Open Access
5 Microbial Exopolysaccharides
Avinash Mishra,Bhavanath Jha +1 more
TLDR
EPSs produced by bacteria have great potential, and physicochemical characteristics of EPS decide its possible commercial application ranging from pharmaceutical to foodprocessing, extended to detoxification, bioremediation, paints, biotechnology, and petrochemicals.Abstract:
Microbial polysaccharides are produced in two forms, capsular polysaccharide (CPS) and exopolysaccharide (EPS). EPSs of microbial origin are ubiquitous in nature, have unique properties, and can be isolated from the bacteria in fresh water, marine environment, extreme conditions, and soil ecosystem. Exopolysaccharides are comprised of repeated units of sugar moieties, attached to a carrier lipid, and can be associated with proteins, lipids, organic and inorganic compounds, metal ions, and DNA. Specific functions and precise role of EPSs depend on structural units and ecological niches of the host microorganisms. EPSs produced by bacteria have great potential, and physicochemical characteristics of EPS decide its possible commercial application ranging from pharmaceutical to foodprocessing, extended to detoxification, bioremediation, paints, biotechnology, and petrochemicals. Exploitation of microbial exopolysaccharides is relatively unexplored and research interest is constantly increasing toward isolation, characterization, and applications of novel exopolysaccharides as renewable resources. Downstream processing and genetic engineering for enhanced biosynthesis of EPS require further emphasis.read more
Citations
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Journal ArticleDOI
Biosynthesis of dextran by Weissella confusa and its In vitro functional characteristics.
Irina Rosca,Anca Roxana Petrovici,Dragos Peptanariu,Alina Nicolescu,Gianina Dodi,Mihaela Avadanei,Iuliu Ivanov,Andra-Cristina Bostanaru,Mihai Mares,Diana Elena Ciolacu +9 more
TL;DR: It was demonstrated that the obtained EPS is a dextran, which is suitable as hydrophilic matrix for controlled release of drugs in pharmaceutical industry and has no antimicrobial activity against standard bacterial strains.
Book ChapterDOI
Pectic Oligosaccharides and Other Emerging Prebiotics
TL;DR: This chapter summarizes the scientific literature regarding the manufacture and the evaluation of the properties of this group “emerging prebiotics” and some of them are already commercialized but others are still in the early stages of development.
Journal ArticleDOI
Optimization of extracellular polysaccharide production in Halobacillus trueperi AJSK using response surface methodology
TL;DR: Results of the present study proved that statistical media composition analysis with RSM enhanced the EPS production in Halobacillus trueperi.
Journal ArticleDOI
Two UDP-glucuronic acid decarboxylases involved in the biosynthesis of a bacterial exopolysaccharide in Paenibacillus elgii
TL;DR: Results show that P. elgii can be efficient in forming UDP-xylose, which is then used for the synthesis of xylose-containing exopolysaccharide, a natural multi-antibiotic resistant strain.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Colorimetric Method for Determination of Sugars and Related Substances
TL;DR: In this article, a method was developed to determine submicro amounts of sugars and related substances using a phenol-sulfuric acid reaction, which is useful for the determination of the composition of polysaccharides and their methyl derivatives.
Book
Scanning Electron Microscopy and X-Ray Microanalysis
Joseph I. Goldstein,Dale E. Newbury,J. R. Michael,Nicholas W. M. Ritchie,John Henry J. Scott,David C. Joy +5 more
Journal ArticleDOI
A specific color reaction of methylpentoses and a spectrophotometric micromethod for their determination.
Journal ArticleDOI
Bacterial extracellular polysaccharides involved in biofilm formation.
TL;DR: A summary of the current status of the research into the role of EPS in bacterial attachment followed by biofilm formation and a range of novel techniques that can be used in studies involving biofilm-specific polysaccharides is discussed.