Showing papers in "Biophysical Chemistry in 2008"

TL;DR: Practical formulae and parameters for prediction of UV spectra, hypochromism and peak wavelengths were experimentally determined and the accuracy of predictions made using the nearest-neighbor model and the base composition model was determined and compared.

TL;DR: The results show that the structure of casein micelles is more compact at low pH and looser at high pH, and the casein micelle has the most compact structure at pH 5.5, when it has almost no electrostatic repulsion between casein molecules.

TL;DR: The susceptible hydroxyl group of these compounds are located in the lipid region of the bilayer close to the double bonds of polyunsaturated fatty acids, making these stilbenes particularly suitable for the prevention and control of the lipid peroxidation of the membranes.

TL;DR: The fluorinated nanoparticles with highly negative zeta potential and hydrophobic fluorinated core have the fundamental characteristics to prevent Abeta fibrillogenesis.

TL;DR: The membrane protein bacteriorhodopsin (BR) can be kept soluble in its native state for months in the absence of detergent by amphipol (APol) A8-35, an amphiphilic polymer as discussed by the authors.

TL;DR: Analytical analyses provide strong evidence that the measured calcium traces in hepatocytes are prevalently of stochastic nature, and the biological importance of this finding is discussed in relation to the mechanisms incorporated in mathematical models as well as the role of stoChasticity and determinism at cellular and tissue levels.

TL;DR: The analysis suggests that the adsorbed peptides give rise to considerable local curvature disruptions of the membrane, which is interpreted in terms of a continuum model describing perturbations on the membrane organization.

TL;DR: Free base porphyrin (PPhe), derivatized with aminosulfonyl groups linked to the aromatic amino acid phenylalanine at the meso-positions, was mixed with DMPC vesicles and the resulting interaction was studied by absorption, steady-state and transient state fluorescence, at different pHs.

TL;DR: Each sugar stabilizes the protein in terms of T(m) and DeltaG(D)(o), and this stabilization is under enthalpic control, which suggests that secondary and tertiary structures of protein in their native and denatured states are not perturbed on the addition of sugars.

TL;DR: The results show that CLAs are more susceptible to peroxidation than LA and LAME, and the data indicate thatCLAs would provide protection against free radicals, but LA andLAME cannot.

TL;DR: A new class of surfactant-cobalt(III) complex ions of the type, cis-[Co(X)(2)(C(14)H(29)NH(2))Cl](2+) (trien=triethylenetetramine) were synthesized and characterized by IR, NMR, UV-visible electronic absorption spectra, elemental analysis and metal analysis.

TL;DR: Application of the Fourier transform algorithm to the G CR2 family revealed strongly predicted seven fold periodicity in hydrophobicity, suggesting why GCR2 has been reported to be a GPCR, despite negative indications in most transmembrane prediction algorithms.

TL;DR: Molecular docking of isoxazolCurcumin and diacetylcurcumin with bovine serum albumin indicated that they docked close to Trp 213, which is within the hydrophobic subdomain.

TL;DR: In this article, it was shown that stable alpha or gamma glycine can be transformed into metastable β-gene in a single step using X-ray diffraction techniques.

TL;DR: The systems studied exhibit features of two of the major schools of thought of lung surfactant mechanisms, in that although unsaturated lipids did not appear to prevent the monolayers from achieving high surface pressure, POPG did appear to be selectively squeezed out of the DPPC/POPG monolayer at high lipid densities.

TL;DR: In this paper, the authors investigated the heat-induced aggregation of α-chymotrypsin and found that α-and β-caseins and α-crystalline could inhibit thermal aggregation of CT, and their inhibitory effect was nearly pH-independent.

TL;DR: This study has been able to show for the first time a direct correlation between protein stability and amyloid formation enhancement by salts from the Hofmeister series, where SO(4)(2-) conferred the mostprotein stability and enhancement of amyloids formation.

TL;DR: Results suggest that AuNP were functionalized with TOC through interactions with the N-terminal amine of the phenylalanine, the amide groups and possibly with the indole group of the tryptophan residue.

TL;DR: The results suggest that the efficiency of DNA photocleavage by these porphyrins shows high dependence on the values of K(b), which are greatly influenced by the number of positive charges and steric hindrance.

TL;DR: The self-assembly in films dried from aqueous solutions of a modified amyloid beta peptide fragment is studied and it is concluded that the average width of an AAKLVFF fibril is (63+/-18) nm, indicating that these fibrils comprise beta-sheets with multiple repeats of the unit cell.

TL;DR: An additive scheme for calculating the partial molar volume and adiabatic compressibility of fully extended polypeptide chains as a function of pH and temperature is developed and suggests that neither apocytochrome c nor apomyoglobin are fully unfolded and retain a sizeable core of solvent-inaccessible groups.

TL;DR: The mapping potential of DS after cytoplasmatic and membranal markers for cell-based screening analysis is demonstrated and differences on the matching dielectric properties of cells were obtained.

TL;DR: The geometries, relative energies, gas-phase static and dynamic dipole polarizabilities of the two most stable neutral forms and of the zwitterionic form of the twenty naturally occurring amino acids have been obtained by Density Functional and conventional ab initio Hatree-Fock theories using correlation consistent basis sets.

TL;DR: The interaction between DNA and cationic gemini surfactant trimethylene-1, 3-bis (dodecyldimethylammonium bromide) (12-3-12) has been investigated by the measurements of fluorescence, surface tension, UV spectrum and circular dichroism.

TL;DR: The experimental results show that at low [Por]/[DNA] ratios, the parent porphyrin binds to DNA in an intercalative mode while the hybrid binds in a combined mode of outside binding and partial intercalation, which can explain the different DNA binding behaviors reasonably.

TL;DR: TLL secondary structure analysis by amide I and amide III vibrational bands showed that content of alpha-helixes does not change, while a part of beta-sheet structures transforms to less ordered elements upon incorporation of protein into the Q230 phase of aqueous phytantriol.

TL;DR: The conditional formal potential, E degrees', of Rutin has been studied by cyclic voltammetry using a Rutin film deposited at the multi-wall carbon nanotubes modified glassy carbon electrode (GCE) as the working electrode in different pH phosphate buffered solutions.

TL;DR: A dynamic Monte Carlo simulation is performed to give a molecular insight into the way in which native proteins aggregate in solution and to explore means of suppressing aggregation, using two proteins of different compositions and conformations represented by a two-dimensional (2D) lattice model (HP model).

TL;DR: The influence of cross-linking on the permittivity of collagen is significant over the entire temperature range and is associated with changes in the secondary structure of collagen accompanied by the release of water and with the denaturation of dry collagen, respectively.

TL;DR: Electrochemical parameters of immobilized myoglobin such as formal potential, charge transfer coefficient, and apparent heterogeneous electron transfer rate constant were estimated by cyclic voltammetry and nonlinear regression analysis and biocatalytic activity was exemplified at the prepared electrode for reduction of hydrogen peroxide.