Journal ArticleDOI
A possible error during assays for the enzymic phosphorylation of proteins and nucleic acids
TLDR
In this paper, the formation of an insoluble complex between Mg++, sodium fluoride and ATP was studied and the precipitation of ATP was dependent on the time of incubation and on the concentration of ATP, Mg+ and sodium fluoride.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1972-05-12. It has received 29 citations till now. The article focuses on the topics: Sodium & Sodium fluoride.read more
Citations
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Journal ArticleDOI
Regulation of adenosine 3:5-monophosphate-dependent protein kinase.
TL;DR: The results are consistent with the hypothesis that epinephrine and insulin do modify the "activation state" of the cAMP-dependent protein kinase through changes in the camp concentration.
Book ChapterDOI
Chemical properties and separation of phosphoamino acids by thin-layer chromatography and/or electrophoresis.
TL;DR: This chapter discusses the chemical properties and separation of phosphoamino acids by thin-layer chromatography and electrophoresis with respect to intracellular phosphoproteins.
Journal ArticleDOI
Regulation of Adenosine 3',5'-Monophosphate-dependent Protein Kinase I. PRELIMINARY CHARACTERIZATION OF THE ADIPOSE TISSUE ENZYME IN CRUDE EXTRACTS
TL;DR: In this article, the authors investigated the effects of hormones on the adenosine 3',5'-monophosphate (cAMP)-dependent protein kinase of adipose tissue.
Journal ArticleDOI
Occurrence and properties of a chromatin-associated F1-histone phosphokinase in mitotic Chinese hamster cells.
Robert S. Lake,Norman P. Salzman +1 more
Journal ArticleDOI
Endgoenous protein kinase in outer plasma membrane of cultured 3T3 cells. Nature of the membrane-bound substrate and effect of cell density, serum addition, and oncogenic transformation.
A M Mastro,E Rozengurt +1 more
TL;DR: Evidence for the surface location of the kinase was shown by its activity in phosphorlating exogenous substrate, histone, and phosvitin, which increased by 2- to 4-fold prior to the start of S phase when quiescent 3T3 cells were stimulated to reinitiate growth by the addition of serum.
References
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Journal ArticleDOI
A two-dimensional fractionation procedure for radioactive nucleotides
TL;DR: High-voltage ionophoresis is used in both dimensions for the two-dimensional fractionation of ribonuclease digests of 32P-labelled RNA and the determination of the sequence of a nucleotide by partial digestion with spleen phosphodiesterase.
Journal ArticleDOI
Phosphorylation of ribosomal proteins in rabbit reticulocytes. Characterization and regulatory aspects
Journal ArticleDOI
Protein kinase and phosphate acceptor proteins in rauscher murine leukaemia virus
Mette Strand,J. Thomas August +1 more
TL;DR: Rauscher murine leukaemia virus contains both a protein kinase and substrate proteins that seem to include the major structural components of the virus.
Journal ArticleDOI
The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. I. Phosphorylation at 5'-hydroxyl termini.
TL;DR: Evidence was presented that the phosphorylation of RNA and DNA by the purified kinase preparation was catalyzed by the same enzyme, and the ratio ofThese two activities is relatively constant during purification and the rates of inactivation of these two activities at 37° were identical.