Showing papers in "Planta Medica in 2013"
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TL;DR: The aim of this review is to provide an update of the new data published on shikonin, whose wide spectrum of pharmacological effects as well as pharmacokinetic properties and toxicity make it a highly interesting target molecule.
Abstract: The naphthoquinone shikonin is the main active principle of Zicao, a
traditional Chinese herbal medicine made from the dried root of
Lithospermum erythrorhizon. Studies carried out over the past 30
years have provided a scientific basis for the use of Zicao which has
been long employed in folk medicine to treat a variety of inflammatory and
infectious diseases. In particular, shikonin has been shown to possess many
diverse properties, including antioxidant, anti-inflammatory,
antithrombotic, antimicrobial, and wound healing effects. The fact that
shikonin shows so many beneficial properties has increased the interest in
this molecule dramatically, especially in the past few years. The aim of
this review is to provide an update of the new data published on shikonin,
whose wide spectrum of pharmacological effects as well as pharmacokinetic
properties and toxicity make it a highly interesting target molecule.
202 citations
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TL;DR: St. John's wort (Hypericum perforatum) has been intensively investigated for its antidepressive activity, but dermatological applications also have a long tradition, and pharmacological research supports the use in these fields.
Abstract: St. Johnʼs wort (Hypericum perforatum) has been intensively
investigated for its antidepressive activity, but dermatological
applications also have a long tradition. Topical St. Johnʼs wort
preparations such as oils or tinctures are used for the treatment of minor
wounds and burns, sunburns, abrasions, bruises, contusions, ulcers, myalgia,
and many others. Pharmacological research supports the use in these fields.
Of the constituents, naphthodianthrones (e.g., hypericin) and
phloroglucinols (e.g., hyperforin) have interesting pharmacological
profiles, including antioxidant, anti-inflammatory, anticancer, and
antimicrobial activities. In addition, hyperforin stimulates growth and
differentiation of keratinocytes, and hypericin is a photosensitizer which
can be used for selective treatment of nonmelanoma skin cancer. However,
clinical research in this field is still scarce. Recently, sporadic trials
have been conducted in wound healing, atopic dermatitis, psoriasis, and
herpes simplex infections, partly with purified single constituents and
modern dermatological formulations. St. Johnʼs wort also has a potential for
use in medical skin care. Composition and stability of pharmaceutical
formulations vary greatly depending on origin of the plant material,
production method, lipophilicity of solvents, and storage conditions, and
this must be regarded with respect to practical as well as scientific
purposes.
167 citations
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TL;DR: Berberine may have beneficial effects in the control of blood lipid levels, however, the efficacy of berberine in treating hyperlipidemia should be further evaluated by more randomized controlled trials in a larger population of patients.
Abstract: Clinical trials have reported lipid-lowering effects of berberine intake, but
the findings have been inconsistent. The aim of this meta-analysis was to
assess the safety of berberine and its effects on blood lipid profiles. A
systemic review was designed, undertaken and reported in accordance with the
PRISMA statement. Randomized controlled trials of the effects of berberine
on blood lipids in adults were included. Study population characteristics
and the main results, including changes in the levels of total cholesterol,
triglycerides, low-density and high-density lipoprotein cholesterol, were
extracted. Weighted mean differences were calculated for net changes in
blood lipid concentrations using fixed-effect or random-effects models.
After filtering, eleven randomized controlled trials (including a total of
874 participants) were included in this study. The methodological quality of
these studies was generally low. The final analysis showed that
administration of berberine produced a significant reduction in total
cholesterol (mean difference − 0.61 mmol/L; 95 % confidence interval − 0.83
to − 0.39), triglycerides (mean difference − 0.50 mmol/L; 95 % confidence
interval − 0.69 to − 0.31), and low-density lipoprotein cholesterol (mean
difference − 0.65 mmol/L; 95 % confidence interval − 0.76 to − 0.54) levels,
with a remarkable increase in high-density lipoprotein (mean difference
0.05 mmol/L; 95 % confidence interval 0.02 to 0.09). No serious adverse
effects of berberine have been reported. In conclusion, berberine may have
beneficial effects in the control of blood lipid levels. However, the
efficacy of berberine in treating hyperlipidemia should be further evaluated
by more randomized controlled trials in a larger population of patients.
136 citations
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TL;DR: The current study discloses the chemical nature of different olive materials in a successive and integrated way and reveals new sources of high added value constituents of olives.
Abstract: The aim of the current study was the qualitative exploration and quantitative
monitoring of key olive secondary metabolites in different production steps
(drupes, paste, first and final oil) throughout a virgin olive oil
production line. The Greek variety Koroneiki was selected as one of the most
representative olives, which is rich in biological active compounds. For the
first time, an HPLC-Orbitrap platform was employed for both qualitative and
quantitative purposes. Fifty-two components belonging to phenyl alcohols,
secoiridoids, flavonoids, triterpenes, and lactones were identified based on
HRMS and HRMS/MS data. Nine biologically and chemically significant
metabolites were quantitatively determined throughout the four production
steps. Drupes and paste were found to be rich in several components, which
are not present in the final oil. The current study discloses the chemical
nature of different olive materials in a successive and integrated way and
reveals new sources of high added value constituents of olives.
131 citations
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TL;DR: It has been demonstrated that curcumin has beneficial effects on several ocular diseases, such as chronic anterior uveitis, diabetic retinopathy, glaucoma, age-related macular degeneration, and dry eye syndrome.
