Journal ArticleDOI
Acute effects of glyphosate herbicide on metabolic and enzymatic parameters of silver catfish (Rhamdia quelen).
Lissandra Glusczak,Denise dos Santos Miron,Bibiana Silveira Moraes,Róli Rodrigues Simões,Maria Rosa Chitolina Schetinger,Vera Maria Morsch,Vânia Lúcia Loro +6 more
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TLDR
The present results showed that in 96 h, glyphosate changed AChE activity, metabolic parameters and TBARS production, which can be used as herbicide toxicity indicators considering environmentally relevant concentration.Abstract:
Silver catfish ( Rhamdia quelen ; Teleostei) were exposed to commercial formulation Roundup®, a glyphosate herbicide: 0 (control), 0.2 or 0.4 mg/L for 96 h. Fish exposed to glyphosate showed an increase in hepatic glycogen, but a reduction in muscle glycogen at both concentrations tested. Glucose decreased in liver and increased in muscle of fish at both herbicide concentrations. Glyphosate exposure increased lactate levels in liver and white muscle at both concentrations. Protein levels increased in liver and decreased in white muscle while levels of ammonia in both tissues increased in fish at both glyphosate concentrations. Specific AChE activity was reduced in brain after treatments, no changes were observed in muscle tissue. Catalase activity in liver did not change during of exposure. Fish exposed to glyphosate demonstrated increased TBARS production in muscle tissue at both concentrations tested. For both glyphosate concentrations tested brain showed a reduction of TBARS after 96 h of exposure. The present results showed that in 96 h, glyphosate changed AChE activity, metabolic parameters and TBARS production. The parameters measured can be used as herbicide toxicity indicators considering environmentally relevant concentration.read more
Citations
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Journal ArticleDOI
Environmentally induced oxidative stress in aquatic animals
TL;DR: The general processes responsible for ROS generation in aquatic animals are described and the identification of its general characteristics and mechanisms responsible for adaptation to the stress have been discussed.
Journal ArticleDOI
Potential toxic effects of glyphosate and its commercial formulations below regulatory limits.
TL;DR: A coherent body of evidence is revealed indicating that GlyBH could be toxic below the regulatory lowest observed adverse effect level for chronic toxic effects, which includes teratogenic, tumorigenic and hepatorenal effects.
Journal ArticleDOI
Impact of glyphosate and glyphosate‐based herbicides on the freshwater environment
TL;DR: This review is designed to update previous reviews of glyphosate‐based herbicide toxicity, with a focus on recent studies of the aquatic toxicity of this class of chemicals.
Journal ArticleDOI
Roundup causes oxidative stress in liver and inhibits acetylcholinesterase in muscle and brain of the fish Prochilodus lineatus.
TL;DR: Inhibition of AChE showed that RD acts as a contaminant with anti-AChE action, which stimulated the biotransformation pathway, with increased GST, but interfered on the antioxidant defenses, with reduction of SOD and GPx activity, leading to the occurrence of lipid peroxidation.
Journal ArticleDOI
Toxicity and effects of a glyphosate-based herbicide on the Neotropical fish Prochilodus lineatus.
TL;DR: Short-term exposure to Roundup at subletal concentrations induced biochemical, physiological and histological alterations in P. lineatus which should be considered when establishing criteria for water quality and animal well-being in the Neotropical region.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction
TL;DR: Using this method, the liped peroxide level in the liver of rats suffering from carbon tetrachloride intoxication was investigated and was in good agreement with previously reported data obtained by measuring diene content.
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A new and rapid colorimetric determination of acetylcholinesterase activity.
TL;DR: A photometric method for determining acetylcholinesterase activity of tissue extracts, homogenates, cell suspensions, etc., has been described and Kinetic constants determined by this system for erythrocyte eholinesterases are presented.