Assaying genome-wide recombination and centromere functions with Arabidopsis tetrads.
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By determining the number and distribution of crossovers in individual meiotic cells, this work demonstrated a surprisingly precise regulation of crossover number in each meiosis, considerably reduced recombination along chromosomes carrying ribosomal DNA arrays, and an inversely proportional relationship between recombination frequencies and chromosome size.Abstract:
During meiosis, crossover events generate new allelic combinations, yet the abundance of these genetic exchanges in individual cells has not been measured previously on a genomic level. To perform a genome-wide analysis of recombination, we monitored the assortment of genetic markers in meiotic tetrads from Arabidopsis. By determining the number and distribution of crossovers in individual meiotic cells, we demonstrated (i) surprisingly precise regulation of crossover number in each meiosis, (ii) considerably reduced recombination along chromosomes carrying ribosomal DNA arrays, and (iii) an inversely proportional relationship between recombination frequencies and chromosome size. This use of tetrad analysis also achieved precise mapping of all five Arabidopsis centromeres, localizing centromere functions in the intact chromosomes of a higher eukaryote.read more
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Book ChapterDOI
Arabidopsis Genome Initiative
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References
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A procedure for mapping Arabidopsis mutations using co‐dominant ecotype‐specific PCR‐based markers
TL;DR: A set of mapping markers have been designed for Arabidopsis thaliana that correspond to DNA fragments amplified by the polymerase chain reaction (PCR) that can be unambiguously mapped to one of the 10Arabidopsis chromosome arms in a single cross using a limited number of F2 progeny.
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Assignment of 30 Microsatellite Loci to the Linkage Map of Arabidopsis
Callum J. Bell,Joseph R. Ecker +1 more
TL;DR: After the presence of microsatellites in Arabidopsis and their utility for genetic mapping had been demonstrated, systematic screening for (CA)n and (GA)n sequences was carried out on marker-selected plasmid libraries and a small-insert genomic library.
Journal ArticleDOI
Levels of naturally occurring DNA polymorphism correlate with recombination rates in D. melanogaster
TL;DR: In this paper, the authors show that the amount of nucleotide diversity in natural populations of D. melanogaster is positively correlated with the regional rate of recombination and suggest that the correlation may result from genetic hitch-hiking associated with the fixation of advantageous mutants.
Journal ArticleDOI
Recombinant inbred lines for mapping RFLP and phenotypic markers in Arabidopsis thaliana
Clare Lister,Caroline Dean +1 more
TL;DR: Recombinant inbred lines of Arabidopsis thaliana (Arabidopsis) have been generated from a cross between the ecotypes Landsberg erecta and Columbia, providing the material to map new probes or phenotypic traits polymorphic between Landsberg erection and Columbia.
Journal ArticleDOI
The genetic control of meiosis.
Bruce S. Baker,Adelaide T. C. Carpenter,Michael S. Esposito,Rochelle Easton Esposito,L. Sandler +4 more
TL;DR: This work has shown that mutants with altered meiotic exchange with defective mating-type control of sporulation have an important effect on fertility and this work aims to clarify these effects.