Journal ArticleDOI
A microtransplantation approach for cell suspension grafting in the rat Parkinson model: a detailed account of the methodology.
TLDR
The results demonstrate that single deposits of 50,000-75,000 cells in 0.5 microliter provide the best environment both for dopaminergic and non-dopaminergic neuron survival and Optimal graft integration and minimal disturbances of host brain structures can reliably be achieved by small-sized implants.About:
This article is published in Neuroscience.The article was published on 1994-11-01. It has received 216 citations till now. The article focuses on the topics: Microtransplantation & Dopaminergic.read more
Citations
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Journal ArticleDOI
Cell type analysis of functional fetal dopamine cell suspension transplants in the striatum and substantia nigra of patients with Parkinson's disease
Ivar Mendez,Rosario Sanchez-Pernaute,Oliver Cooper,Angel Viñuela,Daniela Ferrari,Lars Björklund,Alain Dagher,Ole Isacson +7 more
TL;DR: This study demonstrates that, using these methods, dopamine neuronal replacement cell therapy can be beneficial for patients with advanced disease, and that changing technical approaches could have a favourable impact on efficacy and adverse events following neural transplantation.
Book
现代神经科学研究技术 = Modern techniques in neuroscience research
U. Windhorst,H. Johansson,志奇 赵 +2 more
TL;DR: This book discusses techniques at the molecular and single cell level, and at the Organismic Level, as well as strategies for data acquisition, processing and storage management.
Journal ArticleDOI
Improving the survival of grafted dopaminergic neurons: a review over current approaches
Patrik Brundin,Jenny Karlsson,Mia Emgård,Gabriele S. Kaminski Schierle,Oskar Hansson,Åsa Petersén,Roger F. Castilho +6 more
TL;DR: Different approaches that reduce cell death and increase survival of grafted neurons, typically by a factor of 2–4 are reviewed; changes in transplantation procedure such as improved media and implantation technique can be beneficial, and calcium channel antagonists such as nimodipine and flunarizine improve nigral graft survival.
Journal ArticleDOI
GDNF secreting human neural progenitor cells protect dying motor neurons, but not their projection to muscle, in a rat model of familial ALS.
Masatoshi Suzuki,Jacalyn McHugh,Craig Tork,Brandon Shelley,Sandra M. Klein,Patrick Aebischer,Clive N. Svendsen +6 more
TL;DR: The potential to maintain dying motor neurons by delivering GDNF using neural progenitor cells represents a novel and powerful treatment strategy for ALS and is suggested to be the first step of a therapeutic advance for this devastating and incurable disease.
Journal ArticleDOI
GDNF delivery using human neural progenitor cells in a rat model of ALS.
Sandra M. Klein,Soshana Behrstock,Jacalyn McHugh,Kristin Hoffmann,Kyle Wallace,Masatoshi Suzuki,Patrick Aebischer,Clive N. Svendsen +7 more
TL;DR: It is concluded that genetically modified hNPC can survive, integrate, and release GDNF in the spinal cord of SOD1 (G93A) rats, which provide an interesting source of cells for both glial replacement and trophic factor delivery in future human clinical studies.
References
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Journal ArticleDOI
Some new, simple and efficient stereological methods and their use in pathological research and diagnosis.
Hj. Gundersen,Tf. Bendtsen,Lise Korbo,Niels Marcussen,Arne Møller,K. Nielsen,Jr. Nyengaard,Bente Pakkenberg,Flemming Brandt Sørensen,A. Vesterby +9 more
TL;DR: Methods for estimating the volume, surface area and length of any structure are described in this review and the principles on which stereology is based and the necessary sampling procedures are described and illustrated with examples.
Journal ArticleDOI
Quantitative recording of rotational behavior in rats after 6-hydroxy-dopamine lesions of the nigrostriatal dopamine system.
TL;DR: The nigrostriatal dopamine system of rats is unilaterally lesioned by intracerebral injection of 6-hydroxy-dopamine which produces an almost complete and selective lesion and the remaining contralateral dopamine system is activated by systemic treatment with amphetamine.
Journal ArticleDOI
Grafts of fetal dopamine neurons survive and improve motor function in Parkinson's disease
Olle Lindvall,Patrik Brundin,Håkan Widner,S Rehncrona,Björn Gustavii,Richard S. J. Frackowiak,Klaus L. Leenders,G. V. Sawle,John C. Rothwell,C. D. Marsden +9 more
TL;DR: The grafts, which were implanted unilaterally into the putamen by stereotactic surgery, restored dopamine synthesis and storage in the grafted area, as assessed by positron emission tomography with 6-L-[18F]fluorodopa.
Journal ArticleDOI
Bilateral fetal mesencephalic grafting in two patients with parkinsonism induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)
Håkan Widner,J Tetrud,S Rehncrona,B J Snow,Patrik Brundin,Björn Gustavii,Anders Björklund,Olle Lindvall,J W Langston +8 more
TL;DR: Bilateral implantation of fetal mesencephalic tissue can induce substantial long-term functional improvement in patients with parkinsonism and severe dopamine depletion and is accompanied by increased uptake of fluorodopa by the striatum.