Journal ArticleDOI
Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells.
James G. Rheinwatd,Howard Green +1 more
TLDR
Human diploid epidermis epidermal cells have been successfully grown in serial culture and it is possible to isolate keratinocyte clones free of viable fibroblasts, and human diploids keratinocytes appear to have a finite culture lifetime.About:
This article is published in Cell.The article was published on 1975-11-01. It has received 4114 citations till now. The article focuses on the topics: Plating efficiency & Cell culture.read more
Citations
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Cutaneous wound healing.
TL;DR: The primary goals of the treatment of wounds are rapid wound closure and a functional and aesthetically satisfactory scar.
Journal ArticleDOI
Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
TL;DR: The characteristics of the HaCaT cell line clearly document that spontaneous transformation of human adult keratinocytes can occur in vitro and is associated with sequential chromosomal alterations, though not obligatorily linked to major defects in differentiation.
Journal ArticleDOI
p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development
Annie Yang,Ronen Schweitzer,Deqin Sun,Mourad Kaghad,Nancy Walker,Roderick T. Bronson,Clifford J. Tabin,Arlene H. Sharpe,Daniel Caput,Christopher P. Crum,Frank McKeon +10 more
TL;DR: It is reported that mice homozygous for a disrupted p63 gene have major defects in their limb, craniofacial and epithelial development, and results indicate that p63 is critical for maintaining the progenitor-cell populations that are necessary to sustain epithelialDevelopment and morphogenesis.
Journal ArticleDOI
Wound repair and regeneration: Mechanisms, signaling, and translation
TL;DR: In this review, emerging concepts in tissue regeneration and repair are highlighted, and some perspectives on how to translate current knowledge into viable clinical approaches for treating patients with wound-healing pathologies are provided.
Journal ArticleDOI
Modeling Development and Disease with Organoids
TL;DR: 3D culture technology allow embryonic and adult mammalian stem cells to exhibit their remarkable self-organizing properties, and the resulting organoids reflect key structural and functional properties of organs such as kidney, lung, gut, brain and retina, and hold promise to predict drug response in a personalized fashion.
References
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Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
The limited in vitro lifetime of human diploid cell strains
TL;DR: The survival curves obtained with human diploid cell strains are comparable to “multiple-hit” or “ multiple-target” curves obtain with other biological systems where an initial threshold dose is required before an exponential form of the curve is established.
Journal ArticleDOI
Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines
George J. Todaro,Howard Green +1 more
TL;DR: Disaggregated mouse embryo cells, grown in monolayers, underwent a progressive decline in growth rate upon successive transfer, the rapidity of the decline depending on the inoculation density, but nearly all cultures developed into established lines within 3 months of culture.
Journal ArticleDOI
Formation of a keratinizing epithelium in culture by a cloned cell line derived from a teratoma
James G. Rheinwald,Howard Green +1 more
TL;DR: From a transplantable mouse teratoma it has been possible to derive an established keratinizing cell line (XB) which grows well in cultures containing lethally irradiated 3T3 fibroblasts at the correct density.
Journal ArticleDOI
Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth
TL;DR: FGF, a polypeptide found in brain and pituitary, provokes the initiation of DNA synthesis by resting ST3 cells by stimulating DNA synthesis as effectively as serum when glucocorticoids are present.
Related Papers (5)
Three clonal types of keratinocyte with different capacities for multiplication
Yann Barrandon,Howard Green +1 more