innervating each masticatory muscle were demarcated more sharply in kittens than in adult animals. The pattern of the nuclear representation of the masticatory muscles was found to be essentially the same in the cat and rat; it could be summarized as follows: The motor nucleus of the trigeminal nerve could be divided cytoarchitectonically into the dorsolateral and ventromedial divisions; the former was seen in almost the whole rostrocaudal extent of the nucleus, while the latter was localized at the levels of caudal two thirds of the nucleus. In the dorsolateral division, the temporal muscle was represented dorsally and dorsomedially, the masseter muscle ventrolaterally, and the pterygoid muscles ventromedially. In the ventromedial division, the anterior digastric muscle was represented dorsomedially, and the mylohyoid muscle ventrolaterally. It was also confirmed that the motoneurons supplying the posterior digastric muscle were localized in the accessory facial nucleus.

Acetyl-coenzyme-A carboxylase from rat liver. Subunit structure and proteolytic modification.

Abstract: The subunit structure of rat liver acetyl-coenzyme-A carboxylase has been studied by polyacrylamide gel electrophoresis in the presence of dodecylsulfate. A number of individual preparations of the enzyme purified by the same procedures exhibited three different types of electrophoretic patterns as follows: first, a single slow-moving protein band (Mr 230000); secondly, two adjacent fast-moving protein bands (Mr 124000 and 118 000); finally, all three protein bands. With the use of the [14C]biotin-labelled enzyme, the biotinyl prosthetic group was shown to be associated with the polypeptide of 230 000 Mr as well as with that of 124 000 Mr, but not with the polypeptide of 118 000 Mr. Studies were next made with the labelled enzyme to examine the possibility that the two light polypeptides might have been formed by proteolytic modification of the heavy polypeptide during the procedures used for the purification of the enzyme. Treatment of the enzyme with trypsin or chymotrypsin resulted in cleavage of the heavy polypeptide into two nonidentical polypeptides with molecular weights of approximately 120 000. Incubation of the enzyme with proteases derived from rat liver converted the heavy polypeptide into lighter polypeptides of 80 000–130 000 Mr. Acetyl- CoA carboxylase isolated from crude rat liver extracts by means of immunoprecipitation with specific antibody invariably showed only the heavy polypeptide. The biotin content of the enzyme was found to be 1 mol per 237 000 g protein. These results indicate that rat liver acetyl-CoA carboxylase, unlike bacterial and plant biotin enzymes, has only one kind of subunit, which has a molecular weight of 230 000 and contains one molecule of biotin. Thus, the mammalian enzyme exhibits a highly integrated subunit structure.

Studies on ectopic ACTH-producing tumors. II. Clinical and biochemical features of 30 cases

Abstract: This report describes the clinical and biochemical features of 30 cases of ectopic ACTH-producing tumors diagnosed by the detection of ACTH in the tumor tissues. Several uncommon tumors, such as tumors of the esophagus, stomach, and larynx, were included in this series. None of the patients with bronchogenic carcinoma showed signs of classical Cushing's syndrome, whereas 7 of the remaining 13 patients with other tumors were Cushingoid in appearance. Adrenocortical hyperfunction was present in 61 percent at the first examination and developed during the course of the disease in 18 percent more. In the remaining patients (21 percent), adrenocortical function remained within normal limits. These results indicate that there exist ectopic ACTH-producing tumors without clinical and biochemical sequelae of excess hormone. In some of the tumor extracts studied, MSH and CRF-like activities and serotonin were detected. This suggests that multiple hormone production is not uncommon in ectopic ACTH-producing tumors.

