Showing papers by "University of Cambridge published in 1981"

Establishment in culture of pluripotential cells from mouse embryos

Abstract: Pluripotential cells are present in a mouse embryo until at least an early post-implantation stage, as shown by their ability to take part hi the formation of chimaeric animals1 and to form teratocarcinomas2. Until now it has not been possible to establish progressively growing cultures of these cells in vitro, and cell lines have only been obtained after teratocarcinoma formation in vivo. We report here the establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts. These cells are able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo. They have a normal karyotype.

High Energy Astrophysics

Abstract: Part I. Astronomical Background: 1. High energy astrophysics - an introduction 2. The stars and stellar evolution 3. The galaxies 4. Clusters of galaxies Part II. Physical Processes: 5. Ionisation losses 6. Radiation of accelerated charged particles and bremsstrahlung of electrons 7. The dynamics of charged particles in magnetic fields 8. Synchrotron radiation 9. Interactions of high energy photons 10. Nuclear interactions 11. Aspects of plasma physics and magnetohydrodynamics Part III. High Energy Astrophysics in our Galaxy: 12. Interstellar gas and magnetic fields 13. Dead stars 14. Accretion power in astrophysics 15. Cosmic rays 16. The origin of cosmic rays in our galaxy 17. The acceleration of high energy particles Part IV. Extragalactic High Energy Astrophysics: 18. Active galaxies 19. Black holes in the nuclei of galaxies 20. The vicinity of the black hole 21. Extragalactic radio sources 22. Compact extragalactic sources and superluminal motions 23. Cosmological aspects of high energy astrophysics Appendix References Index.

A non-disruptive technique for loading calcium buffers and indicators into cells.

Abstract: Present methods for measuring or buffering intracellular free calcium concentrations are almost entirely limited to robust and well anchored cells which can tolerate insertion of ion-selective microelectrodes or microinjection of calcium indicators or buffers into one cell at a time. A very few types of small cells can be loaded with buffers or indicators during controlled lysis, but such procedures grossly perturb membrane integrity and soluble cytoplasmic constituents. Liposome fusion releases only trace quantities of the trapped solute into the cytoplasm and incorporates foreign lipid into the target cell membranes. I now describe a simple technique which loads Ca2+-selective chelators into the cytoplasm of intact cells in suspension and avoids the disadvantages of previous methods. The chelators are made temporarily membrane permeable by masking their four carboxylates with special esterifying groups which then hydrolyse inside the cells, regenerating and trapping the original chelators. The method is demonstrated on red cells, mast cells and lymphocytes.

Testis weight, body weight and breeding system in primates

Abstract: It has long been known that primate species differ greatly in the weight of their testes relative to body weight1. Recently it has been suggested that among the three species of Pongidae (the great apes), the disparity in testes weights is associated with their different breeding systems2–4. Male gorillas and orangutans copulate infrequently, and when a female comes into oestrus she normally mates with only one male. However, in the chimpanzee, several males mate frequently with the oestrous females, so that each male has to deposit enough sperm to compete with the presence of sperm from other males. For the chimpanzee, therefore, we hypothesize that selection will favour the male that can deposit the largest number of sperm; thus the volume of spermatogenic tissue and hence testis size is far greater in the chimpanzee than in the gorilla or orangutan. If this is correct, it implies that primates in which more than one male mates with each oestrous female should have larger testes relative to their body weight than those with single-male breeding systems. We have tested this prediction across a wide range of primates, and the results support the hypothesis. The relative size of testes may, therefore, provide a valuable clue to the breeding system of a primate species.

Solar Interior Structure and Luminosity Variations

Abstract: The assumptions of standard solar evolution theory are mentioned briefly, and the principle conclusions drawn from them are described. The result is a rationalization of the present luminosity and radius of the Sun. Because there is some uncertainty about the interior composition of the Sun, a range of models is apparently acceptable.

Instrumental responding following reinforcer devaluation.

Abstract: In two experiments, hungry rats were given instrumental lever-press training for an appetitive reinforcer and, in addition, were exposed to another type of food which was not contingent on lever pr...

Stage-specific embryonic antigen involves alpha 1 goes to 3 fucosylated type 2 blood group chains.

Abstract: There is much interest in developmentally regulated molecules which may have function in cell interactions and sorting during embryogenesis and differentiation. Numerous antisera have been raised which detect antigens that are expressed in early embryonic cells and become restricted during differentiation, being expressed in only a minority of adult cells (reviewed in refs 1–3). The precise antigenic determinants recognized by such antisera have not been defined. However, studies using a hybri-doma antibody against mouse spleen cells4 and monoclonal autoantibodies of patients with cold agglutinin disease5 have shown that two defined carbohydrate antigen systems, the Forssman and the li antigens, have stage-specific expression in early mouse embryos. We now describe evidence that the stage-specific embryonic antigen SSEA-1 (ref. 6) involves the carbohydrate sequence Galβ1 → 4GlcNAc ↑1,3 Fucα This determinant is formed by α 1 → 3 fucosylation of blood group I or i antigens which are branched or linear oligosaccharides, respectively7–9, built of Galβ1 →4GlcNAc units and known as type 2 precursor chains10 of the major blood group antigens. Thus, we introduce the concept of simple glycosylation changes as a basis for stage-specific expression of embryonic antigens.