Abstract: Curcumin (diferuloylmethane) is the main curcuminoid of the popular Indian
spice turmeric (Curcuma longa). In the last 50 years, in vitro
and in vivo experiments supported the main role of polyphenols and
curcumin for the prevention and treatment of many different inflammatory
diseases and tumors. The anti-inflammatory, antioxidant, and antitumor properties of curcumin are
due to different cellular mechanisms: this compound, in fact, produces
different responses in different cell types. Unfortunately, because of its
low solubility and oral bioavailability, the biomedical potential of
curcumin is not easy to exploit; for this reason more attention has been
given to nanoparticles and liposomes, which are able to improve curcuminʼs
bioavailability. Pharmacologically, curcumin does not show any dose-limiting
toxicity when it is administered at doses of up to 8 g/day for three months.
It has been demonstrated that curcumin has beneficial effects on several
ocular diseases, such as chronic anterior uveitis, diabetic retinopathy,
glaucoma, age-related macular degeneration, and dry eye syndrome. The
purpose of this review is to report what has so far been elucidated about
curcumin properties and its potential use in ophthalmology.
98 citations
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TL;DR: 127 antimycobacterial compounds and their antimyCobacterial activities are reported and it is hoped that some of these compounds may eventually develop into effective new drugs against tuberculosis.
Abstract: Tuberculosis, also called TB, is currently a major health hazard due to
multidrug-resistant forms of bacilli. Global efforts are underway to
eradicate TB using new drugs with new modes of action, higher activity, and
fewer side effects in combination with vaccines. For this reason, unexplored
new sources and previously explored sources were examined and around 353
antimycobacterial compounds (Nat Prod Rep 2007; 24: 278–297) 7 have been previously reported. To develop
drugs from these new sources, additional work is required for preclinical
and clinical results. Since ancient times, different plant part extracts
have been used as traditional medicines against diseases including
tuberculosis. This knowledge may be useful in developing future powerful
drugs. Plant natural products are again becoming important in this regard.
In this review, we report 127 antimycobacterial compounds and their
antimycobacterial activities. Of these, 27 compounds had a minimum
inhibitory concentration of < 10 µg/mL. In some cases, the mechanism of
activity has been determined. We hope that some of these compounds may
eventually develop into effective new drugs against tuberculosis.
80 citations
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TL;DR: New structures, synthesis, and bioactivity of Daphniphyllum alkaloids reported in recent years are presented and several inspired organic syntheses were completed.
Abstract: The unique polycyclic fused ring systems of Daphniphyllum alkaloids, along with their extensive bioactivities, make this family of alkaloids especially attractive targets for total synthesis and biogenetic studies. Successive discoveries of new alkaloids with unprecedented skeletons have made a great contribution to structural diversities of alkaloids elaborated by plants of the genus Daphniphyllum. By the end of 2008, more than 200 alkaloids belonging to 14 different skeletal types have been isolated from different parts of plants of thirteen Daphniphyllum species. These alkaloids show cytotoxic, antioxidant, vasorelaxant, and antiplatelet activating factor effects. The plausible biosynthetic pathways for Daphniphyllum alkaloids have been proposed and biomimetic total syntheses of some alkaloids completed. To provide an update of the previous reviews published in 2009, new structures, synthesis, and bioactivity of Daphniphyllum alkaloids reported in recent years are presented in this article. In the meantime, an additional 54 novel alkaloids have been isolated and identified. Among them, some possess unprecedented frameworks. Several inspired organic syntheses were completed.
80 citations
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TL;DR: Over the four different biflavonoid skeletons tested, amentoflavone and robustaflavone are the most promising ones for antidengue drug development, and Sotetsuflavone is the most active compound of this series and is the strongest inhibitor of the Dengue virus NS5 RNA-dependent RNA polymerase described in the literature.
Abstract: Dengue virus is the worldʼs most prevalent human pathogenic arbovirus. There
is currently no treatment or vaccine, and solutions are urgently needed. We
previously demonstrated that biflavonoids from Dacrydium balansae, an
endemic gymnosperm from New Caledonia, are potent inhibitors of the Dengue
virus NS5 RNA-dependent RNA polymerase. Herein we describe the
structure-activity relationship study of 23 compounds: biflavonoids from
D. balansae (1–4) and from D. araucarioides
(5–10), hexamethyl-amentoflavone (11),
cupressuflavone (12), and apigenin derivatives (13–23).
We conclude that 1) over the four different biflavonoid skeletons tested,
amentoflavone (1) and robustaflavone (5) are the most
promising ones for antidengue drug development, 2) the number and position
of methyl groups on the biflavonoid moiety modulate their inhibition of
Dengue virus NS5 RNA-dependent RNA polymerase, and 3) the degree of
oxygenation of flavonoid monomers influences their antidengue potential.
Sotetsuflavone (8), with an IC50 = 0.16 µM, is the most
active compound of this series and is the strongest inhibitor of the Dengue
virus NS5 RNA-dependent RNA polymerase described in the literature.
76 citations
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TL;DR: Insight is provided into which natural compounds activate the Keap1-nuclear factor erythroid 2-related factor 2 pathway and thus might be useful for detoxifying oxidative/electrophilic stress.
