Showing papers in "Virology in 1985"

TL;DR: The complete nucleotide sequence of HPV16 DNA cloned from an invasive cervical carcinoma was determined, and an interruption of the reading frame coding for a suspected E1 protein is revealed.

TL;DR: The RNA-dependent DNA polymerase of the AIDS-associated retrovirus (ARV) gives highest activity with the synthetic template, poly(rA)oligo(dT) and prefers Mg2+ over Mn2+ as a divalent cation.

TL;DR: The observations are compatible with the idea that human H3N2 strains might not be able to cross the species barrier to birds directly, and possibly also not the other way around, without prior reassortment in pigs, which seem to have a broader host range concerning the compatibility of the NP gene in reassortants.

TL;DR: It is shown that monocytes harbor the visna genome; and virus gene expression is as constrained in this cell as it is in glial and epithelial cells, in accord with a Trojan Horse mechanism of virus dissemination in an immunologically responsive host.

TL;DR: A rapid purification procedure has been developed which combines the use of an adenovirus-SV40 hybrid virus which overproduces large T antigen, and immunoaffinity chromatography on an anti-large T monoclonal antibody coupled to protein A Sepharose, allowing the isolation of milligram amounts of large T in excellent yield.

TL;DR: A total of eight human epithelial cell lines derived from the carcinoma of the cervix were examined for the presence of human papillomaviruses types 16 and 18 DNA sequences and all the cell lines contain sequences corresponding to early regions E1, E6, and E7.

TL;DR: The results suggest that ARV will be found in other cells of AIDS patients, besides T cells, and that these cells could be the reservoir for continual virus spread in the host.

TL;DR: Findings implicate the activation of c-myc in the pathogenesis of tumors representing two distinct stages of B-cell differentiation within a single animal species.

TL;DR: The results strongly suggest that maturation of MuLV from "immature" to "mature" form of virus particles is indispensable to virus infectivity.

TL;DR: Antisera prepared against synthetic peptides corresponding to the amino and carboxy termini of human adenovirus type 5 (Ad5) early region 1A (E1A) proteins indicated that Ad5 E1A proteins may function in a complex with cellular polypeptides which includes species of 105K, 68K, 65K, and possibly a large protein of greater than 250K

TL;DR: Characteristic DNA endonuclease digest fragment electropherograms and restriction site maps permitted differentiation and genome structure analysis of 38 orthopoxviruses that included isolates of monkeypox virus from humans and animals, monkeypox white variants, variola, vaccinia, ectromelia, Tatera (gerbil) and raccoon poxviruses, and cowpox and camelpox viruses.

TL;DR: The genome of infectious bursal disease virus (IBDV) strain 002-73 was found to consist of two segments of double-stranded RNA which could encode proteins of MW 125K while the MW of the translated products was 169K suggesting that a precursor-product relationship exists between some of the translation products.

TL;DR: The molecular changes in the hemagglutinin (HA) of influenza B virus associated with adaptation to growth in eggs have now been identified and it is concluded that removal or modification of an oligosaccharide structure at this position is required to attach to and infect the allantois cells of the egg and that this has important implications for the antigenic configuration of the molecule.

TL;DR: The virion proteins of two strains of Ebola virus were compared by SDS-polyacrylamide gel electrophoresis (PAGE) and radioimmunoprecipitation (RIP).

TL;DR: The difference in infectivity between the two phenotypes of AcNPV can be attributed to mechanisms of functional entry, as only BV can enter by the more efficient adsorptive endocytosis.

TL;DR: A model in which flavivirus RF functions as a recycling template for semiconservative and asymmetric replication, on which only one nascent strand is synthesized per cycle is formed.

TL;DR: Heteroduplex formation under nonstringent conditions showed that the substituted sequences are partially homologous to each other, with the regions of nonhomology confined to three distinct areas of 100 to 200 nucleotides.

TL;DR: Immune complex kinase assays showed that in vitro phosphorylation of P190 and P240 occurs primarily at tyrosine residues, a rare observation which may be in part attributed to the c-abl gene translocation from chromosomes 9 to 22 occurring in the vast majority of chronic myelogenous leukemia patients.

TL;DR: The major envelope glycoprotein of the causative agent of Acquired Immune Deficiency Syndrome (AIDS) lymphadenopathy-associated virus (LAV) has been identified and characterized.

TL;DR: The data strongly support the hypothesis that V1 is derived from NV2 by proteolytic cleavage and furthermore indicate that V 1 represents the nonglycosylated carboxy-terminal part of NV2 which contains those sequences which anchor NV2 in the viral membrane.

TL;DR: The molecular weight of undissociated, biologically active TVMV or PVY HC, as determined by high-pressure liquid chromatography (HPLC)-gel permeation chromatography was found to be between 100K and 150K, suggesting that the active molecule is a dimer.

TL;DR: The nucleotide sequences coding for all WN virus structural proteins identified so far are described and a hypothesis concerning the processes involved in the synthesis of all viral structural proteins and the probable orientation of these proteins relative to the endoplasmatic reticulum membrane is discussed.

TL;DR: Measles MoAbs which neutralize canine distemper virus in vitro failed to passively protect CDV-infected mice against disease, suggesting an immune mechanism for in vivo protection different from that implicated in in vitro neutralization.

TL;DR: Analysis of the env-pX-LTR region revealed the 90% homology of the nucleotide sequence with that of HTLV-I in each region, which suggests that the possibility of recent interspecies transmission from monkeys to humans in the endemic area is very small.

TL;DR: The results suggest that recognition of the various sialic acids is an important factor in analysis of the receptor specificity of influenza virus hemagglutinins.

TL;DR: It is suggested that template deficiency is a factor contributing to the attenuated phenotype of poliovirus strains studied and a possible involvement of nucleotide sequences located far upstream from the initiator codon in the control of translation of Poliovirus genome is briefly discussed.

TL;DR: The sequence of the 1491 nucleotides found at the 3' end of the genome of the highly aphid-transmissible (HAT) isolate of tobacco etch virus (TEV) has been determined and the deduced amino acid sequences of the two TEV capsid proteins displayed 98% homology and a 66% Homology with PeMV capside protein.

TL;DR: In this paper, the E1B 21-kDa protein is localized in perinuclear region and the cellular p53 antigen is associated with the E 1B 55-kda protein, but not, or only in small amounts at the cell-cell contacts.

TL;DR: A rapid and sensitive focal immunofluorescence assay (FIA) using monoclonal antibodies or heterologous antisera was employed for detection and biological cloning of viruses capable of inducing viral antigens on cell surfaces and was used successfully with C-type murine leukemia viruses.

TL;DR: The nucleotide sequence of a genomic region of herpes simplex virus type 1 (KOS strain) associated with virus-induced cell fusion has been determined and two syncytia-inducing mutations, syn20 and MP, have been previously mapped to a 504-base pair PstI fragment within these genomic coordinates.