Journal ArticleDOI
Aortic endothelial cells synthesize basic fibroblast growth factor which remains cell associated and platelet-derived growth factor-like protein which is secreted.
Israel Vlodavsky,Rafael Fridman,Robert Sullivan,Joachim Sasse,Michael Klagsbrun,Michael Klagsbrun +5 more
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TLDR
It is suggested that endothelial cell‐associated basic FGF acts in an autocrine fashion to stimulate endothelium cell proliferation in response to endothelialcell perturbation or injury.Abstract:
Cultured bovine aortic endothelial cells synthesize growth factors which markedly differ in the regulation of their storage and secretion. Endothelial cell lysates, but not conditioned medium, contain a growth factor activity that appears to be basic fibroblast growth factor (FGF) by the following criteria: (1) it elutes from heparin-Sepharose at 1.4–1.6 M NaCl; (2) it is mitogenic for bovine aortic and capillary endothelial cells; (3) it is heat sensitive but stable to dithiothreitol; (4) it has a molecular weight of about 18,000 daltons; and (5) it cross-reacts with antiserum directed against basic FGF. In contrast, endothelial cell conditioned medium, but not lysates, contains a growth factor activity that (1) elutes from heparin-Sepharose at 0.4 – 0.5 M NaCl; (2) is mitogenic for fibroblasts and vascular smooth muscle cells but not for capillary endothelial cells; (3) is heat stable and dithiothreitol sensitive; and (4) competes with platelet-derived growth factor (PDGF) for binding to fibroblasts. From these criteria, it appears that endothelial cells secrete into the medium growth factors some of which are PDGF-like, but secrete little if any basic FGF. It is suggested that endothelial cell-associated basic FGF acts in an autocrine fashion to stimulate endothelial cell proliferation in response to endothelial cell perturbation or injury. On the other hand, the endothelial cell-secreted growth factors which are smooth muscle cell but not endothelial cell mitogens might exert a paracrine function on neighboring cells of the vessel wall.read more
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Journal ArticleDOI
Regulators of angiogenesis.
TL;DR: The goal of this review is to describe those stimulators and inhibitors of angiogenesis that have been best-characterized.
Journal ArticleDOI
Autocrine activities of basic fibroblast growth factor: regulation of endothelial cell movement, plasminogen activator synthesis, and DNA synthesis.
Yasufumi Sato,Daniel B. Rifkin +1 more
TL;DR: It is found that the spontaneous migration of bovine aortic endothelial cells from the edge of a denuded area in a confluent monolayer is dependent upon the release of endogenous basic fibroblast growth factor (bFGF).
Journal ArticleDOI
Inhibition of Capillary Endothelial Cell Growth by Pericytes and Smooth Muscle Cells
A. Orlidge,Patricia A. D'Amore +1 more
TL;DR: The results suggest that pericytes and SMCs can modulate EC growth by a mechanism that requires contact or proximity and postulate that similar interactions may operate to modulate vascular growth in vivo.
Journal ArticleDOI
Neovascularization is associated with a switch to the export of bFGF in the multistep development of fibrosarcoma.
Jessica J. Kandel,Jessica J. Kandel,Ella Bossy-Wetzel,François Radvanyi,Michael Klagsbrun,Michael Klagsbrun,Judah Folkman,Judah Folkman,Judah Folkman,Douglas Hanahan +9 more
TL;DR: In this multistep tumorigenesis pathway of dermal fibrosarcomas, there appears to be a discrete switch to the angiogenic phenotype that correlates with the export of bFGF, a knownAngiogenic factor.
Journal ArticleDOI
Role of basic fibroblast growth factor in vascular lesion formation.
TL;DR: Results show that bFGF, which is synthesized by the arterial wall, could be a potent mitogen for SMCs in vivo and suggest that any release of endogenous bF GF may be capable of stimulating SMC proliferation, which may subsequently lead to intimal lesion formation.
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The biology of platelet-derived growth factor
TL;DR: The biology of platelet derived growth factor, it will really give you the good idea to be successful.
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Heparin affinity: purification of a tumor-derived capillary endothelial cell growth factor
Yuen W. Shing,Judah Folkman,Ryan J. Sullivan,C Butterfield,James A. H. Murray,Michael Klagsbrun +5 more
TL;DR: A tumor-derived growth factor that stimulates the proliferation of capillary endothelial cells has a very strong affinity for heparin, which makes it possible to purify the growth factor to a single-band preparation in a rapid two-step procedure.
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Growth factors in bone matrix. Isolation of multiple types by affinity chromatography on heparin-Sepharose
TL;DR: Bone exhibits the most complex spectrum of growth factor activities of any tissue yet described, and mechanisms for unmasking or release of BDGFs from the mineralized matrix resulting in local action on target cells are undoubtedly important for the development and maintenance of bone tissue.
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Long-term culture of capillary endothelial cells
TL;DR: This prolonged culture method allows the production of pure capillary endothelial cells that may prove useful for studies of tumor angiogenesis, metastatic mechanisms, and the role ofcapillary endothelium in other pathologic states.
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Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF.
Frederick Esch,Andrew Baird,Nicholas Ling,Naoto Ueno,Fred Hill,Luc Denoroy,Robert Klepper,Denis Gospodarowicz,Peter Bohlen,Roger Guillemin +9 more
TL;DR: The two major mitogenic polypeptides for endothelial cells have been purified to homogeneity and the available protein sequence data demonstrate the existence of significant structural homology between the two polyPEptides.