Fine-structure turbulence in the wall region of a turbulent boundary layer

Abstract: Measurements have been made concerning the fine structure of the turbulence in the part adjacent to the wall of the wall region of a plane turbulent boundary layer. The objective was to gain further information concerning the larger-scale disturbance mechanism which is mainly responsible for the generation of turbulence. Hot-wire anemomet.ry was used and information on the fine structure was obtained by differentiating and filtering the hot-wire signal.The distributions of the Kolmogorov microscale and of the flatness and skewness factors of the axial fluctuating velocity u and its first and second derivative determined at two Reynolds numbers suggest the existence of Reynolds number similarity. In the region y+ 100) the flatness and skewness factors approach values obtained in shear-free turbulence at the same turbulence Reynolds number.The fine structure of the turbulence is strongly associated with and dominated by the random, larger-scale, intermittent inrush-ejection cycle. In the viscous sublayer both the fine structure, and the large-scale mechanism of the turbulence are influenced mainly by the inrush phase, while further out in the wall region (y+ > 40) they are influenced by both inrush and ejection. As a result, in the viscous sublayer the average burst periods of the high frequency turbulence components and their flatness factors (of ∂u/∂t and of ∂2u/∂t2) attain values twice those in the outer part.The change in the mechanism of the fine structure with distance from the wall is clearly demonstrated by the spectra of non-negative variables, i.e. (∂u/∂t)2 and (∂2u/∂t2)2. The spectra agree with each other and decrease with increasing frequency, following a power law as predicted by Gurvich & Yaglom (1967). The power law applies to almost the whole frequency range, when the highest, viscous, frequency range is excluded. However, the exponent is different for the viscous sublayer and the outer part of the wall region. In the buffer layer the spectra have two distinct power-law regions. In the lower frequency range the exponent is the same as that for the viscous sublayer, while in the higher frequency range it is the same as that for the outer part of the wall region.

Turbulence of open channel flow over smooth and rough beds

Abstract: In the last decade a broad knowledge of dynamics of wall turbulence in water flow has been obtained through development of a hot-film anemometer or a hydrogen-bubble method. One of the headmost studies in this field was done by a group of Stanford University1) under the direction of Kline who looked for the mechanism of turbulence production by means of flow visualization with the hydrogen-bubble method. Several experimental works done by use of both flow visualization and point measurements have recently brought to light turbulence characteristics of the shear flow, with aid of improved methods of data analysis. Turbulence measurments of open-channel shear flow over a smooth or rough bed were vigorously done by Raichlen2), McQuivey et al.3), Ishii et a1.4) and Imamoto5) by making use of singlesensor hot-film anemometer. It is very interesting in practice to investigate how the structure of turbulence would be influenced by hydraulic parameters such as Reynolds

Microbody of Methanol-Grown Yeasts

Abstract: Profuse appearance of microbodies was observed in the cells of methanol-utilizing yeasts in connection with the enhanced catalase activity. These microbodies were isolated successfully by means of sucrose gradient centrifugation from the methanol-grown cells of Kloeckera sp. no. 2201. Localization of a flavin-dependent alcohol oxidase as well as characteristic microbody enzymes (catalase and d-amino acid oxidase) were ascertained in the isolated microbodies, whereas formaldehyde and formate dehydrogenases were detected in the cytoplasmic region. Localization of catalase in the isolated microbody was also demonstrated by the cytochemical technique with 3,3′-diaminobenzidine.

Ionized cluster beam deposition

Abstract: We describe a form of deposition in which material is vaporized in a crucible and the vapor then ejected through a fine nozzle at the focus of an electron beam in a high vacuum. The vapor, on emerging from the nozzle, is partially condensed into clusters that are ionized by electron bombardment and then accelerated onto the substrate. The deposited films show good adhesion and large crystallite size. Examples include the deposition of Cu onto glass, Si onto Si, and ZnS onto NaCl.

Electrophysiological studies of the projections from the parietal association area to the cerebellar cortex

Abstract: 1. Responses evoked in the cerebellar cortex by stimulation of the parietal association cortex (rostral portions of the middle suprasylvian gyrus) were recorded and analysed in cats, and were compared with those by stimulation of the motor cortex (anterior sigmoid gyrus). 2. The parietal stimulation elicited early mossy fibre and late climbing fibre responses in the cerebellar cortex. The mossy fibre responses appeared at a latency of 2.0--2.5 msec and predominantly in the lateral (hemispherical) part of the contralateral cerebellum (mainly crus I, crus II and paramedian lobules). Cutting of the inferior cerebellar peduncle produced little or no influence upon the mossy fibre responses, which suggests that the mossy fibre responses are mediated chiefly by the pontine nuclei. 3. The climbing fibre responses were recorded at a latency of 17--19 msec and markedly in the contralateral intermediate and medial parts of IV--VI lobules. The responses were easily sppressed by anaesthesia and depended on the conditions of experimental animals. The unstable appearance of the responses and their longer latencies than those of the climbing fibre responses due to stimulation of the motor cortex imply indirect pathways from the parietal association cortex to the inferior olive. 4. The predominant projection of the parietal-induced mossy fibre responses to the lateral part of the cerebellum was compared with the mossy fibre projection from the motor cortex and was discussed as an important component in the cerebrocerebellar loops.

