Journal ArticleDOI
A Role for the AKT1 Potassium Channel in Plant Nutrition
Reads0
Chats0
TLDR
Results and membrane potential measurements suggest that the AKT1 channel mediates potassium uptake from solutions that contain as little as 10 micromolar potassium.Abstract:
In plants, potassium serves an essential role as an osmoticum and charge carrier. Its uptake by roots occurs by poorly defined mechanisms. To determine the role of potassium channels in planta, we performed a reverse genetic screen and identified an Arabidopsis thaliana mutant in which the AKT1 channel gene was disrupted. Roots of this mutant lacked inward-rectifying potassium channels and displayed reduced potassium (rubidium-86) uptake. Compared with wild type, mutant plants grew poorly on media with a potassium concentration of 100 micromolar or less. These results and membrane potential measurements suggest that the AKT1 channel mediates potassium uptake from solutions that contain as little as 10 micromolar potassium.read more
Citations
More filters
Journal ArticleDOI
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
Steven J. Clough,Andrew F. Bent +1 more
TL;DR: The modified method should facilitate high-throughput transformation of Arabidopsis for efforts such as T-DNA gene tagging, positional cloning, or attempts at targeted gene replacement.
Journal ArticleDOI
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
TL;DR: This is the first complete genome sequence of a plant and provides the foundations for more comprehensive comparison of conserved processes in all eukaryotes, identifying a wide range of plant-specific gene functions and establishing rapid systematic ways to identify genes for crop improvement.
Journal ArticleDOI
Plant cellular and molecular responses to high salinity.
TL;DR: Evidence for plant stress signaling systems is summarized, some of which have components analogous to those that regulate osmotic stress responses of yeast, some that presumably function in intercellular coordination or regulation of effector genes in a cell-/tissue-specific context required for tolerance of plants.
Journal ArticleDOI
Reactive oxygen species produced by nadph oxidase regulate plant cell growth
Julia Foreman,Vadim Demidchik,John H. F. Bothwell,Panagiota Mylona,Henk Miedema,Miguel Angel Torres,Miguel Angel Torres,Paul Linstead,Silvia Costa,Colin Brownlee,Jonathan D. G. Jones,Julia M. Davies,Liam Dolan +12 more
TL;DR: It is shown here that RHD2 is an NADPH oxidase, a protein that transfers electrons from NADPH to an electron acceptor leading to the formation of reactive oxygen species (ROS) and that ROS accumulate in growing wild-type (WT) root hairs but their levels are markedly decreased in rhd2 mutants.
Journal ArticleDOI
Geometric and Electronic Structure/Function Correlations in Non-Heme Iron Enzymes
Edward I. Solomon,Thomas C. Brunold,Mindy I. Davis,Jyllian N. Kemsley,Sang-Kyu Lee,Nicolai Lehnert,Frank Neese,Andrew J. Skulan,Yi-Shan Yang,Jing Zhou +9 more
TL;DR: A detailed molecular mechanism has been proposed for IPNS based on spectroscopic and crystallographic studies and the role of cosubstrate ascorbate is proposed to reduce the toxic peroxo byproduct to water.
References
More filters
Journal ArticleDOI
Cloning and expression in yeast of a plant potassium ion transport system.
Hervé Sentenac,Nathalie Bonneaud,Michèle Minet,François Lacroute,Jean-Michel Salmon,Frédéric Gaymard,Claude Grignon +6 more
TL;DR: A membrane polypeptide involved in K+ transport in a higher plant was cloned by complementation of a yeast mutant defective in K+, with a complementary DNA library from Arabidopsis thaliana that conferred ability to grow on media with K+ concentration in the micromolar range and to absorb K+ (or 86Rb+) at rates similar to those in wild-type yeast.
Journal ArticleDOI
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
TL;DR: The isolation of a cDNA from Arabidopsis thaliana that encodes a probable K+ channel and the results suggest that the structural motif for K+ channels has been conserved between plants and animals.
Journal ArticleDOI
Structure and transport mechanism of a high-affinity potassium uptake transporter from higher plants
TL;DR: This work uses expression cloning to isolate a complementary DNA encoding a membrane protein (HKT1) from wheat roots which confers the ability to take up K+.
Journal ArticleDOI
Resolution of dual mechanisms of potassium absorption by barley roots
TL;DR: The relationship between the rates of absorption of K and Rb by barley roots and the concentration of these ions in the external solution, over the range 0.002 to 50 mM, is predictable on the assumption that two carrier sites bind and transport the ions.
Journal ArticleDOI
Potassium homeostasis in vacuolate plant cells
TL;DR: Measurements of the changes in K+ activity in the vacuole and cytosol of barley root cells revealed that vacuolar aK declined linearly with decreases in tissue K+ concentration, whereas cytosolic aK initially remained constant in both epidermal and cortical cells but then declined at different rates in each cell type.