Showing papers in "Molecular Brain Research in 1994"

TL;DR: The results confirm and extend previous observations that 5-HT2C receptor mRNA is present in many brain areas in addition to those autoradiographically shown to have the corresponding binding sites and that 4-HT1C receptor subtype is a principal 5- HT receptor in the brain.

TL;DR: The RT-PCR results support the idea that one pathway by which peripheral immune stimuli affect brain functions includes local synthesis of proinflammatory cytokines in certain brain structures.

TL;DR: These mRNA distributions largely agree with previous findings on the alpha 2-adrenoceptor distributions in the rat brain, but suggest that the localization patterns for each receptor subtype are unique.

TL;DR: In situ hybridization studies indicate that the mRNA for Hyp 8-1 is distributed discretely throughout the rat brain, in an overall pattern which is different from that of several other G-protein-coupled seven transmembrane receptors, suggesting that it is likely that the Hyp 7-1 cDNA encodes a novel peptide receptor.

TL;DR: It is demonstrated that the inflammatory cytokine IL-1β is induced in a time-dependent way after brain ischaemia.

TL;DR: Several lines of evidence indicate that astrocytes constitute the major cell type responsive to 1,25-(OH)2 D3 in primary cultures of glial cells, possibly as a consequence of the increase of the VDR gene expression.

TL;DR: Brains from 5 patients with Alzheimer's disease showed a 50%-65% decrease in mRNA levels of the mitochondrial-encoded cytochrome oxidase subunits I and III in the middle temporal association neocortex, but not in the primary motor cortex, as compared to 5 control brains, suggesting that the decrease in COX I andIII subunits mRNA in affected brain regions may contribute to reduced brain oxidative metabolism in AD.

TL;DR: C-Jun showed a prolonged expression in neurons undergoing delayed, but not necrotic, cell death suggesting that they may be involved in the biochemical cascade that causes selective delayed neuronal death.

TL;DR: The findings suggest the possibility that IL-1 produces its multiple effects on the central nervous system through the actions not only on neuronal cells but also on endothelial and epithelial cells.

TL;DR: The present study used to unmodified antisense oligodeoxynucleotides (ODNs) to disrupt the synthesis of NPY in the ARC and to examine the impact of this disturbance on nutrient intake, as well as on circulating levels of insulin and the adrenal steroids, corticosterone and aldosterone.

TL;DR: Differential induction of IEGs by HAL and CLOZ was also observed in the lateral septal nucleus and the islands of Calleja complex of the rat brain, which may be related to the different clinical profiles of the two drugs.

TL;DR: A putative BK channel gene was cloned from a human brain (substantia nigra) cDNA library by hybridization screening and high homology with the mSlo gene suggests that this cDNA is the human homologue (hSlo).

TL;DR: Evidence is provided for induction of GLUT1 and GLUT3 gene expression in malignant glial cells, and the mRNA levels correlate with the biologic aggressiveness of the tumor.

TL;DR: Oligonucleotide probes were synthesized and used in RT-PCR followed by Southern blotting to show that the whole brain expresses transcripts for both the type I and type II IL-1 receptors, and offer further support to the idea that type I IL- 1 receptors are synthesised and expressed by neurons.

TL;DR: The results suggest that three zebrafish otx homeoproteins, alone or in combination, may play roles in very early embryogenesis, gastrulation, and the development and subdivision of the diencephalon and the midbrain.

TL;DR: Double labelling demonstrated that in sDH most of the NDP-reactive neurons show a close spatial relationship to fibers and varicosities immunoreactive for substance P and CGRP, suggesting a functional relationship between expression of immediate-early gene encoded transcription factors and presence of nitric oxide in the rat spinal cord.

TL;DR: The data suggest that an upregulation of ICAM-1 mRNA and protein on brain capillary endothelium may play an important role in leukocyte migration into ischemic brain tissue.

TL;DR: Using histological procedures which allow for spatial resolution, this study demonstrated that the crucial catecholaminergic neuron-specific DNA element(s) resides between -9 kb and -2.4 kb of the 5' flanking region of the rat TH gene; this assertion is substantiated by the high-level of tissue-specific expression of lacZ in catechlaminergic cells.

TL;DR: Comparative analysis of the distributions of these receptors with that of the components of their putative downstream signal transduction mechanisms suggests that mGLUR5 may be the main subtype of mGluR which mediates the excitatory actions of glutamate in CA1 and could contribute to the elevation of calcium levels found inCA1 pyramidal neurons in long term potentiation and in ischemic/hypoxic injury.

TL;DR: The relative lower expression of the DAT gene in VTA neurons suggests a less efficient dopamine reuptake capacity, which may partly account for the relative sparing of the mesolimbic system reported in Parkinson's disease and MPTP-treated animals.

TL;DR: The results suggest that the induction of sgk gene may be associated with a series of axonal regenerations after brain injury, and in addition, the sgK gene may also play important roles in the development of particular groups of neurons in the postnatal brain.

TL;DR: Treatment of diabetic rats with NGF prevented the deficits in the levels of CGRP and gamma-PPT mRNA in the lumbar dorsal root ganglia, and treatment of non-diabetic rats with a similar NGF regime raised the mean peptide levels to a value similar to that seen in treated diabetic rats but the change was not statistically significant.

TL;DR: A wide spread distribution in the expression of the vasopressin V1aR mRNA in the CNS of male and female rats is demonstrated, and new information is provided on the possible pre- and post-synaptic location of these neuropeptide receptors.

TL;DR: Results suggest that GAT1, GAT2 and GAT3 are expressed in different cells and that G AT1 and G AT3 are involved in distinct GABAergic transmission while GAT 2 may be related to non-neuronal function.

TL;DR: Given the postulated involvement of nitric oxide in neurodegeneration, the widely varying levels of expression of NOS within identified central cholinergic neurons may relate to differential vulnerability of this phenotype in disease or aging.

TL;DR: This study identifies a surprisingly large number of cells that express aromatase mRNA in the zebra finch telencephalon, suggesting that this pattern may be a unique feature of all songbirds.

TL;DR: The finding that postnatal handling significantly increased glucocorticoid receptor mRNA in all hippocampal cell fields is consistent with the results of receptor binding studies showing that handling increases hippocampal glucocortex receptor, but not mineralocortioid receptor density.

TL;DR: It is demonstrated that chronic environmental manipulations alter hippocampal GR gene expression in adult rats, and any relationship between NGF, the transcription factors and GR remains to be determined.

TL;DR: Results showed that mRNA for calbindin-D28K, grp78, and hsp72 increased in the hippocampus following seizures and in the trauma model, expanding the repertoire of known changes in mRNA expression following CNS injury.

TL;DR: The identification of cell types expressing limited combinations of α and β subunits should provide a framework for understanding the physiological significance of Na,K-ATPase isoenzyme diversity and may provide useful tools for the analysis of cell lineage in the mammalian central nervous system.