A Review on the Adaption of Alginate-Gelatin Hydrogels for 3D Cultures and Bioprinting.
Magdalena Beata Łabowska,Karolina Cierluk,Agnieszka M. Jankowska,Julita Kulbacka,Jerzy Detyna,Izabela Michalak +5 more
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TLDR
An overview of the materials used for 3D cell cultures, which are mainly alginate-gelatin hydrogels, including their properties and potential applications, can be found in this paper.Abstract:
Sustaining the vital functions of cells outside the organism requires strictly defined parameters In order to ensure their optimal growth and development, it is necessary to provide a range of nutrients and regulators Hydrogels are excellent materials for 3D in vitro cell cultures Their ability to retain large amounts of liquid, as well as their biocompatibility, soft structures, and mechanical properties similar to these of living tissues, provide appropriate microenvironments that mimic extracellular matrix functions The wide range of natural and synthetic polymeric materials, as well as the simplicity of their physico-chemical modification, allow the mechanical properties to be adjusted for different requirements Sodium alginate-based hydrogel is a frequently used material for cell culture The lack of cell-interactive properties makes this polysaccharide the most often applied in combination with other materials, including gelatin The combination of both materials increases their biological activity and improves their material properties, making this combination a frequently used material in 3D printing technology The use of hydrogels as inks in 3D printing allows the accurate manufacturing of scaffolds with complex shapes and geometries The aim of this paper is to provide an overview of the materials used for 3D cell cultures, which are mainly alginate–gelatin hydrogels, including their properties and potential applicationsread more
Citations
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3D printing of alginate dialdehyde-gelatin (ADA-GEL) hydrogels incorporating phytotherapeutic icariin loaded mesoporous SiO2-CaO nanoparticles for bone tissue engineering
Mahshid Monavari,Shahin Homaeigohar,Miguel Fuentes-Chandía,Qaisar Nawaz,Mehran Monavari,Arvind Venkatraman,Aldo R. Boccaccini +6 more
TL;DR: 3D printing enables a better control over the microstructure of bone restoring constructs, addresses the challenges seen in the preparation of patient-specific bone scaffolds, and overcomes the bottlenecks that can appear in delivering drugs/growth factors promoting bone regeneration.
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Physico-chemical modification of gelatine for the improvement of 3D printability of oxidized alginate-gelatine hydrogels towards cartilage tissue engineering
TL;DR: The possibility to enhance the printability of ADA-GEL hydrogels to fabricate hierarchical scaffold structures with shape stability and fidelity, without the necessity to change the initial hydrogel chemistry by the use of additives or crosslinkers is demonstrated.
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Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.
Zhiwei Liao,Hui Liu,Liang Ma,Jie Lei,Bide Tong,Gaocai Li,Wencan Ke,Kun Wang,Xiaobo Feng,Wenbin Hua,Shuai Li,Cao Yang +11 more
TL;DR: In this article, Cavin-2-modified extracellular vesicles (EVs) were used to protect against pyroptosis of nucleus pulposus cells (NPCs).
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3D printing of bio-instructive materials: Toward directing the cell
TL;DR: In this paper , extrusion-based 3D printing methods (EBP) are evaluated in their ability to direct cell alignment, proliferation, differentiation, specific ECM production, and tissue maturation.
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3D bioprinted drug-resistant breast cancer spheroids for quantitative in situ evaluation of drug resistance
TL;DR: In this paper , a 3D bioprinting-based drug screening model was proposed to evaluate the efficacy of anticancer drugs using drug-resistant MCF-7 breast cancer spheroids formed within a printed hydrogel.
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