Anti-Inflammatory evaluation of black rice extract inhibits TNF-α, IFN-γ and IL-6 cytokines produced by immunocompetent cells

TLDR: In this paper, the anti-inflammatory activity of ethanol extract and aqueous extract of black rice was compared in vitro on splenocytes of diabetes mellitus mice model as the control of inflammation Cells were cultured in Roswell Park Memorial Institute medium with 10% fetal bovine serum, anti-CD3 and lipopolysaccharide and black rice extract 50, 100 and 200

Abstract: Black rice has been known for its many beneficial effects to our body because it contains several chemical compounds that act as anti-inflammatory agents This study aims to compare the anti-inflammatory activity of ethanol extract and aqueous extract of black rice Anti-inflammatory activity test was performed in vitro on splenocytes of diabetes mellitus mice model as the control of inflammation Cells were cultured in Roswell Park Memorial Institute medium with 10% fetal bovine serum, anti-CD3 and lipopolysaccharide and black rice extract 50, 100 and 200 µg/mL In day 3, cells were harvested, stained and analyzed by flow cytometry The result shows that the ethanol extract and aqueous extract of black rice have anti-inflammatory activity 50 µg/mL black rice aqueous extract has the most extensive anti-inflammatory activity indicated by increased Treg cells, decreased nuclear factor kappa B activity on CD4+, CD8+ T cells, decreased production of TNF-α by CD4+ T cells, decreased production of IL-6