Journal ArticleDOI
An improved colour reagent for the determination of blood glucose by the oxidase system
Denise Barham,P. Trinder +1 more
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A method involving the use of 4-aminophenazone as a colour coupler with sulphonated 2,4-dichlorophenol is described for determining the hydrogen peroxide produced from glucose with glucose oxidase.Abstract:
A method involving the use of 4-aminophenazone as a colour coupler with sulphonated 2,4-dichlorophenol is described for determining the hydrogen peroxide produced from glucose with glucose oxidase.The sensitivity of the method is such that 20 µg of glucose in a final volume of 4 ml give an optical density of 0·61 at 515 nm with 10-mm cells, corresponding to a molecular absorption of 22 000.read more
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Journal ArticleDOI
Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.
TL;DR: A new direct colorimetric procedure for uric acid assay in serum or urine is described, utilizing a 3,5-dichloro-2-hydroxybenzene sulfonic acid/4-aminophenazone chromogenic system in the presence of horseradish peroxidase and uricase from Aspergillus flavus, affording useful results with sample/reagent volume ratios as low as 0.025.
Journal ArticleDOI
Antidiabetic effect of garlic (Allium sativum L.) in normal and streptozotocin-induced diabetic rats.
TL;DR: The antidiabetic effect of garlic ethanolic extract was more effective than that observed with glibenclamide and the plant must be considered as excellent candidate for future studies on diabetes mellitus.
Journal ArticleDOI
Main Risk Factors for Nephropathy in Type 2 Diabetes Mellitus Are Plasma Cholesterol Levels, Mean Blood Pressure, and Hyperglycemia
TL;DR: The combination of blood pressure values in the high-normal range with moderately elevated levels of total cholesterol and hemoglobin A1c defines a high-risk group for the progression to diabetic nephropathy and for clinical events related to arteriosclerotic cardiovascular disease.
Journal ArticleDOI
A sensitive and versatile chromogenic assay for peroxidase and peroxidase-coupled reactions.
That T. Ngo,Howard M. Lenhoff +1 more
TL;DR: Comparing this assay to others shows it to have many more advantages; for example, in addition to its increased sensitivity and versatility, it employs compounds not shown to be carcinogenic and that are very soluble in water.
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