Open AccessJournal Article
A deletion map of the human Y chromosome based on DNA hybridization.
Gilles Vergnaud,David C. Page,Marie-Christine Simmler,L.G. Brown,François Rouyer,Bernard Noel,David Botstein,de la Chapelle A,Jean Weissenbach +8 more
TLDR
The results suggest a deletion map of the Y chromosome, in which each of the 23 Y-specific restriction fragments tested can be assigned to one of seven intervals, and the polarity of this map with respect to the long and short arms of theY chromosome is established.Abstract:
The genomes of 27 individuals (19 XX males, two XX hermaphrodites, and six persons with microscopically detectable anomalies of the Y chromosome) were analyzed by hybridization for the presence or absence of 23 Y-specific DNA restriction fragments. Y-specific DNA was detected in 12 of the XX males and in all six individuals with microscopic anomalies. The results are consistent with each of these individuals carrying a single contiguous portion of the Y chromosome; that is, the results suggest a deletion map of the Y chromosome, in which each of the 23 Y-specific restriction fragments tested can be assigned to one of seven intervals. We have established the polarity of this map with respect to the long and short arms of the Y chromosome. On the short arm, there is a large cluster of sequences homologous to the X chromosome. The testis determinant(s) map to one of the intervals on the short arm.read more
Citations
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A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif
Andrew H. Sinclair,Berta P,Berta P,M. S. Palmer,Hawkins,B. Griffiths,Smith Mj,Foster Jw,Foster Jw,A.-M. Frischauf,Robin Lovell-Badge,Peter N. Goodfellow +11 more
TL;DR: A search of a 35-kilobase region of the human Y chromosome necessary for male sex determination has resulted in the identification of a new gene, termed SRY (for sex-determining region Y) and proposed to be a candidate for the elusive testis-d determining gene, TDF.
Journal ArticleDOI
The sex-determining region of the human Y chromosome encodes a finger protein
David C. Page,Rebecca Mosher,Elizabeth M. Simpson,Elizabeth M. C. Fisher,Graeme Mardon,Jonathan R. Pollack,Barbara McGillivray,Albert de la Chapelle,Laura G. Brown +8 more
TL;DR: The possibility that the Y-encoded finger protein is the testis-determining factor is discussed, and models of sex determination accommodating the finding of a related locus on the X chromosome are proposed.
Journal ArticleDOI
A Y chromosome gene family with RNA-binding protein homology: Candidates for the azoospermia factor AZF controlling human spermatogenesis
Kun Ma,John D. Inglis,Andrew M. Sharkey,Wendy A. Bickmore,Robert E. Hill,E.Jane Prosser,R. M. Speed,Eric J. Thomson,Mark A. Jobling,K Taylor,Jonathan Wolfe,Jonathan Wolfe,Howard J. Cooke,Timothy B. Hargreave,Ann C. Chandley +14 more
TL;DR: The isolation and characterization of a gene family located within a deletion in Y chromosome interval 6 is reported, suggesting a possible role in RNA processing or translational control during early spermatogenesis.
Journal ArticleDOI
The human Y chromosome: a 43-interval map based on naturally occurring deletions.
Douglas Vollrath,Simon Foote,Adrienne Hilton,Laura G. Brown,Peggy Beer-Romero,Jonathan S. Bogan,David C. Page +6 more
TL;DR: A deletion map of the human Y chromosome was constructed by testing 96 individuals with partial Y chromosomes for the presence or absence of many DNA loci, and should be useful in identifying Y chromosomal genes, in exploring the origin of chromosomal disorders, and in tracing the evolution of the Y chromosome.
References
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Journal ArticleDOI
Occurrence of a transposition from the X-chromosome long arm to the Y-chromosome short arm during human evolution
TL;DR: DXYS1, a site showing greater than 99% DNA sequence homology between the human X and Y chromosomes, maps to the X long arm and to the Y short arm, suggesting an X–Y transposition during human evolution.
Journal ArticleDOI
Repeated sequence specific to human males
TL;DR: Restriction enzyme analysis and nucleic acid hybridisation show that there is a repeated sequence in human male DNA which is not present in human female DNA.
Journal ArticleDOI
The etiology of maleness in XX men
TL;DR: Many of the data are consistent with the hypothesis that XX males arise through interchange of the testic-determining gene on the Y chromosome and a portion of the X chromosome containing the Xg gene, but actual evidence in favor of this hypothesis is still lacking, and the H-Y antigen data are not easy to explain.
Journal ArticleDOI
Single-copy sequence hybridizes to polymorphic and homologous loci on human X and Y chromosomes.
David C. Page,B de Martinville,D Barker,Arlene R. Wyman,Robert E. White,Uta Francke,David Botstein +6 more
TL;DR: Use of a 4.5-kilobase-pair segment of single-copy DNA from a human genomic library as a hybridization probe of genomic human DNAs revealed allelic Taq I restriction fragments 10.6, 11.8, and 14.6 kb long, which demonstrate homology between single- copy sequences on the human X and Y chromosomes.