Journal ArticleDOI
Aggregation kinetics of bovine serum albumin studied by FTIR spectroscopy and light scattering.
Valeria Militello,Carlo Casarino,Antonio Emanuele,Antonella Giostra,Filippo Pullara,Maurizio Leone +5 more
TLDR
A general mechanism of aggregation composed by partial unfolding of the tertiary structure and by the decrease of alpha-helix and random coil contents in favor of beta-sheet aggregates is indicated for BSA.About:
This article is published in Biophysical Chemistry.The article was published on 2003-11-04. It has received 279 citations till now. The article focuses on the topics: Protein aggregation & Static light scattering.read more
Citations
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Journal ArticleDOI
Photoluminescent diamond nanoparticles for cell labeling: study of the uptake mechanism in mammalian cells.
Orestis Faklaris,Vandana Joshi,Theano Irinopoulou,Patrick Tauc,Mohamed Sennour,Hugues A. Girard,Céline Gesset,Jean-Charles Arnault,Alain Thorel,Jean-Paul Boudou,Patrick A. Curmi,François Treussart +11 more
TL;DR: It is shown that nanodiamonds enter cells mainly by endocytosis, and converging data indicate that it is clathrin-mediated, and the results pave the way for the use of photoluminescent nanod diamonds in targeted intracellular labeling or biomolecule delivery.
Journal ArticleDOI
Quantitative and Qualitative Evaluation of Adsorption/Desorption of Bovine Serum Albumin on Hydrophilic and Hydrophobic Surfaces
TL;DR: The formation of BSA-phosphate surface complexes was observed under different BSA adsorption conditions on hydrophobic and hydrophilic surfaces, correlated with the more efficient blocking of nonspecific interactions by the adsorbed BSA layer.
Journal ArticleDOI
Photoluminescent diamond nanoparticles for cell labeling: study of the uptake mechanism in mammalian cells
Orestis Faklaris,Vandana Joshi,Theano Irinopoulou,Patrick Tauc,Hugues A. Girard,Céline Gesset,Mohamed Senour,Alain Thorel,Jean-Charles Arnault,Jean-Paul Boudou,Patrick A. Curmi,François Treussart +11 more
TL;DR: In this article, the authors used photoluminescent nanodiamonds of size <50 nm for intracellular labeling and investigated the mechanism of their uptake by living cells, by blocking selectively different uptake processes and converging data indicate that it is clathrin mediated.
Journal ArticleDOI
The α-Helix to β-Sheet Transition in Stretched and Compressed Hydrated Fibrin Clots
TL;DR: It is concluded that under extension and/or compression an α-helix to β-sheet conversion of the coiled-coils occurs in the fibrin clot as a part of forced protein unfolding.
Journal ArticleDOI
Protein structural perturbation and aggregation on homogeneous surfaces.
TL;DR: The results presented in this article demonstrate the utility of the ATR/FTIR spectroscopic technique for in situ characterization of protein secondary structures during adsorption on flat surfaces.
References
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Journal ArticleDOI
Dominant forces in protein folding
TL;DR: The present review aims to provide a reassessment of the factors important for folding in light of current knowledge, including contributions to the free energy of folding arising from electrostatics, hydrogen-bonding and van der Waals interactions, intrinsic propensities, and hydrophobic interactions.
Book ChapterDOI
Structure of serum albumin.
Daniel C. Carter,Joseph X. Ho +1 more
TL;DR: This chapter provides an insight of the findings of past significant papers with the current knowledge of the recently determined high resolution X-ray structure of serum albumin and suggests that AFP may have a higher affinity for some unknown ligands important for fetal development.
Journal ArticleDOI
Examination of the secondary structure of proteins by deconvolved FTIR spectra.
D M Byler,H. Susi +1 more
TL;DR: Fourier transform ir (FTIR) spectra of 21 globular proteins have been obtained, revealing that the amide I band of each protein except casein consists of six to nine components, although all proteins do not exhibit components at every characteristic frequency.
Journal ArticleDOI
Analysis of Macromolecular Polydispersity in Intensity Correlation Spectroscopy: The Method of Cumulants
TL;DR: The first order electric field correlation function of laser light scattered by polydisperse solutions of macromolecules can be written as a sum or distribution of exponentials, with decay rates proportional to the diffusion coefficients of the solute molecules as discussed by the authors.