Journal ArticleDOI
4thiouridine-specific spin-labeling of E.coli transfer RNA
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This article is published in Biochemical and Biophysical Research Communications.The article was published on 1970-01-23. It has received 64 citations till now. The article focuses on the topics: RNA editing & RNA-dependent RNA polymerase.read more
Citations
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Distance measurements on spin-labelled biomacromolecules by pulsed electron paramagnetic resonance
Gunnar Jeschke,Yevhen Polyhach +1 more
TL;DR: This work has shown that distance measurements between spin labels if pulsed electron paramagnetic resonance techniques such as electron-electron double resonance (ELDOR) and double-quantum EPR are used are well suited to biomacromolecules with an intrinsic flexibility as distributions of distances can be measured.
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Long-range distance determinations in biomacromolecules by EPR spectroscopy.
Olav Schiemann,Thomas F. Prisner +1 more
TL;DR: P pulsed EPR methods like pulsed electron–electron double resonance (PELDOR) and their applications to spin-labeled biosystems are discussed and their advantages and limitations are discussed.
Journal ArticleDOI
Spin labeling of oligonucleotides with the nitroxide TPA and use of PELDOR, a pulse EPR method, to measure intramolecular distances.
TL;DR: This protocol describes the facile synthesis of the nitroxide spin-label 2,2,5,5-tetramethyl-pyrrolin-1-oxyl-3-acetylene and then its coupling to DNA/RNA through Sonogashira cross-coupling during automated solid-phase synthesis and explains how to perform distance measurements between two such spin-labels on RNA/DNA using the pulsed electron paramagnetic resonance method PELDOR.
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Enzymatic Modification of Transfer RNA
TL;DR: There is still no knowledge about the sequence of modification or trimming events leading to the synthesis of active tRNA, and the role of the modified nucleosides in the biological functions of tRNA is still unknown.
References
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Journal ArticleDOI
Amber suppression: a nucleotide change in the anticodon of a tyrosine transfer RNA.
TL;DR: In certain mutants a single base change alters the meaning of a messenger codon in such a way that, instead of spelling out an amino-acid, it spells out chain termination.
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Nucleotide Sequence of N-Formyl-methionyl-transfer RNA
TL;DR: The complete nucleotide sequence of this tRNA from Escherichia coli has been worked out and it is thought to be the chain-initiating tRNA of protein synthesis in bacterial systems.
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Purification of methionine-, valine-, phenylalanine- and tyrosine-specific tRNA from Escherichia coli.
TL;DR: It has been concluded that these amino acid-specific tRNA's are pure enough to use for structural study, suggesting that methionine-tRNA formed a more rigid secondary structure than other t RNA's.
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The isolation of 4-thiouridylic acid from the soluble ribonucleic acid of Escherichia coli.
TL;DR: E. coli S-RNA prepared from Salmonella typhimurium and Rhodiumalumina catalyst was obtained and infrared spectra were run on a Perkin-Elmer model 21 spectrometer.
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The sequence of phenylalanine tRNA from E. coli
B.G. Barrell,Frederick Sanger +1 more
TL;DR: The sequence of this tRNA is studied but certain results are in conflict with the above sequence but the sequences of the nucleotides are tentatively identified as 2-thiomethyl&isopentenyl adenylic acid.