Adipose-derived stem cells: isolation, expansion and differentiation.
TLDR
Methods for the isolation, expansion and differentiation of ASCs are presented and described in detail and can be applied to adipose tissues from other species with minimal modifications.About:
This article is published in Methods.The article was published on 2008-06-01 and is currently open access. It has received 965 citations till now. The article focuses on the topics: Stem cell transplantation for articular cartilage repair & Adipose tissue.read more
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Bone Tissue Engineering: Recent Advances and Challenges
TL;DR: The fundamentals of bone tissue engineering are discussed, highlighting the current state of this field, and the recent advances of biomaterial and cell-based research, as well as approaches used to enhance bone regeneration.
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A nonviral minicircle vector for deriving human iPS cells
Fangjun Jia,Kitchener D. Wilson,Ning Sun,Deepak M. Gupta,Mei Huang,Zongjin Li,Nicholas J. Panetta,Zhi-Ying Chen,Robert C. Robbins,Mark A. Kay,Michael T. Longaker,Joseph C. Wu +11 more
TL;DR: 'minicircle'
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Sox9 Modulates Proliferation and Expression of Osteogenic Markers of Adipose-Derived Stem Cells (ASC)
TL;DR: A pro-proliferative role for Sox9 in undifferentiated rASC is suggested which may explain the higher proliferation rate of rASC compared to rBMSC and an osteogenic differentiation delaying role of Sox9 is proposed which suggests that Sox9 expression is needed to maintain rASC in an undifferentiate, proliferative state.
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Repair of Tissues by Adult Stem/Progenitor Cells (MSCs): Controversies, Myths, and Changing Paradigms
TL;DR: The results have generated several controversies, a few myths and a change in a major paradigm, and how they can be applied to the adult stem/progenitor cells from bone marrow, referred to as MSCs are reviewed.
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Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells.
Ning Sun,Nicholas J. Panetta,Deepak M. Gupta,Kitchener D. Wilson,Andrew S. Lee,Fangjun Jia,Shijun Hu,Athena M. Cherry,Robert C. Robbins,Michael T. Longaker,Joseph C. Wu +10 more
TL;DR: It is shown here that induced pluripotent stem cells can be generated from adult human adipose stem cells (hASCs) freshly isolated from patients, and can be readily derived from adult hASCs in a feeder-free condition, thereby eliminating potential variability caused by using feeder cells.
References
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Multilineage Potential of Adult Human Mesenchymal Stem Cells
Mark F. Pittenger,Alastair Morgan Mackay,Stephen C. Beck,Rama K. Jaiswal,Robin Douglas,Joseph D. Mosca,Mark Aaron Moorman,Donald William Jr. Ward Road Simonetti,Stewart Craig,Daniel R. Marshak +9 more
TL;DR: Adult stem cells isolated from marrow aspirates of volunteer donors could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages.
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Human Adipose Tissue Is a Source of Multipotent Stem Cells
Patricia A. Zuk,Min Zhu,Peter Ashjian,Daniel A. De Ugarte,Jerry I. Huang,Hiroshi Mizuno,Zeni Alfonso,John K. Fraser,Prosper Benhaim,Marc H. Hedrick +9 more
TL;DR: To confirm whether adipose tissue contains stem cells, the PLA population and multiple clonal isolates were analyzed using several molecular and biochemical approaches and PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression.
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Marrow Stromal Cells as Stem Cells for Nonhematopoietic Tissues
TL;DR: Marrow stromal cells present an intriguing model for examining the differentiation of stem cells and have several characteristics that make them potentially useful for cell and gene therapy.
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Bone marrow as a potential source of hepatic oval cells.
Bryon E. Petersen,W. C. Bowen,K. D. Patrene,Wendy M. Mars,A. K. Sullivan,N. Murase,S. S. Boggs,Joel S. Greenberger,Julie P. Goff +8 more
TL;DR: A stem cell associated with the bone marrow has epithelial cell lineage capability and a proportion of the regenerated hepatic cells were shown to be donor-derived.
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Dystrophin expression in the mdx mouse restored by stem cell transplantation
Emanuela Gussoni,Yuko Soneoka,Corinne D. Strickland,Elizabeth A. Buzney,Mohamed K. Khan,Alan F. Flint,Louis M. Kunkel,Louis M. Kunkel,Richard C. Mulligan +8 more
TL;DR: Results suggest that the transplantation of different stem cell populations, using the procedures of bone marrow transplantation, might provide an unanticipated avenue for treating muscular dystrophy as well as other diseases where the systemic delivery of therapeutic cells to sites throughout the body is critical.