Abstract: Nuclear factor erythroid 2-related factor 2 is a master regulator that
promotes transcription of cytoprotective genes in response to
oxidative/electrophilic stress. A large number of natural dietary compounds
are thought to protect against oxidative stress, and a few have been
reported to induce genes involved in antioxidant defense through activating
nuclear factor erythroid 2-related factor 2. Therefore, a library of 54
natural compounds were collected to determine whether they are nuclear
factor erythroid 2-related factor 2 activators and to compare their efficacy
and potency to activate nuclear factor erythroid 2-related factor 2. The
assay utilized AREc32 cells that contain a luciferase gene under the control
of antioxidant response element promoters. Each natural compound was tested
at 13 concentrations between 0.02 and 30 µM. Known nuclear factor erythroid
2-related factor 2 activators tert -butylhydroquinone and
2-cyano-3,12-dioxooleana-1,9-diene-28-imidazolide were used as positive
controls in parallel with the natural compounds. Among the 54 tested natural
compounds, andrographolide had the highest efficacy, followed by
trans -chalcone, sulforaphane, curcumin, flavone, kahweol, and
carnosol, all of which had better efficacy than
tert -butylhydroquinone. Among the compounds tested,
2-cyano-3,12-dioxooleana-1,9-diene-28-imidazolide was the most potent,
having an EC 50 of 0.41 µM. Seven of the natural compounds, namely
andrographolide, trans -chalcone, sulforaphane, curcumin, flavone,
kahweol, and cafestol had lower EC 50 values than
tert -butylhydroquinone but higher than
2-cyano-3,12-dioxooleana-1,9-diene-28-imidazolide. The present study
provides insights into which natural compounds activate the Keap1-nuclear
factor erythroid 2-related factor 2 pathway and thus might be useful for
detoxifying oxidative/electrophilic stress.
73 citations
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TL;DR: In this review, apart from the traditional concepts followed in phytochemistry for the discovery of novel biologically active compounds, recent applications in the field of extraction, analysis, fractionation, and identification of phytoestrogens will be discussed.
Abstract: Phytoestrogens constitute an attractive research topic due to their
estrogenic profile and their biological involvement in womanʼs health.
Therefore, numerous studies are currently performed in natural products
chemistry area aiming at the discovery of novel phytoestrogens. The main
classes of phytoestrogens are flavonoids (flavonols, flavanones),
isoflavonoids (isoflavones, coumestans), lignans, stilbenoids as well as
miscellaneous chemical groups abundant in several edible and/or medicinal
plants, belonging mostly to the Leguminosae family. As for other bioactives,
the detection of new structures and more potent plant-derived phytoestrogens
typically follows the general approaches currently available in the natural
product discovery process. Plant-based approaches selected from traditional
medicine knowledge and bioguided concepts are routinely employed. However,
these approaches are associated with serious disadvantages such as
time-consuming, repeated, and labor intensive processes as well as lack of
specificity and reproducibility. In recent years, the natural products
chemistry became more technology-driven, and several different strategies
have been developed. Structure-oriented procedures and miniaturized
approaches employing advanced hyphenated analytical platforms have recently
emerged. They facilitate significantly not only the discovery of novel
phytoestrogens but also the dereplication procedure leading to the
anticipation of major drawbacks in natural products discovery. In this
review, apart from the traditional concepts followed in phytochemistry for
the discovery of novel biologically active compounds, recent applications in
the field of extraction, analysis, fractionation, and identification of
phytoestrogens will be discussed. Moreover, specific methodologies combining
identification of actives and biological evaluation in parallel, such as
liquid chromatography-biochemical detection, frontal affinity
chromatography-mass spectrometry and pulsed ultrafiltration-MS will also be
presented. Finally, miniaturized methods (microchip and biosensor) will be
also discussed. With the current review, we attempt to give a wide and holistic overview of
the different approaches which could be employed in the discovery of new
phytoestrogens. On the other hand, we anticipate to attract more scientists
to the area of phytoestrogens and to indicate the need of multidisciplinary
concepts.
70 citations
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TL;DR: It is demonstrated that topical application of chlorogenic acid can accelerate the process of excision wound healing by its ability to increase collagen synthesis through upregulation of key players such as tumor necrosis factor-α and transforming growth factor-β1 in different phases of wound healing as well as by its antioxidant potential.
Abstract: This study was undertaken to evaluate the therapeutic effects of topical chlorogenic acid on excision wounds in Wistar rats. A 1 % (w/w) chlorogenic acid or silver sulfadiazine ointment was applied topically once a day for 15 days on full-thickness excision wounds created on rats. The 1 % (w/w) chlorogenic acid ointment had potent wound healing capacity as evident from the wound contraction on the 15th post-surgery day, which was similar to that produced by 1 % (w/w) silver sulfadiazine ointment. Increased rates of epithelialization were observed in the treated rats. It also improved cellular proliferation, increased tumor necrosis factor-α levels during the inflammatory phase (12 h, 24 h, 48 h, and 72 h post-wounding) of wound healing, upregulated transforming growth factor-β1 and elevated collagen IV synthesis in the chlorogenic acid-treated group. The results also indicated that chlorogenic acid possesses potent antioxidant activity by increasing superoxide dismutase, catalase, and glutathione, and decreasing lipid peroxidation. In conclusion, these results demonstrate that topical application of chlorogenic acid can accelerate the process of excision wound healing by its ability to increase collagen synthesis through upregulation of key players such as tumor necrosis factor-α and transforming growth factor-β1 in different phases of wound healing as well as by its antioxidant potential.
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TL;DR: It is indicated that liposomal encapsulation of crocin could increase its antitumorigenic activity and to obtain an optimal dose for use in humans, the formulation merits further investigation.
