Journal ArticleDOI
A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells
TLDR
A solid-phase enzyme-linked immunosorbent assay (ELISPOT) is described, which provides a useful alternative to conventional plaque-forming cell assays.About:
This article is published in Journal of Immunological Methods.The article was published on 1983-12-16. It has received 1047 citations till now. The article focuses on the topics: FluoroSpot & ELISPOT.read more
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Book ChapterDOI
Immunoglobulin A (IgA): molecular and cellular interactions involved in IgA biosynthesis and immune response.
Jiri Mestecky,Jerry R. McGhee +1 more
TL;DR: The impact of new information concerning IgA physiology on the immune system is discussed, which suggests that IgA should not be considered only as an isotype providing specific humoral protection of mucosal surfaces but as an integral component of the entire immune system.
Journal ArticleDOI
Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells.
Cecil Czerkinsky,Gudrun Andersson,Hans-Peter Ekre,Lars-Åke Nilsson,Lars Klareskog,Örjan Ouchterlony +5 more
TL;DR: A reverse modification of ELISPOT assay using nitrocellulose membranes and epitope-specific monoclonal antibodies is described for the detection of single lymphokine-secreting cells.
Journal ArticleDOI
Enzyme-Linked Immunosorbent Assay
TL;DR: Solid-phase immunoassays and ELISA have technically simplified antigen quantitation and antibody detection and are the basis for most modern immunodiagnostic tests for acquired immunodeficiency syndrome (AIDS), hepatitis, allergies, and many other human and animal diseases.
Journal ArticleDOI
Identification of preexisting adaptive immunity to Cas9 proteins in humans
Carsten T. Charlesworth,Priyanka S. Deshpande,Daniel P. Dever,Joab Camarena,Viktor T. Lemgart,M. Kyle Cromer,Christopher A. Vakulskas,Michael A. Collingwood,Liyang Zhang,Nicole M. Bode,Mark A. Behlke,Beruh Dejene,Brandon Cieniewicz,Rosa Romano,Benjamin J. Lesch,Natalia Gomez-Ospina,Sruthi Mantri,Mara Pavel-Dinu,Kenneth I. Weinberg,Matthew H. Porteus +19 more
TL;DR: Together, these data demonstrate that there are preexisting humoral and cell-mediated adaptive immune responses to Cas9 in humans, a finding that should be taken into account as the CRISPR–Cas9 system moves toward clinical trials.
Journal ArticleDOI
Direct access to CD4 + T cells specific for defined antigens according to CD154 expression
Marco Frentsch,Olga Arbach,Dennis Kirchhoff,Beate Moewes,Margitta Worm,Martin Rothe,Alexander Scheffold,Andreas Thiel +7 more
TL;DR: The approach allows assessment of TH cells with a defined specificity for the combined quantitative and qualitative analysis of TH-cell immunity as well as for the isolation of specific TH cells for targeted cellular immunotherapies.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Plaque Formation in Agar by Single Antibody-Producing Cells
Niels K. Jerne,Albert A. Nordin +1 more
TL;DR: Distinct plaques, each of which is due to the release of hemolysin by a single antibody-forming cell, are revealed by complement after incubation, in an agar layer, of a mixture of Sheep red cells and lymphoid cells from a rabbit immunized with sheep red cells.
Journal ArticleDOI
Immunization of dissociated spleen cell cultures from normal mice
TL;DR: There is an increase in the ability to discriminate between different varieties of homologous erythrocytes and the in vitro response does not appear to be limited by whatever mechanisms regulate the in vivo response.
Journal Article
A Modification of the Hemolytic Plaque Assay for Use with Protein Antigens
TL;DR: The method is simple and sensitive and the results mimic the kinetics of the response that is seen in in vivo assays of serum antibody.
Journal ArticleDOI
Use of antibody-coated red cells for the sensitive detection of antigen and in rosette tests for cells bearing surface immunoglobulins.
TL;DR: The chromic chloride method, which is applicable to whole Ig fractions as well as affinity-purified antibodies, is reproducible and has a sensitivity for detection of antigen in the nanogram range.
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A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells
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