University of Avignon

About: University of Avignon is a education organization based out in Avignon, Provence-Alpes-Côte-d'Azur, France. It is known for research contribution in the topics: Population & Speaker recognition. The organization has 1526 authors who have published 3766 publications receiving 88928 citations.

A new simulation for modelling the topology of earthworm burrow systems and their effects on macropore flow in experimental soils

Abstract: Earthworm burrows (i.e. macropores) are organised in burrow systems with various geometric properties. These burrow systems have a significant effect on soil processes, particularly the movement of water in the soil. But the relationships between earthworm burrow systems and their hydraulic properties are not well established because experimental studies of burrow geometry are difficult to perform. Although X-ray computed tomography has revolutionised the 3D description of burrow systems, this method is both time consuming and expensive. This paper presents a new, cheap and rapid approach. A computer model simulating the burrowing behaviour of earthworms was developed from experimental studies. A saturated flow model was then superimposed on the structure of the simulated burrow system to explore the relationships between the burrow systems and their hydraulic properties. Simulations of individual burrow systems were obtained for two different species of earthworm belonging to different ecological groups. Structural parameters of burrow systems (pore space, interconnectedness, connectivity and the number of openings at the limits of the space) were calculated and linked with estimates of permeability using regressions. In our results connectivity gave the best prediction of the difference in permeability between the two sets of burrow systems determined by the burrowing behaviour of the earthworms. Pore space, interconnectedness and the number of burrow openings explained fewer variations.

A class of mild surfactants that keep integral membrane proteins water-soluble for functional studies and crystallization

Abstract: Mixed protein-surfactant micelles are used for in vitro studies and 3D crystallization when solutions of pure, monodisperse integral membrane proteins are required. However, many membrane proteins undergo inactivation when transferred from the biomembrane into micelles of conventional surfactants with alkyl chains as hydrophobic moieties. Here we describe the development of surfactants with rigid, saturated or aromatic hydrocarbon groups as hydrophobic parts. Their stabilizing properties are demonstrated with three different integral membrane proteins. The temperature at which 50% of the binding sites for specific ligands are lost is used as a measure of stability and dodecyl-β-D-maltoside ('C12-b-M') as a reference for conventional surfactants. One surfactant increased the stability of two different G protein-coupled receptors and the human Patched protein receptor by approximately 10°C compared to C12-b-M. Another surfactant yielded the highest stabilization of the human Patched protein receptor compared to C12-b-M (13°C) but was inferior for the G protein-coupled receptors. In addition, one of the surfactants was successfully used to stabilize and crystallize the cytochrome b(6 )f complex from Chlamydomonas reinhardtii. The structure was solved to the same resolution as previously reported in C12-b-M.

Characterization of procyanidin B2 oxidation products in an apple juice model solution and confirmation of their presence in apple juice by high-performance liquid chromatography coupled to electrospray ion trap mass spectrometry

Abstract: Procyanidins (i.e. condensed tannins) are polyphenols commonly found in fruits. During juice and cider making, apple polyphenol oxidase catalyzes the oxidation of caffeoylquinic acid (CQA) into its corresponding o-quinone which further reacts with procyanidins and other polyphenols, leading to the formation of numerous oxidation products. However, the structure and the reaction pathways of these neoformed phenolic compounds are still largely unknown. Experiments were carried out on a model system to gain insights into the chemical processes occurring during the initial steps of fruit processing. Procyanidin B2 was oxidized by caffeoylquinic acid o-quinone (CQAoq) in an apple juice model solution. The reaction products were monitored using high performance liquid chromatography (HPLC) coupled to ultraviolet (UV)-visible and electrospray tandem mass spectrometry (ESI-MS/MS) in the negative mode. Oxidative conversion of procyanidin B2 ([M-H](-) at m/z 577) into procyanidin A2 at m/z 575 was unambiguously confirmed. In addition, several classes of products were characterized by their deprotonated molecules ([M-H](-)) and their MS/MS fragmentation patterns: hetero-dimers (m/z 929) and homo-dimers (m/z 1153 and 705) resulting from dimerization involving procyanidin and CQA molecules; intramolecular addition products at m/z 575, 573, 927, 1151 and 703. Interestingly, no extensive polymerization was observed. Analysis of a cider apple juice enabled comparison with the results obtained on a biosynthetic model solution. However, procyanidin A2 did not accumulate but seemed to be an intermediate in the formation of an end-product at m/z 573 for which two structural hypotheses are given. These structural modifications of native polyphenols as a consequence of oxidation probably have an impact on the organoleptic and nutritional properties of apple juices and other apple-derived foods.

Modeling soil evaporation efficiency in a range of soil and atmospheric conditions using a meta‐analysis approach

Abstract: A meta-analysis data-driven approach is developed to represent the soil evaporative efficiency (SEE) defined as the ratio of actual to potential soil evaporation. The new model is tested across a bare soil database composed of more than 30 sites around the world, a clay fraction range of 0.02-0.56, a sand fraction range of 0.05-0.92, and about 30,000 acquisition times. SEE is modeled using a soil resistance ($r_{ss}$) formulation based on surface soil moisture ($\theta$) and two resistance parameters $r_{ss,ref}$ and $\theta_{efolding}$. The data-driven approach aims to express both parameters as a function of observable data including meteorological forcing, cut-off soil moisture value $\theta_{1/2}$ at which SEE=0.5, and first derivative of SEE at $\theta_{1/2}$, named $\Delta\theta_{1/2}^{-1}$. An analytical relationship between $(r_{ss,ref};\theta_{efolding})$ and $(\theta_{1/2};\Delta\theta_{1/2}^{-1})$ is first built by running a soil energy balance model for two extreme conditions with $r_{ss} = 0$ and $r_{ss}\sim\infty$ using meteorological forcing solely, and by approaching the middle point from the two (wet and dry) reference points. Two different methods are then investigated to estimate the pair $(\theta_{1/2} ; \Delta\theta_{1/2}^{-1})$ either from the time series of SEE and $\theta$ observations for a given site, or using the soil texture information for all sites. The first method is based on an algorithm specifically designed to accomodate for strongly nonlinear $\text{SEE}(\theta)$ relationships and potentially large random deviations of observed SEE from the mean observed $\text{SEE}(\theta)$. The second method parameterizes $\theta_{1/2}$ as a multi-linear regression of clay and sand percentages, and sets $\Delta\theta_{1/2}^{-1}$ to a constant mean value for all sites. The new model significantly outperformed the evaporation modules of ISBA (Interaction Sol-Biosph\`{e}re-Atmosph\`{e}re), H-TESSEL (Hydrology-Tiled ECMWF Scheme for Surface Exchange over Land), and CLM (Community Land Model). It has potential for integration in various land-surface schemes, and real calibration capabilities using combined thermal and microwave remote sensing data.