Excitation and Detection of Coherence between Forbidden Levels in Three-Level Spin System by Multi-Step Processes

Abstract: The coherent superposition of the states between which magnetic dipole transition is forbidden has been excited and detected by means of a multi-step application of resonant rf pulses in a three-level NMR system. The experiment was carried out on 27 Al nuclei in Al 2 O 3 where the quantization axes of the quadrupole and the magnetic coupling were parallel. Free decay and echo signals associated with the coherence were observed, and transfer of coherence between nonresonant pairs of levels was studied in detail.

Nonlinear Interaction between Short and Long Capillary-Gravity Waves

Abstract: Coupled nonlinear partial differential equations, which describe a nonlinear interaction between short and long capillary-gravity waves on a liquid layer of uniform depth, are derived by the derivative expansion method. The short and the long waves can exchange energy in a resonant manner, if the group velocity of the short wave is close to the phase velocity of the long wave. It is found that the long wave can take a form of rarefactive (convex downwards) solitary wave due to the resonant interaction. This should be compared with the well-known gravity wave soliton which is compressive (convex upwards) in the absence of the short wave.

Existence of a β-ionone ring-binding site in the rhodopsin molecule

Abstract: RHODOPSIN is a carotenoid-protein, in which the 11-cis retinal chromophore, is coupled to the colourless protein, opsin. The linkage between them is a protonated Schiff base between the aldehyde group of 11-cis retinal and an e-amino group of L-lysine in opsin1–4.

Development of microbodies in candida tropicalis during incubation in a n-alkane medium.

Abstract: Development of microbodies in Candida tropicalis pK 233 was studied mainly by electron microscopical observation. The yeast cells, precultured on malt extract, scarcely contained microbodies and showed very low catalase activity. When the precultured cells were transferred to a n-alkane medium and incubated with shaking, the number of microbodies increased and concomitantly the activity of catalase was enhanced. That is, both the area ratio of microbodies in the cell and the ratio of microbodies to cytoplasm in area increased significantly during the utilization of n-alkanes for 8 hrs. Localization of catalase in the microbodies was demonstrated cytochemically by use of 3,3′-diaminobenzidine, but other organella in the cell, except for vacuoles appearing in the early growth phase and mitochondria, were not stained with this reagent. Microbodies seemed to grow by division. Biogenesis of microbodies in the yeast cells is also discussed.

Purification of Bisphosphoglyceromutase, 2, 3‐Bisphosphoglycerate Phosphatase and Phosphoglyceromutase from Human Erythrocytes

Abstract: Bisphosphoglyceromutase, 2,3-bisphosphoglycerate phosphatase and phosphoglyceromutase have been purified from human red cells. Three enzymes were co-purified throughout all purification steps. Three fractions (peaks I, II and III) which were chromatographically separable and had three activities in different ratios were obtained. Peak III which contained the main bisphosphoglyceromutase and 2,3-bisphosphoglycerate phosphatase activities was purified to homogeneity by electrophoretic and ultracentrifugal analyses. The homogeneous preparation had the phosphoglyceromutase activity. The three activities were lost at the same rate during thermal inactivation. Thus, bisphosphoglyceromutase and 2,3-bisphosphoglycerate phosphatase activities, which are responsible for 2,3-bisphosphoglycerate metabolism in red cells, are displayed by the same enzyme protein which has phosphoglyceromutase activity. Peaks I and II were rich in the phosphoglyceromutase activity. Both peaks showed bisphosphoglyceromutase and 2,3-bisphosphoglycerate phosphatase activities, although these two activities were much smaller than those of peak III. Some of the enzymic properties of peak III are described. Comparative studies on three peaks showed that the phosphoglyceromutase of peak III differed from that of peaks I and II in the kinetic property and thermostability.