Journal ArticleDOI
A primordial and reversible TCA cycle in a facultatively chemolithoautotrophic thermophile
Takuro Nunoura,Yoshito Chikaraishi,Yoshito Chikaraishi,Rikihisa Izaki,Takashi Suwa,Takaaki Sato,Takeshi Harada,Koji Mori,Yumiko Kato,Masayuki Miyazaki,Shigeru Shimamura,Katsunori Yanagawa,Aya Shuto,Naohiko Ohkouchi,Nobuyuki Fujita,Yoshihiro Takaki,Haruyuki Atomi,Ken Takai +17 more
TLDR
Phylometabolic evaluation suggests that the TCA cycle with reversible CS may represent an ancestral mode of the rTCA cycle and raises the possibility of a facultatively chemolithomixotrophic origin of life.Abstract:
Inorganic carbon fixation is essential to sustain life on Earth, and the reductive tricarboxylic acid (rTCA) cycle is one of the most ancient carbon fixation metabolisms A combination of genomic, enzymatic, and metabolomic analyses of a deeply branching chemolithotrophic Thermosulfidibacter takaii ABI70S6 T revealed a previously unknown reversible TCA cycle whose direction was controlled by the available carbon source(s) Under a chemolithoautotrophic condition, a rTCA cycle occurred with the reverse reaction of citrate synthase (CS) and not with the adenosine 5′-triphosphate–dependent citrate cleavage reactions that had been regarded as essential for the conventional rTCA cycle Phylometabolic evaluation suggests that the TCA cycle with reversible CS may represent an ancestral mode of the rTCA cycle and raises the possibility of a facultatively chemolithomixotrophic origin of liferead more
Citations
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Flavin-Based Electron Bifurcation, Ferredoxin, Flavodoxin, and Anaerobic Respiration With Protons (Ech) or NAD+ (Rnf) as Electron Acceptors: A Historical Review.
Wolfgang Buckel,Rudolf K. Thauer +1 more
TL;DR: The electron-bifurcating flavoproteins known to date fall into four groups that have evolved independently, namely those containing EtfAB (CarED, LctCB, FixBA) with bound FAD, a NuoF homolog (HydB, HytB, or HylB) harboring FMN, NfnB with boundFAD, orHdrA harboring FAD.
Journal ArticleDOI
Innovations to culturing the uncultured microbial majority.
TL;DR: Common barriers that can hamper the isolation and culturing of novel microorganisms are discussed and emerging, innovative methods for targeted or high-throughput cultivation are reviewed.
Journal ArticleDOI
Third-generation biorefineries as the means to produce fuels and chemicals from CO2
TL;DR: How microorganisms can be engineered for CO2 fixation and industrial valorization of this key molecule is described, and a shift from sugar-based feedstocks and biomass to the use of atmospheric CO2 for the bioproduction of fuels and chemicals is desirable.
Journal ArticleDOI
Synthesis and breakdown of universal metabolic precursors promoted by iron
TL;DR: A purely chemical reaction network promoted by ferrous iron is described, in which aqueous pyruvate and glyoxylate build up 9 of the 11 intermediates of the biological Krebs (or tricarboxylic acid) cycle, including all 5 universal metabolic precursors.
Journal ArticleDOI
The microbiomes of deep-sea hydrothermal vents: distributed globally, shaped locally.
TL;DR: The challenges and opportunities that vent ecosystems provide for microbial life and their relationship to biogeography are explored, including their relationships with underlying geology and hydrothermal geochemistry.
References
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