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A Structurally Self-Assembled Peptide Nano-Architecture by One-Step Electrospinning

AbstractSelf-assembling peptides (SAPs) have shown to offer great promise in therapeutics and have the ability to undergo self-assembly and form ordered nanostructures. However SAP gels are often associated with inherent weak and transient mechanical properties and incorporation of them into polymeric matrices is a route to enhance their mechanical stability. The aim of this work was to incorporate P11-8 peptide (CH3COQQRFOWOFEQQNH2) within poly(epsilon-caprolactone) (PCL) fibrous webs via one-step electrospinning, aiming to establish the underlying relationships between spinning process, molecular peptide conformation, and material internal architecture. Electrospinning of PCL solutions (6% w/w) in hexafluoro-2-propanol (HFIP) containing up to 40 mg/ml P11-8 resulted in the formation of fibres in both nano- (10-100 nm) and submicron range (100-700 nm), in contrast to PCL only webs, which displayed a predominantly submicron fibre distribution. FTIR and CD spectroscopy on both PCL/peptide solutions and resulting electrospun webs revealed monomeric and beta-sheet secondary conformation, respectively, suggesting the occurrence of peptide self-assembly during electrospinning due to solvent evaporation. The peptide concentration (0 -> 40 mg/ml) was found to primarily affect the internal structure of the fabric at the nano-scale, whilst water as well as cell culture medium contact angles were dramatically decreased. Nearly no cytotoxic response (> 90% cell viability) was observed when L929 mouse fibroblasts were cultured in contact with electrospun peptide loaded samples. This novel nanofibrous architecture may be the basis for an interesting material platform for e.g. hard tissue repair, in light of the presence of the self assembled P11-8 in the PCL fibrous structure.

Topics: Peptide Conformation (55%), Electrospinning (55%)

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This is a repository copy of A structurally self-assembled peptide nano-architecture by
one-step electrospinning.
White Rose Research Online URL for this paper:
http://eprints.whiterose.ac.uk/102894/
Version: Accepted Version
Article:
Gharaei, R orcid.org/0000-0002-9698-6649, Tronci, G, Davies, RPW et al. (4 more
authors) (2016) A structurally self-assembled peptide nano-architecture by one-step
electrospinning. Journal of Materials Chemistry B, 4 (32). pp. 5475-5485. ISSN 2050-750X
https://doi.org/10.1039/C6TB01164K
© 2016 The Royal Society of Chemistry. This is an author produced version of a paper
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Robabeh Gharaei,
†,§
Giuseppe Tronci,
†,‡
Robert P.W. Davies,
Caroline Gough,
Reem Alazragi,
Parikshit Goswami,
§
and Stephen J. Russell
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Page 1 of 27 Journal of Materials Chemistry B
Journal of Materials Chemistry B Accepted Manuscript
Published on 25 July 2016. Downloaded by University of Leeds on 25/07/2016 13:33:14.
View Article Online
DOI: 10.1039/C6TB01164K

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*I= #/"G""."5B
Page 2 of 27Journal of Materials Chemistry B
Journal of Materials Chemistry B Accepted Manuscript
Published on 25 July 2016. Downloaded by University of Leeds on 25/07/2016 13:33:14.
View Article Online
DOI: 10.1039/C6TB01164K

;

                
              /
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    B B 7568  "    !# 
J"
&
/
;"K"?
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
J"
/
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L8      
G7
98"798"7987
8"B/
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G"
/)1
2
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:
         
/"
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"
 /
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
 / 1"  "   
J       9   /) 
9Bin vivo"
  /       
"O
Page 3 of 27 Journal of Materials Chemistry B
Journal of Materials Chemistry B Accepted Manuscript
Published on 25 July 2016. Downloaded by University of Leeds on 25/07/2016 13:33:14.
View Article Online
DOI: 10.1039/C6TB01164K

D
/
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          "    
/
    J      " 
99,';E/
;

               
          /
D
)B/
;"K:
C
        56
'
"     
Bin vivoin vitro/
?


"
K"?"&E&?

  J          
/
&2" &:
.
7!8"
&'
7%8
;E";

5%/
;&" ;;
 " 5%     ,#   //    
        /
;&" ;D
5%  " B 
/5%B
    "   "
&;" &K" ;K;2

        "   " 
/
;&" ;:DE
1"    9   J   
9"5%//A/
;&"
D"D&

C   "    78  
B  "       /
Page 4 of 27Journal of Materials Chemistry B
Journal of Materials Chemistry B Accepted Manuscript
Published on 25 July 2016. Downloaded by University of Leeds on 25/07/2016 13:33:14.
View Article Online
DOI: 10.1039/C6TB01164K

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01 Jan 2014
TL;DR: The book highlights the key features which enable engineers to design stimuli-responsive smart nanoparticles, novel biomaterials, nano/micro- devices for diagnosis, therapy (theranostics), and up and coming bio-engineering devices.
Abstract: Advanced materials are attracting strong interest in the fundamental as well as applied sciences and are being extensively explored for their potential usage in a range of healthcare technological ...

136 citations


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