Abstract: Crocin is a pharmacologically active component of Crocus sativus. It
is an unusual water-soluble carotenoid responsible for the red color of
saffron. In various studies, the anticancer effect of saffron and its
constituents has been established. Polyethylene glycolated nanoliposomes
with a size range up to 200 nm are suitable for encapsulation of cytotoxic
drugs and can target tumors passively through the enhanced permeation and
retention effect. The aim of this study was to develop a nanoliposomal
formulation containing crocin with a higher therapeutic index for the
treatment of cancer. Four formulations of polyethylene glycolated
nanoliposomes containing 25 mg/ml crocin were prepared with hydrogenated soy
phosphatidylcholine, cholesterol, and methoxy-polyethylene glycol
(MW 2000)-distearoylphosphatidylcholine at different molar ratios by a
solvent evaporation method plus extrusion. Then the liposomes were
characterized for their size, zeta potential, crocin encapsulation, release
properties, and in vitro cytotoxicity against C26 colon carcinoma
cells. Based on in vitro results, the best formulation was selected
for an in vivo study, and its antitumor activity was evaluated in
BALB/c mice bearing C26 colon carcinoma. The IC50 of crocin
itself against C26 colon carcinoma was 0.73 mM. The characterization of the
best formulation was as follow: Z-average size: 127.6 ± 1.5 nm;
polydispersity index: 0.087 ± 0.018; zeta potential: − 21.7 mV ± 6.7;
% encapsulation: 84.62 ± 0.59; % release after 168 hours in RPMI 1640
containing 30 % FBS: 16.26 ± 0.01 %. Liposomal crocin at doses of 50 and
100 mg/kg significantly decreased tumor size and increased survival rate
compared with PBS and crocin in buffer (100 mg/kg) groups. The results of
this study indicated that liposomal encapsulation of crocin could increase
its antitumorigenic activity. Thus, to obtain an optimal dose for use in
humans, the formulation merits further investigation.
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TL;DR: The aims of this review are to examine the various molecular pathways by which the NaK targeting can be more deleterious to biologically aggressive cancer cells than to normal cells.
Abstract: Many cancer patients fail to respond to chemotherapy because of the intrinsic
resistance of their cancer to pro-apoptotic stimuli or the acquisition of
the multidrug resistant phenotype during chronic treatment. Previous data
from our groups and from others point to the sodium/potassium pump (the
Na+/K+-ATPase, i.e., NaK) with its highly specific
ligands (i.e., cardiotonic steroids) as a new target for combating cancers
associated with dismal prognoses, including gliomas, melanomas, non-small
cell lung cancers, renal cell carcinomas, and colon cancers. Cardiotonic
steroid-mediated Na+/K+-ATPase targeting could
circumvent various resistance pathways. The most probable pathways include
the involvement of Na+/K+-ATPase β subunits in
invasion features and Na+/K+-ATPase α subunits
in chemosensitisation by specific cardiotonic steroid-mediated apoptosis and
anoikis-sensitisation; the regulation of the expression of multidrug
resistant-related genes; post-translational regulation, including
glycosylation and ubiquitinylation of multidrug resistant-related proteins;
c-Myc downregulation; hypoxia-inducible factor downregulation; NF-κB
downregulation and deactivation; the inhibition of the glycolytic pathway
with a reduction of intra-cellular ATP levels and an induction of
non-apoptotic cell death. The aims of this review are to examine the various
molecular pathways by which the NaK targeting can be more deleterious to
biologically aggressive cancer cells than to normal cells.
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TL;DR: Understanding the mechanisms of action of these natural remedies used for women's health could lead to more efficacious formulations and to the isolation of active components which have the potential of becoming effective medications in the future.
Abstract: Menopausal women suffer from a variety of symptoms, including hot flashes and night sweats, which can affect quality of life. Although it has been the treatment of choice for relieving these symptoms, hormone therapy has been associated with increased breast cancer risk leading many women to search for natural, efficacious, and safe alternatives such as botanical supplements. Data from clinical trials suggesting that botanicals have efficacy for menopausal symptom relief have been controversial, and several mechanisms of action have been proposed including estrogenic, progestogenic, and serotonergic pathways. Plant extracts with potential estrogenic activities include soy, red clover, kudzu, hops, licorice, rhubarb, yam, and chasteberry. Botanicals with reported progestogenic activities are red clover, hops, yam, and chasteberry. Serotonergic mechanisms have also been proposed since women taking antidepressants often report a reduction in hot flashes and night sweats. Black cohosh, kudzu, kava, licorice, and dong quai all either have reported 5-hydroxytryptamine receptor 7 ligands or inhibit serotonin reuptake, therefore have potential serotonergic activities. Understanding the mechanisms of action of these natural remedies used for women's health could lead to more efficacious formulations and to the isolation of active components which have the potential of becoming effective medications in the future.
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TL;DR: Compared with other agarwoods, "Qi-Nan" was different in containing 2-(2-phenylethyl)chromones with unsubstituted chromone rings and exhibited weak inhibitory activities for acetylcholinesterase inhibitors.
