A CBF5 mutation that disrupts nucleolar localization of early tRNA biosynthesis in yeast also suppresses tRNA gene-mediated transcriptional silencing
Ann Kendall,Melissa W. Hull,Melissa W. Hull,Edouard Bertrand,Paul D. Good,Robert H. Singer,David R. Engelke +6 more
TLDR
A possible mechanism for tRNA gene-mediated silencing is suggested in which subnuclear localization of tRNA genes antagonizes transcription of nearby genes by pol II.Abstract:
In the budding yeast, Saccharomyces cerevisiae, actively transcribed tRNA genes can negatively regulate adjacent RNA polymerase II (pol II)-transcribed promoters. This tRNA gene-mediated silencing is independent of the orientation of the tRNA gene and does not require direct, steric interference with the binding of either upstream pol II factors or the pol II holoenzyme. A mutant was isolated in which this form of silencing is suppressed. The responsible point mutation affects expression of the Cbf5 protein, a small nucleolar ribonucleoprotein protein required for correct processing of rRNA. Because some early steps in the S. cerevisiae pre-tRNA biosynthetic pathway are nucleolar, we examined whether the CBF5 mutation might affect this localization. Nucleoli were slightly fragmented, and the pre-tRNAs went from their normal, mostly nucleolar location to being dispersed in the nucleoplasm. A possible mechanism for tRNA gene-mediated silencing is suggested in which subnuclear localization of tRNA genes antagonizes transcription of nearby genes by pol II.read more
Citations
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tRNA biology charges to the front
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TL;DR: This review highlights new findings on the diverse pathways of tRNA maturation, and on the formation and function of a number of modifications, on the regulation of t RNA biosynthesis, quality control tRNA turnover mechanisms, widespread tRNA cleavage pathways activated in response to stress and other growth conditions.
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TL;DR: The experimental and theoretical data on this hierarchy of structures and a key role for the recently discovered topologically associating domains are reviewed and proposed.
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The RNA polymerase III transcription apparatus.
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Four histone variants mark the boundaries of polycistronic transcription units in Trypanosoma brucei
T. Nicolai Siegel,Doeke R. Hekstra,Louise E. Kemp,Luisa M. Figueiredo,Joanna E. Lowell,David Fenyö,Xuning Wang,Scott Dewell,George A. M. Cross +8 more
TL;DR: It is suggested that histone modifications and histone variants play crucial roles in transcription initiation and termination in trypanosomes and that destabilization of nucleosomes by hist one variants is an evolutionarily ancient and general mechanism of transcription initiation, demonstrated in an organism in which general pol II transcription factors have been elusive.
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The many facets of H/ACA ribonucleoproteins.
TL;DR: The multiple functions of H/ACA RNPs appear to be reflected in the complex phenotype of dyskeratosis congenita, with an emphasis on the role of the RNP proteins.
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