Journal ArticleDOI
A human gene that shows identity with the gene encoding the angiotensin receptor is located on chromosome 11
Brian F. O'Dowd,Michael Heiber,Audrey Chan,Henry H.Q. Heng,Lap-Chee Tsui,James L. Kennedy,Xiao-Mei Shi,Arturas Petronis,Susan R. George,Tuan V. Nguyen +9 more
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TLDR
PCR analysis of somatic cell lines found APJ-related sequences to be only present on chromosome 11, and high-resolution mapping by fluorescence in situ hybridization (FISH) sublocalized APJ on band q12.About:
This article is published in Gene.The article was published on 1993-12-22. It has received 769 citations till now. The article focuses on the topics: Gene mapping & Coding region.read more
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Isolation and characterization of a novel endogenous peptide ligand for the human APJ receptor.
Kazuhiko Tatemoto,Masaki Hosoya,Yugo Habata,Ryo Fujii,Tadao Kakegawa,Min-Xu Zou,Yuji Kawamata,Shoji Fukusumi,Shuji Hinuma,Chieko Kitada,Tsutomu Kurokawa,Haruo Onda,Masahiko Fujino +12 more
TL;DR: Synthetic peptides derived from the C-terminal amino acid sequence of bovine preproapelin were capable of specifically promoting the acidification rate in the cells expressing the APJ receptor in a range from 10(-7) to 10(-10) M, indicating that apelin is an endogenous ligand for theAPJ receptor.
Journal ArticleDOI
Apelin, a newly identified adipokine up-regulated by insulin and obesity
Jeremie Boucher,Bernard Masri,Danièle Daviaud,Stephane Gesta,Charlotte Guigné,Anne Mazzucotelli,Isabelle Castan-Laurell,Ivan Tack,Bernard Knibiehler,Christian Carpéné,Yves Audigier,Jean-Sébastien Saulnier-Blache,Philippe Valet +12 more
TL;DR: Apelin is identified as a novel adipocyte endocrine secretion and its potential link with obesity-associated variations of insulin sensitivity status is focused on and evidence that insulin exerts a direct control on apelin gene expression in adipocytes is provided.
Journal ArticleDOI
Characterization of Apelin, the Ligand for the APJ Receptor
Dennis Lee,Regina Cheng,Tuan V. Nguyen,Theresa Fan,Anula Kariyawasam,Yang Liu,Daniel H. Osmond,Susan R. George,Susan R. George,Brian F. O'Dowd,Brian F. O'Dowd +10 more
TL;DR: Both sequence and mRNA expression distribution analyses revealed similarities between apelin and angiotensin II, suggesting they that share related physiological roles.
Journal ArticleDOI
The novel peptide apelin lowers blood pressure via a nitric oxide-dependent mechanism.
Kazuhiko Tatemoto,Kiyoshige Takayama,Min-Xu Zou,Iku Kumaki,Wei Zhang,Kimitsuka Kumano,Mineko Fujimiya +6 more
TL;DR: It was found that mean arterial pressure after the administration of apelin, apelin-13, andapelin-36 at a dose of 10 nmol/kg in anaesthetized rats was reduced by 26+/-5, 11+/-4, and 5-/-4 mm Hg, respectively, suggesting that apelin may lower blood pressure via a nitric oxide-dependent mechanism.
Journal ArticleDOI
Apelin, the Novel Endogenous Ligand of the Orphan Receptor APJ, Regulates Cardiac Contractility
István Szokodi,Pasi Tavi,Gabor Foldes,Sari Voutilainen-Myllylä,Mika Ilves,Heikki Tokola,Sampsa Pikkarainen,Jarkko Piuhola,Jaana Rysä,Miklós Tóth,Heikki Ruskoaho +10 more
TL;DR: The results show that apelin is one of the most potent endogenous positive inotropic substances yet identified and that the inotropic response to apelin may involve activation of PLC, PKC, and sarcolemmal NHE and NCX.
References
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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
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A simple salting out procedure for extracting DNA from human nucleated cells
TL;DR: A rapid, safe and inexpensive method was developed to simplify the deprotein-ization procedure that yielded quantities comparable to those obtained from phenol-chloroform extractions, rendering the entire process of RFLP analysis free of toxic materials.
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Cloning of a delta opioid receptor by functional expression
TL;DR: A complementary DNA clone encoding a functional delta opioid receptor has been identified and shows homology to G protein-coupled receptors, in particular the receptors for somatostatin, angiotensin, and interleukin-8.
Journal ArticleDOI
Sequence alignment of the G-protein coupled receptor superfamily.
TL;DR: This work has compiled and aligned the 74 unique amino acid sequences published to date and review the present understanding of the structural motifs contributing to ligand binding and G-protein coupling.
Journal ArticleDOI
Cloning and expression of a complementary DNA encoding a bovine adrenal angiotensin II type-1 receptor
Kazuyuki Sasaki,Yamano Y,S. Bardhan,Naoharu Iwai,Murray Jj,M. Hasegawa,Y. Matsuda,Tadashi Inagami +7 more
TL;DR: The expression cloning of a complementary DNA encoding a bovine angiotensin II receptor is reported to overcome the difficulties faced in purifying the receptor owing to its instability and low concentration.