Journal ArticleDOI
A new extraction of arginine vasopressin from blood: The use of octadecasilyl-silica
TLDR
Small columns packed with octadecasilylsilica were used to extract arginine-vasopressin in femtomole amounts from biological fluids for radioimmunoassay, effective in isolating the peptide from substances in plasma and serum which interfere with its quantitation.Abstract:
Small columns packed with octadecasilylsilica were used to extract arginine-vasopressin in femtomole amounts from biological fluids for radioimmunoassay. This method is effective in isolating the peptide from substances in plasma and serum which interfere with its quantitation. Consistent and reproducible results and recoveries >80% were obtained with this procedure. High pressure liquid chromatography and serial dilution of extracted samples confirm the identity of the extracted product.read more
Citations
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Journal ArticleDOI
Contribution of vasopressin to vasoconstriction in patients with congestive heart failure: comparison with the renin-angiotensin system and the sympathetic nervous system.
Mark A. Creager,David P. Faxon,Sally S. Cutler,Osvaldo Kohlmann,Thomas J. Ryan,Haralambos Gavras +5 more
TL;DR: Ten patients with advanced congestive heart failure were treated with an arginine vasopressin V1 antagonist during hemodynamic monitoring to determine the contribution of vasoppressin to vasoconstriction in this disorder.
Journal ArticleDOI
Copeptin in the diagnosis of vasopressin-dependent disorders of fluid homeostasis
TL;DR: Copeptin measurements can enable differentiation between various conditions within the polyuria–polydipsia syndrome and the ratio of copeptin to urinary sodium can distinguish the syndrome of inappropriate antidiuretic hormone secretion from other AVP-dependent forms of hyponatraemia.
Journal ArticleDOI
Sexual dimorphism in the posterior pituitary response to stress in the rat.
TL;DR: Data demonstrate that the neural pathways responsible for the neurohypophyseal response to immobilization are not known, and that the response is dependent on the sex of the rat.
Journal ArticleDOI
Role of vasopressin in essential hypertension: racial differences.
TL;DR: AVP seems to play a more important role as a pressor hormone in maintaining the elevation of arterial pressure in African-American hypertensive subjects than it does in Caucasian hypertensives.
Journal ArticleDOI
Central administration of prolactin-releasing peptide stimulates oxytocin release in rats.
Minoru Maruyama,Hirokazu Matsumoto,Ken Fujiwara,Jiro Noguchi,Chieko Kitada,Shuji Hinuma,Haruo Onda,Osamu Nishimura,Masahiko Fujino,Takashi Higuchi,Kinji Inoue +10 more
TL;DR: The results suggest that the PrRP acts as a neuro-modulator of the function of magnocellular neurons, especially oxytocin neurons, in the brain.
References
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Journal ArticleDOI
Development and Clinical Application of a New Method for the Radioimmunoassay of Arginine Vasopressin in Human Plasma
TL;DR: The radioimmunoassay method provides a useful way of assessing AVP function in man and, when used appropriately, approximate the anticipated levels and vary in accordance with physiologic expections.
Journal ArticleDOI
Computer analysis of radioligand assay and radioimmunoassay data.
David Rodbard,J. E. Lewald +1 more
Journal ArticleDOI
Radioimmunoassay measurement of arginine vasopressin in serum: development and application.
TL;DR: The RIA has a sensitivity of 0.3–0.5 μU/ml and employs an extraction procedure utilizing the diatomaceous earth Bentonite that permits AVP measurements in maternal and fetal blood in the presence of elevated levels of vasopressinase.
Journal ArticleDOI
Hydrophobic high-performance liquid chromatography of hormonal polypeptides and proteins on alkylsilane-bonded silica.
M.J. O'Hare,Edouard C. Nice +1 more
TL;DR: The separation of at least eighteen discrete components from a partially-purified posterior pituitary extract has demonstrated the capability of alkylsilane-type reversed-phase packings for the hydrophobic high-performance liquid chromatography of complex biological mixtures.
Journal ArticleDOI
Use of octadecasilyl-silica for the extraction and purification of peptides in biological samples. Application to the identification of circulating metabolites of corticotropin-(1-24)-tetracosapeptide and somatostatin in vivo.
TL;DR: Somatostatin was rapidly cleaved in vivo and in vitro to a single product, which probably retains biological activity and is likely to be inactivated once it leaves the circulation.