Antigenicity of the Leishmania infantum histones H2B and H4 during canine viscerocutaneous leishmaniasis
Manuel Soto,Jose M. Requena,Luis Quijada,M J Perez,C.G. Nieto,Fanny Guzmán,Manuel E. Patarroyo,Carlos Alonso +7 more
TLDR
It is shown that sera from dogs naturally infected with Leishmania infantum contain antibodies that specifically react against the parasite H2B and H4 histones, and the main linear antigenic determinants are located in the amino‐terminal domains of these histones.Abstract:
In this study we show that sera from dogs naturally infected with Leishmania infantum contain antibodies that specifically react against the parasite H2B and H4 histones. The Leishmania H2B and the amino-terminal region of the histone H4, expressed as fusion proteins, when confronted with sera from canine viscerocutaneous leishmaniasis (VCL) dogs, were recognized by 63% and 47%, respectively. No reactivity was detected when sera from dogs naturally infected with pathogens other than Leishmania were used. Using a collection of synthetic peptides covering the complete sequence of both proteins, we have determined that the main linear antigenic determinants are located in the amino-terminal domains of these histones. The humoral response against histones H2B and H4 induced during canine leishmaniasis was found to be specific for Leishmania histones, since no cross-reactivity of the VCL sera with mammal histones was observed. Also, a comparative study of the prevalence of antibodies among VCL sera against the four core histones of L. infantum was performed. Although a large heterogeneity of the humoral responses against these proteins was found, histones H2A and H3 seem to be more prevalent immunogens than histones H2B and H4 during canine natural leishmaniasis. The origin of the anti-histone humoral response and its possible implications in the pathogenesis of Leishmania infection are discussed.read more
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Methods for diagnosis of canine leishmaniasis and immune response to infection
Carla Maia,Lenea Campino +1 more
TL;DR: The advantages and shortcomings of the available procedures for CanL diagnosis in the different phases, e.g. pre-patent and patent period of the infection and methods to determine the related immune response are reviewed.
Journal ArticleDOI
Parasite density and impaired biochemical/hematological status are associated with severe clinical aspects of canine visceral leishmaniasis
Alexandre Barbosa Reis,Alexandre Barbosa Reis,Olindo Assis Martins-Filho,Andréa Teixeira-Carvalho,Maria das Graças Carvalho,Wilson Mayrink,João Carlos França-Silva,Rodolfo Cordeiro Giunchetti,Rodolfo Cordeiro Giunchetti,Odair Genaro,Rodrigo Correa-Oliveira +10 more
TL;DR: It is observed that the progression of the disease from asymptomatic to symptomatic clinical form was accompanied by intense parasitism in the bone marrow, which led to the impaired biochemical/hematological status observed.
Journal ArticleDOI
Evolutionarily Conserved Proteins as Prominent Immunogens during Leishmania Infections
TL;DR: The elucidation of the pathways by which Leishmania antigens are processed and presented to effector cells from the host immune system will shed light on the immunopathology of leishmaniasis and help in the development of protective immunotherapies.
Journal ArticleDOI
Comparative evaluation of elisas based on ten recombinant or purified leishmania antigens for the serodiagnosis of mediterranean visceral leishmaniasis
Ibtissem Abdmouleh Maalej,Mehdi Chenik,Hechmi Louzir,Afif Ben Salah,Chokri Bahloul,F. Amri,Koussay Dellagi +6 more
TL;DR: Results show that several Leishmania antigens are suitable to design a diagnostic ELISA of MVL, however, recombinant proteins add little to the classic crude SLA, which still represents a very good and less costly alternative.
Journal ArticleDOI
Vaccination with a plasmid DNA cocktail encoding the nucleosomal histones of Leishmania confers protection against murine cutaneous leishmaniosis.
TL;DR: Data indicated that DNA vaccination with Leishmania histones genes results in a specific Th1-like response during L. major infection, and that both CD4(+) and CD8(+) T cells contribute to the resistance of vaccinated mice to cutaneous leishmaniasis.
References
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Journal Article
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Jorge Alvar,Carmen Cañavate,Beatriz Gutiérrez-Solar,Maribel Jiménez,Fernando Laguna,Rogelio López-Vélez,Ricardo Molina,Javier Moreno +7 more
TL;DR: Over 850 Leishmania-human immunodeficiency virus (HIV) coinfection cases have been recorded, the majority in Europe, where 7 to 17% of HIV-positive individuals with fever have amastigotes, suggesting that Leishmanniasis-infected individuals without symptoms will express symptoms of leishmaniasis if they become immunosuppressed.