Journal ArticleDOI
A filter immuno-plaque assay for the detection of antibody-secreting cells in vitro.
TLDR
A plaque assay has been developed that is based on enzyme immunoassay principles and capable of screening several hundred samples in one day, and may be used to evaluate the optimal conditions required for in vitro immunizations.About:
This article is published in Journal of Immunological Methods.The article was published on 1985-05-23. It has received 93 citations till now. The article focuses on the topics: Immunoassay & Low protein.read more
Citations
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Journal ArticleDOI
Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells.
Cecil Czerkinsky,Gudrun Andersson,Hans-Peter Ekre,Lars-Åke Nilsson,Lars Klareskog,Örjan Ouchterlony +5 more
TL;DR: A reverse modification of ELISPOT assay using nitrocellulose membranes and epitope-specific monoclonal antibodies is described for the detection of single lymphokine-secreting cells.
Journal ArticleDOI
Enzyme-Linked Immunosorbent Assay
TL;DR: Solid-phase immunoassays and ELISA have technically simplified antigen quantitation and antibody detection and are the basis for most modern immunodiagnostic tests for acquired immunodeficiency syndrome (AIDS), hepatitis, allergies, and many other human and animal diseases.
Journal ArticleDOI
Bone marrow is a major site of long-term antibody production after acute viral infection
TL;DR: Using the lymphocytic choriomeningitis virus (LCMV) model, it is found that after resolution of the acute infection, a population of virus-specific plasma cells appears in the bone marrow and constitutes the major source of long-term antibody production.
Journal ArticleDOI
Role of appendix in the development of inflammatory bowel disease in TCR-alpha mutant mice.
TL;DR: It is suggested that ALF, rather than PP, is the priming site of cells involved in the disease process and plays an important role in the development of IBD in TCR-alpha-/- mice.
Journal ArticleDOI
A novel two colour ELISPOT assay. I. Simultaneous detection of distinct types of antibody-secreting cells.
Cecil Czerkinsky,Cecil Czerkinsky,Zina Moldoveanu,Jiri Mestecky,Lars-Åke Nilsson,Örjan Ouchterlony +5 more
TL;DR: A novel assay system has been developed which is based on the ELISPOT methodology and employs a combination of two immunoenzyme visualization systems yielding distinct colour products that permits the simultaneous enumeration of two different types of cell secreting antigenically distinct products.
References
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Journal ArticleDOI
Plaque Formation in Agar by Single Antibody-Producing Cells
Niels K. Jerne,Albert A. Nordin +1 more
TL;DR: Distinct plaques, each of which is due to the release of hemolysin by a single antibody-forming cell, are revealed by complement after incubation, in an agar layer, of a mixture of Sheep red cells and lymphoid cells from a rabbit immunized with sheep red cells.
Journal ArticleDOI
A dot-immunobinding assay for monoclonal and other antibodies
TL;DR: A procedure for the assay of antibodies in sera based on the application of the antigen as a spot to nitrocellulose filters is described, which has the merit of being simpler in operation and more sensitive than comparable existing procedures.
Journal Article
Further improvements in the plaque technique for detecting single antibody-forming cells.
A J Cunningham,A Szenberg +1 more
TL;DR: A simple technique for detecting plaque-forming cells that combines the sensitivity and improved optical conditions of the previously reported monolayer technique with the screening power and ease of quantification of the original agar-plate method is described.
Journal ArticleDOI
A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells
TL;DR: A solid-phase enzyme-linked immunosorbent assay (ELISPOT) is described, which provides a useful alternative to conventional plaque-forming cell assays.
Journal ArticleDOI
A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells
J.D. Sedgwick,Patrick G. Holt +1 more
TL;DR: A sensitive immunoassay is described for the detection of idiotype- and isotype-specific antibody-secreting cells (ASC), based upon the well established principles of ELISA, which has sensitivity and specificity at least equivalent to haemolytic plaque assays.
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A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells
J.D. Sedgwick,Patrick G. Holt +1 more