Sultan Qaboos University

About: Sultan Qaboos University is a education organization based out in Muscat, Oman. It is known for research contribution in the topics: Population & Medicine. The organization has 7767 authors who have published 14605 publications receiving 241390 citations. The organization is also known as: SQU.

Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations.

Abstract: Metabolic engineering (ME) of Clostridium acetobutylicum has led to increased solvent (butanol, acet- one, and ethanol) production and solvent tolerance, thus demonstrating that further efforts have the potential to create strains of industrial importance. With recently devel- oped ME tools, it is now possible to combine genetic modifications and thus implement more advanced ME strategies. We have previously shown that antisense RNA (asRNA)-based downregulation of CoA transferase (CoAT, the first enzyme in the acetone-formation pathway) results in increased butanol to acetone selectivity, but overall reduced butanol yields and titers. In this study the alco- hol/aldehyde dehydrogenase (aad) gene (encoding the bifunctional protein AAD responsible for butanol and etha- nol production from butyryl-CoA and acetyl-CoA, respec- tively) was expressed from the phosphotransbutyrylase (ptb) promoter to enhance butanol formation and selectivity, while CoAT downregulation was used to minimize acetone production. This led to early production of high alcohol (butanol plus ethanol) titers, overall solvent titers of 30 g/L, and a higher alcohol/acetone ratio. Metabolic flux analysis revealed the likely depletion of butyryl-CoA. In order to increase then the flux towards butyryl-CoA, we examined the impact of thiolase (THL, thl) overexpression. THL converts acetyl-CoA to acetoacetyl-CoA, the first step of the pathway from acetyl-CoA to butyryl-CoA, and thus, combining thl overexpression with aad overexpression decreased, as expected, acetate and ethanol production while increasing acetone and butyrate formation. thl overexpres- sion in strains with asRNA CoAT downregulation did not significantly alter product formation thus suggesting that a more complex metabolic engineering strategy is necessary to enhance the intracellular butyryl-CoA pool and reduce the acetyl-CoA pool in order to achieve improved butanol titers and selectivity. Biotechnol. Bioeng. 2009;102: 38-49.

Twelve tips for rapidly migrating to online learning during the COVID-19 pandemic

Abstract: The COVID-19 pandemic has resulted in a massive adaptation in health professions education, with a shift from in-person learning activities to a sudden heavy reliance on internet-mediated education. Some health professions schools will have already had considerable educational technology and cultural infrastructure in place, making such a shift more of a different emphasis in provision. For others, this shift will have been a considerable dislocation for both educators and learners in the provision of education. To aid educators make this shift effectively, this 12 Tips article presents a compendium of key principles and practical recommendations that apply to the modalities that make up online learning. The emphasis is on design features that can be rapidly implemented and optimised for the current pandemic. Where applicable, we have pointed out how these short-term shifts can also be beneficial for the long-term integration of educational technology into the organisations' infrastructure. The need for adaptability on the part of educators and learners is an important over-arching theme. By demonstrating these core values of the health professions school in a time of crisis, the manner in which the shift to online learning is carried out sends its own important message to novice health professionals who are in the process of developing their professional identities as learners and as clinicians.

Instructors' Acceptance of Learning Management Systems: A Theoretical Framework

Abstract: In the knowledge age, the use of the information technology (IT) tools including learning management systems (LMS) has became an imperative. The adoption rate of LMS in academic and training institutions is very promising worldwide. Learning Management system includes several tools that provide academic and training institutions efficient and effective means to support distance education and supplement their traditional way of teaching. LMS also provide academic insinuations mechanisms and tools to store, manage, and share its academic resources and knowledge. Instructors' acceptance is essential for the deployment of LMS. The success of LMS in any institution starts by instructors' acceptance, which in turns initiates and promotes learners' utilization of LMS. Consequently, the objective of this paper is to develop a theoretical framework for evaluating instructors' acceptance of LMS based on the Technology Acceptance Model. This framework provides a comprehensive look of the critical factors that influence the instructors' perceived ease of use and perceived usefulness of LMS and consequently the actual use. These critical factors are related to the instructor, organization, and technology. Instructor factors include self efficacy, attitude toward LMS, experience, teaching style and personal innovativeness. Organization factors include motivators, technology alignment, organization support, technical support and training. Technology factors include system quality, information quality and service quality.

Identification techniques for highly boosted W bosons that decay into hadrons

Abstract: In searches for new physics in the energy regime of the LHC, it is becoming increasingly important to distinguish single-jet objects that originate from the merging of the decay products of W bosons produced with high transverse momenta from jets initiated by single partons. Algorithms are defined to identify such W jets for different signals of interest, using techniques that are also applicable to other decays of bosons to hadrons that result in a single jet, such as those from highly boosted Z and Higgs bosons. The efficiency for tagging W jets is measured in data collected with the CMS detector at a center-of-mass energy of 8 TeV, corresponding to an integrated luminosity of 19.7 inverse femtobarns. The performance of W tagging in data is compared with predictions from several Monte Carlo simulators.