Evidence for the dilution effect in the selfish herd from fish predation on a marine insect

Abstract: It has been proposed that a major factor underlying the evolution of gregarious behaviour is a reduction in the risk of being attacked by predators1–3. One way in which individuals may gain protection from predators by joining a group is through a simple ‘dilution’ effect—for any one predator attack, the larger the group of prey animals, the smaller is the chance that any particular individual will be the victim. We present here field observations of predation on a marine insect, in which it is possible, for the first time, to quantify the magnitude of the dilution effect and distinguish it from other benefits of group living.

Efficiency of human visual signal discrimination.

Abstract: We have measured the overall statistical efficiency of human subjects discriminating the amplitude of visual pattern signals added to noisy backgrounds. By changing the noise amplitude, the amount of intrinsic noise can be estimated and allowed for. For a target containing a few cycles of a spatial sinusoid of about 5 cycles per degree, the overall statistical efficiency is as high as 0.7 +/- 0.07, and after correction for intrinsic noise, efficiency reaches 0.83 +/- 0.15. Such a high figure leaves little room for residual inefficiencies in the neural mechanisms that handle these patterns.

Immunohistochemical mapping of vitamin D-dependent calcium-binding protein in brain

Abstract: The steroid hormone, 1,25-dihydroxycholecalciferol (1,25-(OH)2D3) causes the de novo synthesis of a calcium-binding protein (D-CaBP)1,2 This protein is present in highest concentrations in intestine, kidney and shell gland, across which calcium is transported in relatively large amounts, but it is also found in smaller amounts in several other tissues, including brain3–7 The area of brain with the highest D-CaBP concentration is the cerebellum, where the protein is found only in the Purkinje cells7,8 We have now mapped D-CaBP immuno-histochemically throughout the brain of chicks and rats and present here a list of all positive nuclei Because certain of these neurones also contain 1,25-(OH)2D3 (ref 9), we suggest that they are target cells for this hormone, thus broadening the functional significance of vitamin D to include the brain and implicating vitamin D in a more widespread action than simply a role in the calcium translocation mechanism of epithelial cells

An electrical tuning mechanism in turtle cochlear hair cells

Abstract: 1. Intracellular recordings were made from single cochlear hair cells in the isolated half-head of the turtle. The electrical responses of the cells were recorded under two conditions: (a) when the ear was stimulated with low-intensity tones of different frequencies and (b) when current steps were injected through the intracellular electrode. The aim of the experiments was to evaluate the extent to which the cochlea's frequency selectivity could be accounted for by the electrical properties of the hair cells.2. At low levels of acoustic stimulation, the amplitude of the hair cell's receptor potential was proportional to sound pressure. The linear tuning curve, which is defined as the sensitivity of the cell as a function of frequency when the cell is operating in its linear range, was measured for a number of hair cells with characteristic frequencies from 86 Hz to 425 Hz.3. A rectangular current passed into a hair cell elicited a membrane potential change consisting of a damped oscillation superimposed on a step. Small currents produced symmetrical oscillations at the beginning and end of the pulse. Larger currents increased the initial ringing frequency if depolarizing and decreased it if hyperpolarizing.4. For small currents the frequency of the oscillations and the quality factor (Q) of the electrical resonance derived from the decay of the oscillations were close to the characteristic frequency and Q of the hair-cell linear tuning curve obtained from sound presentations.5. The hair cell's membrane potential change to small-current pulses or low-intensity tone bursts could be largely described by representing the hair cell as a simple electrical resonator consisting of an inductance, resistor and capacitor.6. When step displacements of 29-250 nm were applied to a micropipette, placed just outside a hair cell in the basilar papilla, an initial periodic firing of impulses could be recorded from single fibres in the auditory nerve. Currents of up to 1 nA, injected through the same micropipette, failed to produce any change in the auditory nerve discharge. The experiment demonstrates that current injection does not produce gross movements of the electrode tip.7. The contribution of the electrical resonance to hair-cell tuning was assessed by dividing the linear tuning curve by the cell's impedance as a function of frequency. The procedure assumes that the electrical resonance is independent of other filtering stages, and on this assumption the resonance can account for the tip of the acoustical tuning curve.8. The residual filter produced by the division was broad; it exhibited a high-frequency roll-off with a corner frequency at 500-600 Hz, similar in all cells, and a low-frequency roll-off, with a corner frequency from 30 to 350 Hz which varied from cell to cell but was uncorrelated with the characteristic frequency of the cell.9. The phase of the receptor potential relative to the sound pressure at the tympanum was measured in ten cells. For low intensities the phase characteristic was independent of the sound pressure. At low frequencies the receptor potential led the sound by 270-360 degrees , and in the region of the characteristic frequency there was an abrupt phase lag of 90-180 degrees ; the abruptness of the phase change depended upon the Q of the cell.10. The calculated phase shift of the electrical resonator as a function of frequency was subtracted from the phase characteristic of the receptor potential. The subtraction removed the sharp phase transition around the characteristic frequency, and in this frequency region the residual phase after subtraction was approximately constant at +180 degrees . This is consistent with the idea that the hair cells depolarize in response to displacements of the basilar membrane towards the scala vestibuli. The high-frequency region of the residual phase characteristic was similar in all cells.11. It is concluded that each hair cell contains its own electrical resonance mechanism which accounts for most of the frequency selectivity of the receptor potential. All cells also show evidence of a broad band-pass filter, the high frequency portion of which may be produced by the action of the middle ear.