Abstract: Five new 2-(2-phenylethyl)chromone derivatives, qinanones A−E
(1–5), together with eight known 2-(2-phenylethyl)chromone
derivatives (6–13), were isolated from the Et2O
extract of high-quality Chinese agarwood “Qi-Nan” originating from
Aquilaria sinensis. The structures of the new
2-(2-phenylethyl)chromones were elucidated by spectroscopic techniques (UV,
IR, 1D and 2D NMR) and MS analyses. In the bioassay for acetylcholinesterase
inhibitors, compounds 1–6, 10, and 12 exhibited weak
inhibitory activities (inhibition percentage ranged from 10 % to 24 % at the
concentration of 50 µg/mL). Compared with other agarwoods, “Qi-Nan” was
different in containing 2-(2-phenylethyl)chromones with unsubstituted
chromone rings.
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TL;DR: The data provided the reliable evidence that paeniflorin and oxypaeoniflora were able to attenuate advanced glycation end products-induced oxidative damage and inflammation in mesangial cells and might have a beneficial effect in the treatment of diabetic nephropathy.
Abstract: Paeonia suffruticosa, an important traditional herbal medicine, has
been reported to prevent the pathogenesis of diabetic nephropathy through
modulating advanced glycation end products-induced inflammatory and
oxidative stress responses. However, little was known about the protective
effect of the two major compounds in P. suffruticosa, paeoniflorin
and oxypaeoniflora, on advanced glycation end products-induced mesangial
cell damage. In the present study, we investigated the protective activities
of paeoniflorin and oxypaeoniflora on advanced glycation end product-induced
oxidative stress and inflammation in mesangial cells HBZY-1. The
IC50 values of paeoniflorin and oxypaeoniflora for inhibiting
2,2′-azinobis-(3-thylbenzothiazoline-6-sulfonic acid) formation were
4.197 × 10−4 M and 1.002 × 10−4 M, respectively.
The pretreatment with paeoniflorin and oxypaeoniflora
(10−8–10−4 M) significantly increased advanced
glycation end product-induced glutathione peroxidase and catalase
activities. In the coculture system of HBZY-1 and macrophages, paeoniflorin
and oxypaeoniflora could inhibit remarkably the migration of macrophages.
Furthermore, paeniflorin and oxypaeniflora attenuated markedly advanced
glycation end products-induced inflammation cytokines interleukin-6 and
monocyte chemoattractant protein-1 levels in ELISA and western blot analysis
in a dose-dependent manner. Taken together, our data provided the reliable
evidence that paeniflorin and oxypaeniflora were able to attenuate advanced
glycation end products-induced oxidative damage and inflammation in
mesangial cells. Paeniflorin and oxypaeniflora might therefore have a
beneficial effect in the treatment of diabetic nephropathy.
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TL;DR: In a screening of Iranian plants for antiprotozoal activity, an n-hexane extract of the roots of Salvia sahendica potently inhibited the growth of Plasmodium falciparum K1 strain and HPLC-based activity profiling led to the identification of seven known and one new abietane-type diterpenoid.
Abstract: In a screening of Iranian plants for antiprotozoal activity, an n-hexane extract of the roots of Salvia sahendica potently inhibited the growth of Plasmodium falciparum K1 strain. Subsequent HPLC-based activity profiling led to the identification of seven known and one new abietane-type diterpenoid. Structure elucidation was achieved by analysis of spectroscopic data including 1D and 2D NMR. The absolute configuration of sahandol (7) and sahandone (8) were assigned by comparison of experimental ECD spectra with calculated ECD data, using time-dependent density functional theory and methanol as the solvent. In vitro biological activity against P. falciparum and Trypanosoma brucei rhodesiense STIB 900 strain and cytotoxicity in rat myoblast (L6) cells were determined. The IC50 values of the compounds ranged from 0.8 µM to over 8.8 µM against P. falciparum, and from 1.8 µM to over 32.3 µM against T. brucei rhodesiense. The cytotoxic IC50 values ranged from 0.5-15.5 µM. Selectivity indices for P. falciparum were 0.1 to 18.2, and 0.1 to 1.2 for T. brucei rhodesiense.
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TL;DR: Although hop extract and especially 8-PN are promising candidates as a relief for climacteric symptoms, data on the safety and efficacy is still scarce.
Abstract: Hop extract is a long used medicinal product and, regarding hormonal activities, in 1999 a number of prenylflavanones have been identified as its major constituents with 8-prenylnaringenin (8- PN) being the main active estrogenic compound. There have been several in vivo studies performed that demonstrate the potential of hop extract and the single compound 8-PN to alleviate climacteric symptoms like osteoporosis, vasomotoric com- plaints, and sexual motivation. On the other hand, onlya fewclinical studies have been performed so far, and these mainly focused on menopausal dis- comforts, especially hot flushes, yielding rather inconclusive results. Despite preferentially acti- vating estrogen receptor α, 8-PN is only slightly uterotrophic, but it also elucidates estrogenic ef- fects on the mammary gland. In conclusion, although hop extract and especially 8-PN are promising candidates as a relief for climacteric symptoms, data on the safety and efficacy is still scarce. " Humulus lupulus L. l " Cannabaceae l " menopause
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TL;DR: The essential oil from the leaves of X. laevigata is chemically characterized by the presence of γ-muurolene, δ-cadinene, germacrene B, α-copaene, Germacrene D, bicyclogermacrene, and (E)-caryophyllene as major constituents and possesses significant in vitro and in vivo anticancer potential.
