Q2. What contributions have the authors mentioned in the paper "Assessment of genetic diversity among pakistani wheat (triticum aestivum l.) advanced breeding lines using rapd and sds-page" ?
Genetic diversity was assessed among 32 advanced wheat breeding lines included in the National Uniform Wheat Yield Trials ( 2006-07 ) of Pakistan using molecular ( DNA ) and biochemical ( SDS-PAGE ) markers this paper.
Q3. What future works have the authors mentioned in the paper "Assessment of genetic diversity among pakistani wheat (triticum aestivum l.) advanced breeding lines using rapd and sds-page" ?
In the future, varieties with broader genetic base should be approved to overcome the reduced crop production.
Q4. What could be the reasons for the high polymorphism obtained for SDS-PAGE?
The possible reasons for the high polymorphism obtained for SDS-PAGE in the present study could be due to assessment of genetic diversity based on the total seed protein as well as the DNA marker system used in the present study.
Q5. What is the reason for the higher diversity of wheat varieties?
Phenotypic selection of lines on the basis of few morphological characters may result in approval of cultivars with lesser variability because of the influence of environment on growth and development.
Q6. What was the method used to construct a dendrogram?
The data were subsequently used to construct a dendrogram using the unweighted pair group method of arithmetic averages (UPGMA) (Sneath and Sokal, 1973) employing sequential, agglomerative hierarchic and non-overlapping clustering (SAHN).
Q7. What is the significance of the study?
This coupled with the fact that the HMW-GS showed greater polymorphism in the studied material, suggests that seed storage protein profiles could be useful markers in the studies of genetic diversity and classification of adapted cultivars, thereby improving the efficiency of wheat breeding programs in Pakistan.
Q8. How many uls were prepared for RAPD analysis?
Reaction mixture of 20 ul was prepared containing 1x PCR buffer with (NH4)2SO4, 3mM MgCl2, 0.2mM dNTPs mix, 20 pmol RAPD primer, one unit of Taq DNA Polymerase (Fermentas, life sciences) and DNA template of 10 ng.
Q9. Why did Mukhtar et al. (2002) observe 61% polymorphis?
Another possible reason of low polymorphism can be that RAPD primers may amplify same size fragments from different genomic regions, particularly in wheat due to its hexaploid genome.
Q10. How many subunits of size were found in the two lines?
The results from comparison with standard molecular weight revealed that lines N2 and RF7 contained 17 subunits of size in the range described above followed by the N9, N7, N16, and RF6 each contained 15 subunits.
Q11. What was the software used for the computations?
All the computations were carried out using the software NTSYSpc (Numerical Taxonomy and Multivariate Analysis System), version 2.1 (Rohlf, 2000).
Q12. What is the main determinant of end-use quality?
Fufa et al. (2005) reported that seed storage protein was the major determinant of end-use quality (a highly selected trait), and that genetic diversity estimates based on seed storage protein were, therefore, lowest.
Q13. How many bands were generated by SDS-PAGE?
Size of the protein bands generated by SDS-PAGE (measured by protein standard marker ranging in molecular weight from 10-220KDa) ranged from 29-120KDa.
Q14. What was the correlation between RAPD and SDS-PAGE analysis?
RAPD similarity coefficients ranged from 0.75 to 0.94 with 61% polymorphism whereas similarity coefficients showed a wider range (0.47-1.0) in case of SDS-PAGE analysis with 73.6% polymorphism.