Journal ArticleDOI
A method for the determination of protein in the presence of Triton X-100.
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TLDR
The method is applicable to protein assay in the presence of other nonionic detergents under certain conditions and allows the assay of both water-soluble and hydrophobic protein in solutions containing concentrations of Triton X-100 up to at least 4% (v/v).About:
This article is published in Analytical Biochemistry.The article was published on 1979-01-01. It has received 39 citations till now. The article focuses on the topics: Triton X-100 & Bradford protein assay.read more
Citations
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Journal ArticleDOI
A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids
D. Wessel,U.I. Flügge +1 more
TL;DR: A rapid method based on a defined methanol-chloroform-water mixture for the quantitative precipitation of soluble as well as hydrophobic proteins from dilute solutions (e.g., column chromatography effluents) has been developed.
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Review of the Folin phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall.
TL;DR: The method of Lowry and coworkers combined the use of copper, as suggested by Herriott, with the Folin phenol method, which originated from the work of Wu, to produce a more reliable and sensitive protein analysis.
Journal ArticleDOI
Protein contents and physicochemical properties in honey samples of Apis mellifera of different floral origins
TL;DR: In this paper, the protein contents in honey samples of different floral origins, commercialized in several states of Brazil, were determined using the method of Bradford using the spectra of pollen of the honeys collected in those areas, in order to establish the correlation between the different botanical species and protein contents.
Journal ArticleDOI
Purification of extracellular lipase from Pseudomonas aeruginosa.
TL;DR: Characterization of cell-free culture supernatants by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of significant amounts of lipopolysaccharide, part of which seemed to be tightly bound to lipase.
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A rapid and simple method for measurement of total protein in very low density lipoproteins by the Lowry assay.
TL;DR: Addition of 0.1 ml of 2.5% (w/v) sodium dodecyl sulfate to the final reaction mixture is effective in removing turbidity and can be used as an alternative method.
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Determination of serum proteins by means of the biuret reaction.
TL;DR: An investigation of the biochemical changes following experimental liver injury felt the need of a simple, rapid, and accurate method for determining the protein fractions in small amounts of serum and began with Kingsley’s biuret procedure.
Journal ArticleDOI
A simplification of the protein assay method of Lowry et al. which is more generally applicable
TL;DR: A simple method based on a linear log-log protein standard curve is presented to permit rapid and totally objective protein analysis using small programmable calculators.
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Solubilization of membranes by detergents
Ari Helenius,Kai Simons +1 more
TL;DR: This review focuses on work that deals with the mechanisms of detergent action in membrane solubilization including properties of detergents model lipid systems and detergent-protein interactions; in addition a possible sequence of events when deterGents interact with biological membranes receives attention.
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A rapid, sensitive, and specific method for the determination of protein in dilute solution
TL;DR: This protein assay is described in which the sample is precipitated with trichloroacetic acid in the presence of sodium dodecylsulfate, filtered off on a Millipore membrane and stained with Amidoschwarz 10B, and its absorbance determined at 630 nm.