The fluid dynamics of a basaltic magma chamber replenished by influx of hot, dense ultrabasic magma

Abstract: This paper describes a fluid dynamical investigation of the influx of hot, dense ultrabasic magma into a reservoir containing lighter, fractionated basaltic magma. This situation is compared with that which develops when hot salty water is introduced under cold fresh water. Theoretical and empirical models for salt/water systems are adapted to develop a model for magmatic systems. A feature of the model is that the ultrabasic melt does not immediately mix with the basalt, but spreads out over the floor of the chamber, forming an independent layer. A non-turbulent interface forms between this layer and the overlying magma layer across which heat and mass are transferred by the process of molecular diffusion. Both layers convect vigorously as heat is transferred to the upper layer at a rate which greatly exceeds the heat lost to the surrounding country rock. The convection continues until the two layers have almost the same temperature. The compositions of the layers remain distinct due to the low diffusivity of mass compared to heat. The temperatures of the layers as functions of time and their cooling rate depend on their viscosities, their thermal properties, the density difference between the layers and their thicknesses. For a layer of ultrabasic melt (18% MgO) a few tens of metres thick at the base of a basaltic (10% MgO) magma chamber a few kilometres thick, the temperature of the layers will become nearly identical over a period of between a few months and a few years. During this time the turbulent convective velocities in the ultrabasic layer are far larger than the settling velocity of olivines which crystallise within the layer during cooling. Olivines only settle after the two layers have nearly reached thermal equilibrium. At this stage residual basaltic melt segregates as the olivines sediment in the lower layer. Depending on its density, the released basalt can either mix convectively with the overlying basalt layer, or can continue as a separate layer. The model provides an explanation for large-scale cyclic layering in basic and ultrabasic intrusions. The model also suggests reasons for the restriction of erupted basaltic liquids to compositions with MgO<10% and the formation of some quench textures in layered igneous rocks.

Generalized descent for global optimization

Abstract: This paper introduces a new method for the global unconstrained minimization of a differentiable objective function. The method is based on search trajectories, which are defined by a differential equation and exhibit certain similarities to the trajectories of steepest descent. The trajectories depend explicitly on the value of the objective function and aim at attaining a given target level, while rejecting all larger local minima. Convergence to the gloal minimum can be proven for a certain class of functions and appropriate setting of two parameters.

Parental investment in male and female offspring in polygynous mammals

Abstract: Two evolutionary theories predict how mammalian parents would be expected to allocate the resources at their disposal to their male and female progeny. Where reproductive success varies more widely among males than females and variation in success among adults is influenced by parental investment1, parents would maximize their reproductive success by allocating a greater proportion of their resources to individual sons than to individual daughters2–5. However, because the benefits of producing offspring of one sex are inversely related to the total investment allocated to them, parents should, on average, divide their total investment equally between their male and female progeny, rearing fewer of whichever sex is individually more expensive to produce6–10. Here we examine the extent to which parental investment in red deer (Cervus elaphus) and other polygynous mammals matches these predictions. We conclude that, in several mammals, mothers invest more heavily in individual sons than daughters but that, contrary to prediction, there is no indication that fewer male offspring are reared in these species.

Morphology and Topography of on- and off-Alpha Cells in the Cat Retina

Abstract: Neurofibrillar staining methods were found to stain all alpha cells of the cat retina completely, that is the perikaryon, the axon and the dendritic branches. The dendrites of the alpha cells in vertical sections were found to be unistratified and to occupy two narrow strata in the outer half of the inner plexiform layer. This difference in branching level could also be observed in whole-mount preparations and it has been demonstrated in the preceding paper (Peichl & Wassle 1981) that it corresponds to the physiological on-off dichotomy. Thus the topographical distribution of on- and off-alpha cells could be studied. They are found to occur in about equal numbers. Both on- and off-alpha cell perikarya form a regular lattice and both lattices are superimposed independently. The dendritic branches of neighbouring alpha cells overlap and each retinal point is covered by the dendritic field of at least one on- and one off-alpha cell. The dendritic trees of on-alpha cells seem to have more small branches and are on the average smaller than those of off-alpha cells. The density of alpha cells was found to peak in the central area whence it continuously decreased towards the retinal periphery.