Showing papers in "Experimental Cell Research in 1984"

TL;DR: When compared with the phenotypes of isolated and purified normal hepatocytes, biliary epithelial (ductular) cells and 'oval' cells isolated from livers treated with chemical carcinogens, the phenotypic properties of the liver epithelial cell line in culture most resemble those of the 'oval'.

TL;DR: Bombesin and the C-terminal portion of gastrin-releasing peptide each increase clonal growth rate and colony-forming efficiency of normal human bronchial epithelial cells in the presence or absence of an optimal concentration of epidermal growth factor (EGF).

TL;DR: A pluripotent, karyotypically normal, male culture line ESC-BLC 1 of embryonal stem cells was established from delayed mouse blastocysts of strain 129/ter Sv and determination of alkaline phosphatase yielded a high specific activity.

TL;DR: The emergence of diversified cell subpopulations in malignant tumors insures that some cells will ultimately become highly metastatic, resulting in tumor progression towards characteristics which are the most favorable for survival and growth.

TL;DR: A new approach is presented for the indirect hybridocytochemical localization of specific nucleic acid sequences in microscopic preparations, based on the application of probes modified with N-acetoxy-2-acetylaminofluorene.

TL;DR: The evidence that centrosomes are flexible bodies is considered; they may take on alternative forms and their forms determine the shapes of mitotic poles and other organizers of microtubular structures.

TL;DR: A rabbit antiserum against a purified embryonal carcinoma (EC) cell surface glycoprotein interferes with cell-cell interaction in mouse preimplantation embryos and seems not to be an integral membrane component.

TL;DR: A comparison of protein compositions of mouse late-step spermatids and cauda epididymal sperm has revealed that the relative distribution of the two amino acid sequence variants of mouse protamine differ markedly in sper matids and sperm as discussed by the authors.

TL;DR: The average pH within endocytic compartments was determined during endocytosis of fluorescein conjugates by macrophages and hepatocytes as mentioned in this paper, and the half-time for this process was generally less than 4 min.

TL;DR: The additive nature of the FGF and serum-stimulatory activity suggests that FGF-like molecules are not the active agents in serum and that more than one pathway may be involved in stimulating satellite cell proliferation.

TL;DR: The data indicate that the architecture of two discrete cytoskeletal elements (microtubules and microfilaments) is regulated directly by separate serum growth factors (insulin and PDGF), however the disruption of microfilament architecture by PDGF does not seem necessary for induction of cell growth and the disruptionof microtubule filaments by insulin is not sufficient.

TL;DR: These observations not only suggest a convenient method for fractionating matrix structures from rat liver nuclei into biochemically and morphologically discrete components, but also identify a subset of major non-lamin, non-histone nuclear polypeptides whose intermolecular interactions can be reversibly stabilized apparently by intermolescular disulfide bond formation by NaTT.

TL;DR: It is suggested that the principal role of ascorbic acid in the cell cycle may be related to its action in controlling the synthesis of hydroxyproline-containing proteins, which can be essential requirements for development of G1 and G2.

TL;DR: P pH was derived accurately from spectra using the simple Henderson-Hasslebach equation and macrophages were permitted to pinocytose FITC and TRITC dextran conjugates simultaneously.

TL;DR: En se referrant a divers travaux, il semble que la croissance cellulaire, suivie par la taille de la cellule, la quantite de proteines ou d'ARN ribosomaux, ne determine pas la replication de l'ADN

TL;DR: Evidence is provided that nucleolar phosphoprotein C23 is the major silver staining protein of the nucleolus and that it is directly or indirectly associated with rDNA.

TL;DR: Results show that newly administered [3H]retinol, which is initially located in the hepatocytes, is transferred to the stellate cells and stored there.

TL;DR: Indirect immunofluorescence microscopy with monoclonal antibody against DNA polymerase alpha revealed the intranuclear localization of DNA polymerases alpha in G1, S, and G2 phases of transformed human cells, and dispersed cytoplasmic distribution during mitosis.

TL;DR: This perfusion method is shown to enable Chinese hamster lung fibroblasts (CHLF) to remain in a metabolically active state with high levels of intracellular ATP for many hours.

TL;DR: It is concluded that Kupffer cells in monolayer culture take up liposomes primarily by way of an adsorptive endocytic mechanism.

TL;DR: This chapter discusses the results of the work on exocytosis in Paramecium as well as the implications of these results for exocyTosis in general.

TL;DR: In frozen sections of cornea and skin, plectin was found to be enriched at epithelial basal cell surface membranes, which could serve as a tool in the classification of mechanobullous diseases.

TL;DR: It is concluded that interaction of mammary epithelium with adipocytes results in a marked increase in proliferation of mammaries and that extracellular components may mediate this effect.

TL;DR: It is suggested that microtubule-disrupting drugs induce motility via structural changes in the cytoskeleton which act as signals for the motor apparatus which could be reversed by an extracellular chemotactic gradient.

TL;DR: The data suggest that glycolipids, particularly GM3, at the cell attachment site have different metabolic activity from those of whole cells; the label in glycolIPids goes preferentially into cell attachment sites, and may have some functional role in regulating cell attachment of BHK cells.

TL;DR: Results suggest that during in vitro senescence, the increase in cell size is correlated with increased organization of the cytoskeleton, and the presence of a rigid cytoskeletal structure may contribute in part to the inability of the senescent cell to replicate.

TL;DR: It is suggested that serum deprivation sensitizes the cells to an unidentified serum component which sets the cells back in G1, unless insulin is present to maintain the flow of cells into S.

TL;DR: It is shown that cadmium induced the synthesis of 'heat shock proteins' (hsps), which started after 6 h and was maximal after 24 h of 50-100 microM CdCl2 treatment.

TL;DR: The data from the two species are consistent with the proposal that cultured endothelial cells produce at least two distinct mitogens, one of which is biochemically and immunologically related to PDGF.

TL;DR: These studies demonstrate for the first time that monoclonal antibodies may be used in combination with immunogold electron microscopy to identify the ultrastructural location of nuclear antigens.