Antioxidant proteins and reactive oxygen species are decreased in a murine epidermal side population with stem cell-like characteristics
Wanakee Carr,Rebecca E. Oberley-Deegan,Yuping Zhang,Christopher C. Oberley,Larry W. Oberley,Martine Dunnwald +5 more
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The data indicate ROS and antioxidant levels are decreased in stem-like EpSPs, which exhibited less mitochondrial area, fewer peroxisomes and produced lower levels of ROS than Non-SPs.Abstract:
Reactive oxygen species (ROS) and antioxidants are essential to maintain a redox balance within tissues and cells. Intracellular ROS regulate key cellular functions such as proliferation, differentiation and apoptosis through cellular signaling, and response to injury. The redox environment is particularly important for stem/progenitor cells, as their self-renewal and differentiation has been shown to be redox sensitive. However, not much is known about ROS and antioxidant protein function in freshly isolated keratinocytes, notably the different keratinocyte subpopulations. Immunostaining of neonatal cutaneous sections revealed that antioxidant enzymes [catalase, SOD2, gluthatione peroxidase-1 (GPx)] and ROS are localized predominantly to the epidermis. We isolated keratinocyte subpopulations and found lower levels of SOD2, catalase and GPx, as well as decreased SOD and catalase activity in an epidermal side population with stem cell-like characteristics (EpSPs) compared to more differentiated (Non-SP) keratinocytes. EpSPs also exhibited less mitochondrial area, fewer peroxisomes and produced lower levels of ROS than Non-SPs. Finally, EpSPs were more resistant to UV radiation than their progeny. Together, our data indicate ROS and antioxidant levels are decreased in stem-like EpSPs.read more
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Adipose-derived mesenchymal stem cell transplantation promotes adult neurogenesis in the brains of Alzheimer's disease mice.
TL;DR: It is proposed that adipose-derived mesenchymal stem cell transplantation enhances endogenous neurogenesis in both the subgranular and subventricular zones in APP/PS1 transgenic Alzheimer's disease mice, thereby facilitating functional recovery.
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Protective effects of various dosage of Curcumin against morphine induced apoptosis and oxidative stress in rat isolated hippocampus
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Protective effect of pre- and post-vitamin C treatments on UVB-irradiation-induced skin damage.
Saki Kawashima,Tomoko Funakoshi,Yasunori Sato,Norikatsu Saito,Hajime Ohsawa,Katsumi Kurita,Kisaburo Nagata,Masayuki Yoshida,Akihito Ishigami +8 more
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Antioxidant and Anti-Inflammatory Effects of Selected Natural Compounds Contained in a Dietary Supplement on Two Human Immortalized Keratinocyte Lines
Elena Fasano,Simona Serini,Nadia Mondella,Sonia Trombino,Leonardo Celleno,Paola Lanza,Achille Cittadini,Gabriella Calviello +7 more
TL;DR: The findings suggest that the combined formulation may have the potential to powerfully inhibit oxidative stress and inflammation at skin level.
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The effect of various morphine weaning regimens on the sequelae of opioid tolerance involving physical dependency, anxiety and hippocampus cell neurodegeneration in rats
Majid Motaghinejad,Seyed Morteza Karimian,Ozra Motaghinejad,Behnaz Shabab,Majid Asadighaleni,Sulail Fatima +5 more
TL;DR: In all treatment groups, GSH level, superoxide dismutase, glutathione peroxidase, and Bcl‐2 activity were significantly increased and SOD mimetic agent c diminished morphine effect on SOD activity.
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