Annonaa muricata Linn Leaf Induce Apoptosis in Cancer Cause Virus
Okid Parama Astirin,Anief Nur Artanti,Meutia Srikandi Fitria,Eva Agustina Perwitasari,Adi Prayitno +4 more
TLDR
Annona muricata Linn leaf in chloroform is a good candidate for chemoprevention escort chemotherapy for cancer causing viruses.Abstract:
Introduction: Now many studies conducted on the drug substance from nature that can serve as an anticancer agent as a potential chemoprevention agent, such as Annona muricata Linn leaf escort chemotherapy, which was flaring. The cancer cell in humans was included the loss of p53 protein function due to mutations in the protein gene. Other causes are that the p53 proteins are not functioning due to an increase in protein misfolding event chaperones and degradation events ubiquitous as binding by viral protein. Method: Cytotoxicity assay performed on 24 well plate micro-cultures. HeLa cells are as 2 × 10 4 cells in 100 mL in RPMI media. Created control is RPMI and solvent DMSO 0.25%. Cytotoxic Test performed by the method of calculation tryphan blue dye exclusion. Being fasted for 24 hours in the culture medium, then the cells are grown in micro-plate with media plus samples with a non-lethal concentration (LC50) of partition and fractionation Annona muricata Linn leaf. Sampling is performed at 24 hours. Each of these wells is calculated the number of living cells and made the curve of cell number and incubation time. Result: The results showed that HeLa cells are being LC50 partition of leaves Annona muricata Linn in ethyl acetate his cell death rate was higher (2000 µg/ml have 131.89%; 15.625 µg/ml have 11.37%) and in ethanol-distillate water his cell death rate was lower (2000 µg/ml have 35.80%; 15.625 µg/ml have 3.97%). Another results showed that HeLa cells are being LC50 fractionation of leaves Annona muricata Linn in chloroform his cell death rate was higher (2000 µg/ml have 91.86%; 15.625 µg/ml have 2.68%) and in ethyl acetate, his cell death rate was lower (2000 µg/ml have 23.79%; 15.625 µg/ml have 4.69%). Figure regression LC50 of HeLa cell culture treatment with partition or fractionation looks of regression test is the positive regression coefficient. Conclusion: Annona muricata Linn leaf in chloroform is a good candidate for chemoprevention escort chemotherapy for cancer causing viruses.read more
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Annona muricata (Annonaceae): A Review of Its Traditional Uses, Isolated Acetogenins and Biological Activities
Soheil Zorofchian Moghadamtousi,Mehran Fadaeinasab,Sonia Nikzad,Gokula Mohan,Hapipah Mohd Ali,Habsah Abdul Kadir +5 more
TL;DR: Phytochemical studies reveal that annonaceous acetogenins are the major constituents of A. muricata, and indigenous communities in Africa and South America extensively use this plant in their folk medicine.
Journal ArticleDOI
Annona muricata: A comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity
TL;DR: This review focuses on the phytochemicals contents, bioactivity, biological actions and toxicological aspects of extracts and isolated compounds, as well as medicinal uses of A. muricata, with the objective of stimulating further studies on extracts and fruit pulp used for human consumption.
Journal ArticleDOI
Anticancer Properties of Graviola (Annona muricata): A Comprehensive Mechanistic Review
Islam Rady,Melissa B. Bloch,Roxane Cherille N. Chamcheu,Sergette Banang Mbeumi,Rafi Anwar,Hadir Mohamed,AS Babatunde,Jules-Roger Kuiate,Felicite K. Noubissi,Khalid A. El Sayed,G. Kerr Whitfield,Jean Christopher Chamcheu,Jean Christopher Chamcheu +12 more
TL;DR: This review summarizes the current understanding of the anticancer effects of A. muricata and its constituents on diverse cancer types and disease states, as well as efficacy and safety concerns.
Journal Article
Targeting apoptosis pathways in cancer therapy
TL;DR: Understanding the molecular mechanisms that regulate cell death programs including apoptosis, and how resistant forms of cancer evade apoptotic events, may provide novel opportunities for cancer drug development.
Journal ArticleDOI
Antiproliferative activity of aqueous leaf extract of Annona muricata L. on the prostate, BPH-1 cells, and some target genes.
George A. Asare,Dan K Afriyie,Robert A. Ngala,Harry Abutiate,Derek Doku,Seidu A. Mahmood,Habibur Rahman +6 more
TL;DR: Prostate of test groups were reduced with epithelial lining showing pyknotic nucleus, condensation, and marginalization of the nuclear material, characteristic of apoptosis of the glandular epithelium, possibly through apoptosis.
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Brine shrimp: a convenient general bioassay for active plant constituents.
Brian N. Meyer,Nelson R. Ferrigni,J. E. Putnam,Linda B. Jacobsen,David E. Nichols,Jerry L. McLaughlin +5 more
TL;DR: Screening results with seed extracts of 41 species of Euphorbiaceae were compared with 9KB and 9PS cytotoxicities and the method is rapid, reliable, inexpensive, and convenient as an in-house general bioassay tool.
A convenient general bioassay for active plant constituents
Brian N. Meyer,Nelson R. Ferrigni,J. E. Putnam,J B Jacobson,D E Nicholas,Jerry L. McLaughlin,B Shrimp +6 more
TL;DR: In this article, a simple bioassay for natural product research using brine shrimp (Artemia salina leach) is proposed, which determines LC (50) values in microg/ml of active compounds and extracts in the brine medium.
Journal ArticleDOI
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Matthias P. Mayer,Bernd Bukau +1 more
TL;DR: This work has shown that for specific tasks the Hsp70 cycle is coupled to the action of other chaperones, such as Hsp90 and Hsp100, and this ATPase cycle is controlled by co-chaperones of the family of J-domain proteins, which target H Sp70s to their substrates, and by nucleotide exchange factors, which determine the lifetime of the HSp70-substrate complex.