Abstract: Xylopia laevigata, popularly known as “meiu” and “pindaiba”, is a medicinal plant used in the folk medicine of the Brazilian Northeast for several purposes. The chemical constituents of the essential oil from leaves of X. laevigata, collected from wild plants growing at three different sites of the remaining Atlantic forest in Sergipe State (Brazilian Northeast), were analyzed by GC/FID and GC/MS. The effect of the essential oil samples was assessed on tumor cells in culture, as well on tumor growth in vivo. All samples of the essential oil were dominated by sesquiterpene constituents. A total of 44 compounds were identified and quantified. Although some small differences were observed in the chemical composition, the presence of γ-muurolene (0.60–17.99 %), δ-cadinene (1.15–13.45 %), germacrene B (3.22–7.31 %), α-copaene (3.33–5.98 %), germacrene D (9.09–60.44 %), bicyclogermacrene (7.00–14.63 %), and (E)-caryophyllene (5.43–7.98 %) were verified as major constituents in all samples of the essential oil. In the in vitro cytotoxic study, the essential oil displayed cytotoxicity to all tumor cell lines tested, with the different samples displaying a similar profile; however, they were not hemolytic or genotoxic. In the in vivo antitumor study, tumor growth inhibition rates were 37.3–42.5 %. The treatment with the essential oil did not significantly affect body weight, macroscopy of the organs, or blood leukocyte counts. In conclusion, the essential oil from the leaves of X. laevigata is chemically characterized by the presence of γ-muurolene, δ-cadinene, germacrene B, α-copaene, germacrene D, bicyclogermacrene, and (E)-caryophyllene as major constituents and possesses significant in vitro and in vivo anticancer potential.
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TL;DR: Two new dihydrothiophene-condensed chromones and a new natural chromone, namely oxalicumones A-C (1-3), respectively, were isolated from a culture broth of a marine-derived fungus, PenicilliumOxalicum, and the structure-biological activity relationship of 1 was discussed.
Abstract: Two new dihydrothiophene-condensed chromones and a new natural chromone, namely oxalicumones A-C (1-3), respectively, were isolated from a culture broth of a marine-derived fungus Penicillium oxalicum SCSGAF 0023, Meripilaceae family. The structures of 1-3 and acetylated derivatives of 1 (4-7) were elucidated on the basis of spectroscopic methods and chemical reactions. The absolute configuration of 1 was established by using the modified Mosher ester method and circular dichroism data of in situ formed [Rh-2(OCOCF3)(4)] and [Mo-2(OAc)(4)] complexes. (R)-MTPA ester of 1 showed cytotoxicity against A375, SW-620, and HeLa carcinoma cell lines with IC50 values of 8.9, 7.8, and 18.4 mu M, respectively. Compound 1 displayed cytotoxicity against A375 and SW-620 cell lines with IC50 values of 11.7 and 22.6 mu M, respectively. The structure-biological activity relationship of 1 is discussed.
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TL;DR: The results, along with the low toxicity of the (+)-limonene epoxide, suggest that this natural compound might be promising for the development of new schistosomicidal agents.
Abstract: Blood fluke of the genus Schistosoma are the etiological agents of
human schistosomiasis, an important neglected tropical disease that afflicts
over 200 million people worldwide. The treatment for this disease relies
heavily on a single drug, praziquantel. Recent reports of praziquantel
resistance raise concerns about future control of the disease and show the
importance of developing new antischistosomal drugs. Currently, natural
products have been a good source for drug development. (+)-Limonene epoxide
is a mixture of cis and trans isomers found in many plants.
Here, we report the in vitro effect of this natural compound on the
survival time of Schistosoma mansoni adult worms. In addition, we
examined alterations on the tegumental surface of adult schistosomes by
means of confocal laser scanning microscopy. The effects of (+)-limonene
epoxide at 25 µg/mL on S. mansoni adult worms were similar to those
of the positive control (praziquantel), with reduction in motility and death
of all worms after 120 h. Confocal laser scanning microscopy revealed that
(+)-limonene epoxide-mediated worm killing was associated with tegumental
destruction. Our results, along with the low toxicity of the (+)-limonene
epoxide, suggest that this natural compound might be promising for the
development of new schistosomicidal agents.
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TL;DR: It is demonstrated that silymarin enhances hepatic glutathione generation by elevating cysteine availability via an increment in Cysteine synthesis and an inhibition of its catabolism to taurine, which may subsequently contribute to the antioxidant defense of liver.
Abstract: It has been known that silymarin exhibits protective activity against
oxidative liver injury induced by various hepatotoxicants, but the
underlying mechanism of its beneficial action remains unclear. We determined
the alterations in sulfur-containing amino acid metabolism induced by
silymarin in association with its effects on the antioxidant capacity of
liver. Male mice were treated with silymarin (100 or 200 mg/kg, p. o.) every
12 h for a total of 3 doses, and sacrificed 6 h after the final dosing. The
hepatic methionine level was increased, but the activity and protein
expression of methionine adenosyltransferase were decreased by silymarin in
a dose-dependent manner. S-Adenosylmethionine or homocysteine
concentration was not changed, whereas the sulfur-containing metabolites
generated from homocysteine in the transsulfuration pathway including
cystathionine, cysteine, and glutathione were increased significantly.
Cystathionine β-synthase was induced, but cysteine dioxygenase was
downregulated, both of which would contribute to the elevation of cysteine
and its product, glutathione, in liver. Oxygen radical scavenging capacity
of liver cytosol against peroxyl radical and peroxynitrite was increased,
and also hepatic lipid peroxidation was diminished in the silymarin-treated
mice. Taken together, the results demonstrate that silymarin enhances
hepatic glutathione generation by elevating cysteine availability via an
increment in cysteine synthesis and an inhibition of its catabolism to
taurine, which may subsequently contribute to the antioxidant defense of
liver.
