Journal ArticleDOI
A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes
TLDR
Lambda derivatives are described that can be used for cloning DNA fragments of about 20 kilobase pairs generated by restriction enzymes EcoRi, HindIII, BamHI, MboI and BglII.About:
This article is published in Gene.The article was published on 1980-08-01. It has received 320 citations till now. The article focuses on the topics: Restriction fragment & Restriction enzyme.read more
Citations
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Hypervariable 'minisatellite' regions in human DNA.
TL;DR: A probe based on a tandem-repeat of the core sequence can detect many highly variable loci simultaneously and can provide an individual-specific DNA ‘fingerprint’ of general use in human genetic analysis.
Journal ArticleDOI
Lambda replacement vectors carrying polylinker sequences
TL;DR: A new family of lambda replacement vectors and derivatives containing amber mutations and derivatives of the EMBL3 vector allow the application of genetic screening procedures based on selection for the products of homologous recombination events, and for the selective cloning of DNA sequences linked to supF genes.
Journal ArticleDOI
Fused transcript of abl and bcr genes in chronic myelogenous leukaemia
TL;DR: Characterization of an 8-kilobase RNA specific to chronic myelogenous leukaemia shows it to be a fused transcript of the two genes, which is probably involved in the malignant process.
Journal ArticleDOI
λ ZAP: a bacteriophage λ expression vector with in vivo excision properties
TL;DR: A lambda insertion type cDNA cloning vector, Lambda ZAP, has been constructed in this article, which eliminates the need to subclone DNA inserts from the lambda phage into a plasmid by restriction digestion and ligation.
Journal ArticleDOI
A phylogenetic analysis of the mycoplasmas: basis for their classification.
William G. Weisburg,J G Tully,D L Rose,J P Petzel,H Oyaizu,D Yang,Linda Mandelco,J E Sechrest,T G Lawrence,J Van Etten +9 more
TL;DR: Small-subunit rRNA sequences were determined for almost 50 species of mycoplasmas and their walled relatives, providing the basis for a phylogenetic systematic analysis of these organisms.
References
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Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
Journal ArticleDOI
The isolation of structural genes from libraries of eucaryotic DNA
Tom Maniatis,Ross C. Hardison,Elizabeth Lacy,Joyce Lauer,Catherine O'Connell,Diana Quon,Gek Kee Sim,Argiris Efstratiadis +7 more
TL;DR: A procedure for eucaryotic structural gene isolation which involves the construction and screening of cloned libraries of genomic DNA is presented and Restriction mapping and hybridization studies reveal the presence of closely linked beta-globin genes.
Journal ArticleDOI
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.
TL;DR: Extension of this method to concentration and purification of other viruses and nucleic acids, as well as some preliminary mechanistic studies, are discussed.
Journal ArticleDOI
Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12.
Journal ArticleDOI
Charon phages: safer derivatives of bacteriophage lambda for DNA cloning
Frederick R. Blattner,Bill G. Williams,Ann E. Blechl,Katherine Denniston-Thompson,Harvey E. Faber,Lesley Anne Furlong,David Grunwald,Delight O. Kiefer,David D. Moore,James W. Schumm,Edward L. Sheldon,Oliver Smithies +11 more
TL;DR: Three of the Charon vector phages, 3A, 4A, and 16A, have been certified for use as EK2 vector-host systems, when propagated in bulk in a special bacterial host, DP50SupF.