Northern Illinois University

About: Northern Illinois University is a education organization based out in DeKalb, Illinois, United States. It is known for research contribution in the topics: Large Hadron Collider & Population. The organization has 8818 authors who have published 20008 publications receiving 632341 citations. The organization is also known as: NIU.

An efficient method for the isolation and purification of oligoribonucleotides

Abstract: Problems associated with the use of tetrabutylammonium fluoride like incomplete desilylation and removal of the tetrabutylammonium salts during large scale syntheses of oligoribonucleotides (RNA) have been eliminated by the use of triethylamine trihydrofluoride and precipitation of the RNA with 1-butanol. An efficient anion-exchange HPLC method has been developed for the purification of chemically synthesized RNA and the resulting product precipitated directly by the addition of 1-propanol. A new activator, 5-ethylthio-1H-tetrazole significantly enhances the synthesis quality and yield of oligoribonucleotides. RNA synthesized using these improvements has been shown to be biologically active by a comparative ribozyme-substrate assay.

The turnaround of the London ambulance service computer-aided despatch system (LASCAD)

Abstract: The implementation of the Computer-Aided Despatch system at the London Ambulance Service has been one of the most notorious cases of failure within the information systems (IS) literature. What is less well known is that there followed, some time later, a much more successful implementation, described as a turnaround. This paper, based on a case study approach, describes the context and detail of that implementation. A framework from the literature, used in an analysis of the initial failure, is used to analyse and compare the similarities and differences in the development of the two systems. The framework provides four interacting elements and relationships for analysis. These elements are Supporters, Project Organisation, Information System, and the Environment in which they operate. The turnaround system was found to address directly almost all the issues identified as problematic in the failure. These included the approach taken by management to understand the needs of users, including issues unrelated to the system itself, their involvement in the development process, an improvement in the availability of resources (brought about in some part because of the previous failure), the ability to follow a relaxed timeline driven by users' acceptance levels, the preparation of infrastructure projects to develop confidence, participation and prototyping, thorough testing, phased and simple implementation, and trust building. Certain environmental factors could not be so directly addressed but nevertheless were overcome by attention to detail and internal needs. Conclusions indicate that the factors addressed are not new and are to be found in the success literature. What is unusual is that they were implemented in this case in such unlikely circumstances.

The social phobia and social interaction anxiety scales: Evaluation of psychometric properties

Abstract: The present investigations examined the factor structure and psychometric properties of two new self-report measures of social phobia, the Social Interaction Anxiety Scale (SIAS) and the Social Phobia Scale (SPS). A confirmatory factor analysis in Study I provided support for the fit of a two-factor model of the SIAS and SPS. Internal consistency estimates were high for the original two scales with a sample of 200 undergraduates. Also, using an item parceling procedure, the obtained internal consistency reliability indices for each parcel were acceptable. Results of the CFA in Study II provided support for the factorial stability of the model identified in Study I. Furthermore, multisample analyses showed invariant patterns for factor loadings and factor correlations across 138 men and 272 women. Gender differences were not observed in the mean SIAS and SPS scale and item scores. Both scales correlated negatively and significantly with measures of social desirability. Concurrent validity was established for the scales. The SPS was less specific than the SIAS to symptoms of social phobia.