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TL;DR: Through complete scans, plants metabonomics addresses some of the shortfalls of single analyses and presents a considerable potential to become a sharp tool for traditional Chinese medicine quality assessment.
Abstract: The curative effects of traditional Chinese medicines are principally based
on the synergic effect of their multi-targeting, multi-ingredient
preparations, in contrast to modern pharmacology and drug development that
often focus on a single chemical entity. Therefore, the method employing a
few markers or pharmacologically active constituents to assess the quality
and authenticity of the complex preparations has a number of severe
challenges. Metabonomics can provide an effective platform for complex
sample analysis. It is also reported to be applied to the quality analysis
of the traditional Chinese medicine. Metabonomics enables comprehensive
assessment of complex traditional Chinese medicines or herbal remedies and
sample classification of diverse biological statuses, origins, or qualities
in samples, by means of chemometrics. Identification, processing, and
pharmaceutical preparation are the main procedures in the large-scale
production of Chinese medicinal preparations. Through complete scans, plants
metabonomics addresses some of the shortfalls of single analyses and
presents a considerable potential to become a sharp tool for traditional
Chinese medicine quality assessment.
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TL;DR: Novel findings suggest that geraniin and corilagin from G. thunbergii may be effective therapeutic agents for further drug development in Alzheimer's disease, and were relatively specific and selective inhibitors of β-secretase.
Abstract: Generation of amyloid β peptide through the proteolytic process of amyloid precursor protein by β-secretase and γ-secretase is a main casual factor of Alzheimer's disease, since amyloid β peptide is a major and crucial component of senile plaques in Alzheimer's disease brains In the process of searching for β-secretase inhibitors from natural resources, the EtOAc soluble fraction of Geranium thunbergii exhibited significant β-secretase inhibitory activity Two compounds, geraniin and corilagin, isolated from the most active EtOAc fraction of G thunbergii, exhibited predominant inhibition against β-secretase with IC₅₀ values of 40 × 10⁻⁶ M and 34 × 10⁻⁵ M, respectively Dixon plot of geraniin and corilagin demonstrated that the β-secretase inhibition was noncompetitive with the substrate, thus clearly suggesting that these compounds might bind either to the β-secretase subsites or to another regulatory domain with Ki values of 28 × 10⁻⁶ M and 79 × 10⁻⁵ M, respectively Both compounds exhibited no significant inhibition against α-secretase and other serine proteases including trypsin and chymotrypsin, showing that they were relatively specific and selective inhibitors of β-secretase These novel findings suggest that geraniin and corilagin from G thunbergii may be effective therapeutic agents for further drug development in Alzheimer's disease
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TL;DR: Results in n-STZ diabetic rats loaded with maltose showed that Malmea and Acosmium extracts decreased plasma glucose significantly from 30 min on resembling the effect of acarbose, which contributes to understand the mechanism of action of these plants on glucose metabolism.
Abstract: Type 2 diabetes is an endocrine disease, which accounts for 9% of deaths worldwide. The aim of oral therapy is to reach normoglycemia to prevent later complications. Among glucose-lowering medications, alpha-glucosidase inhibitors delay the absorption of ingested carbohydrates, reducing the postprandial glucose and insulin peaks. In the present study, we tested the butanolic extracts of four Mexican plants with respect to their alpha-glucosidase inhibition activity, without excluding other possible mechanisms of action. The plants Cecropia obtusifolia Bertol., Equisetum myriochaetum Schlecht & Cham, Acosmium panamense (Benth.) Yacolev and Malmea depressa (Baill) R.E. Fries are used in traditional medicine to treat type 2 diabetes. In previous studies, we have demonstrated these plants' hypoglycemic activity and determined the phytochemical composition of their extracts. Our results in n-STZ diabetic rats loaded with maltose showed that Malmea and Acosmium extracts decreased plasma glucose significantly from 30 min on resembling the effect of acarbose. Cecropia extract produced the highest reduction of plasma glucose, and at 90 min, the glucose level was lower than the fasting level, which suggests another mechanism of action. Equisetum did not exert any effect. In vitro assays of alpha-glucosidase activity showed an IC(50) of 14 microg/ml for Cecropia, 21 microg/ml for Malmea, and 109 microg/ml for Acosmium, which were lower than that of acarbose (128 microg/ml). Equisetum did not show any significant effect on this assay, either. These results contribute to understand the mechanism of action of these plants on glucose metabolism.
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TL;DR: A metabolite profiling study was performed in order to obtain a comprehensive picture of the constituents in B. pinnatum leaves and to identify chromatographic markers for quality control and safety assessment of medicinal preparations.
Abstract: Bryophyllum pinnatum is a succulent perennial plant native to Madagascar which is used in anthroposophical medicine to treat psychiatric disorders and as a tocolytic agent to prevent premature labour. We performed a metabolite profiling study in order to obtain a comprehensive picture of the constituents in B. pinnatum leaves and to identify chromatographic markers for quality control and safety assessment of medicinal preparations. Preliminary HPLC-PDA-ESIMS analyses revealed that flavonoid glycosides were the main UV-absorbing constituents in the MeOH extract of B. pinnatum. Two phenolic glucosides, syringic acid β-D-glucopyranosyl ester (1) and 4'-O-β-D-glucopyranosyl-cis-p-coumaric acid (2), as well as nine flavonoids (3-11) including kaempferol, quercetin, myricetin, acacetin, and diosmetin glycosides were unambiguously identified by 1H and 2D NMR analysis after isolation from a MeOH extract. The flavonol glycosides quercetin 3-O-α-L-arabinopyranosyl-(1 → 2)-α-L-rhamnopyranoside 7-O-β-D-glucopyranoside (3) and myricetin 3-O-α-L-arabinopyranosyl-(1 → 2)-α-L-rhamnopyranoside (4) were new natural products. With the aid of HPLC-PDA-APCIMS and authentic references isolated from the related species B. daigremontianum, the presence of four bufadienolides, bersaldegenin-1-acetate (12), bryophyllin A (13), bersaldegenin-3-acetate (14), and bersaldegenin-1,3,5-orthoacetate (15) was detected in B. pinnatum.
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TL;DR: The major ingredient of ginger, [6]-gingerol, could inhibit angiotensin II type 1 receptor activation, which partially clarified the mechanism of ginger regulating blood pressure and strengthening heart in the cardiovascular system.
Abstract: Considering the prevalence of cardiovascular disease in public health and the
limited validated therapeutic options, this study aimed to find novel
compounds targeting the angiotensin II type 1 receptor, accepted as a
therapeutic target in cardiovascular disease. A small library consisting of
89 compounds from 39 Chinese herbs was profiled using a cell-based calcium
mobilization assay which was developed and characterized for high-throughput
screening. [6]-Gingerol derived from Zingiber officinale Roscoe
(ginger) was identified as a novel angiotensin II type 1 receptor
antagonist, with an IC50 value of 8.173 µM. The hit was further
tested by a specificity assay indicating that it had no antagonistic effects
on other evaluated GPCRs, such as endothelin receptors. The major ingredient
of ginger, [6]-gingerol, could inhibit angiotensin II type 1 receptor
activation, which partially clarified the mechanism of ginger regulating
blood pressure and strengthening heart in the cardiovascular system.
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TL;DR: The present review highlights a plethora of studies focusing on the antidiabetic properties of desert and semidesert (steppic) plants, many of them being used for centuries in traditional medicine by Bedouins living in the arid zones of the Middle East and also by ethnic groups in other arid and semiarid parts of the world.
Abstract: The rapidly increasing incidence of diabetes mellitus is becoming a serious threat to
mankindʼs health in all parts of the world. In fact, known cases reflect only part of the
problem, as many diabetics, especially with type 2 diabetes, are unaware of their disease,
which initially shows no definitive symptoms. Despite the great efforts invested in diabetes
research, its prevalence continues to grow, while current medications do not cover all of the
symptoms and complications of the disease. The present review highlights a plethora of studies
focusing on the antidiabetic properties of desert and semidesert (steppic) plants, many of
them being used for centuries in traditional medicine by Bedouins living in the arid zones of
the Middle East and also by ethnic groups in other arid and semiarid parts of the world. The
review concludes in summarizing the work done on the subject and also in pointing to the yet
existing gaps in diabetes research of desert and steppic plants, and suggests directions for
future exploration.
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TL;DR: The extract of the Nigerian lichen Ramalina farinacea showed inhibitory activity against the respiratory syncytial virus in a preliminary assay, and sekikaic acid clearly interferes with viral replication at a viral post-entry step, which is over 1.3-fold more active than the control ribavirin at 4 hours postinfection addition.
Abstract: The extract of the Nigerian lichen Ramalina farinacea showed inhibitory activity against the respiratory syncytial virus in a preliminary assay. A follow-up chemical investigation of this lichen led to the isolation of thirteen phenolic compounds (1-13), including one new hydroquinone depside, designated 5-hydroxysekikaic acid (1), and one new orsellinic acid derivative, 2,3-dihydroxy-4-methoxy-6-pentylbenzoic acid (8). Their structures were unambiguously determined by analysis of 1D and 2D NMR and mass spectroscopic data, as well as by comparison with literature data. Compound 1 was found to partially convert to a 1,4-benzoquinone derivative (1a) during storage. The antiviral activities of the isolated compounds were evaluated against the respiratory syncytial virus. Among them, sekikaic acid (2) showed potent inhibition towards a recombinant strain rg respiratory syncytial virus (IC50 5.69 µg/mL) and respiratory syncytial virus A2 strain (IC50 7.73 µg/mL). The effect of sekikaic acid on the cell viability of HEp2 and Vero cell lines was investigated, and the time of addition assay revealed that sekikaic acid clearly interferes with viral replication at a viral post-entry step, which is over 1.3-fold more active than the control ribavirin at 4 hours postinfection addition. Furthermore, sekikaic acid did not display virucidal activity at concentrations below the TC50, whereas the parental extract did.
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TL;DR: This review discusses successful strategies and potential pitfalls to assembling a natural product-based library suitable for high-throughput screening and the logistics of moving from an assay hit to pure bioactive compound are discussed.
Abstract: This review discusses successful strategies and potential pitfalls to assembling a natural product-based library suitable for high-throughput screening. Specific extraction methods for plants, microorganisms, and marine invertebrates are detailed, along with methods for generating a fractionated sub-library. The best methods to store, maintain and prepare the library for screening are addressed, as well as recommendations on how to develop a robust high-throughput assay. Finally, the logistics of moving from an assay hit to pure bioactive compound are discussed.