Journal ArticleDOI
Bacillus thuringiensis var. israelensis δ-endotoxin: Nucleotide sequence and characterization of the transcripts in Bacillus thuringiensis and Escherichia coli☆
E.S. Ward,David J. Ellar +1 more
TLDR
Interestingly, the highest level of delta-endotoxin mRNA is seen during mid-exponential growth of E. coli and the level appears to decrease as the cells enter the stationary phase of growth, consistent with a postulated cytolytic mechanism of action for the toxin.About:
This article is published in Journal of Molecular Biology.The article was published on 1986-09-05. It has received 92 citations till now. The article focuses on the topics: Bacillus thuringiensis israelensis & Bacillus thuringiensis.read more
Citations
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Journal ArticleDOI
Insecticidal crystal proteins of Bacillus thuringiensis.
Herman Höfte,H R Whiteley +1 more
TL;DR: In this article, a classification for crystal protein genes of Bacillus thuringiensis is presented, based on the insecticidal spectra and the amino acid sequences of the encoded proteins.
Journal ArticleDOI
Revision of the Nomenclature for the Bacillus thuringiensis Pesticidal Crystal Proteins
Neil Crickmore,Daniel R. Zeigler,Jerald S. Feitelson,E. Schnepf,J. Van Rie,Didier Lereclus,J. Baum,Donald H. Dean +7 more
TL;DR: A new nomenclature, based on hierarchical clustering using amino acid sequence identity, is proposed, consisting of 133 crystal proteins comprising 24 primary ranks are systematically arranged.
Journal ArticleDOI
Mechanisms for the modulation of membrane bilayer properties by amphipathic helical peptides.
TL;DR: Different amphipathic helical peptides can be membrane stabilizing or be lytic to membranes, depending on the structural motif of the helix, which in turn determines the nature of its association with membranes.
Journal ArticleDOI
Mosquitocidal toxins of bacilli and their genetic manipulation for effective biological control of mosquitoes.
TL;DR: The results of cloning mosquitocidal toxins in heterologous microorganisms show the potential of expanding the range of susceptible mosquito species by combining several toxins of different host specificity in one cell, and the potential for increasing potency by persisting at the larval feeding zone.
Journal ArticleDOI
Contribution of the individual components of the δ-endotoxin crystal to the mosquitocidal activity of Bacillus thuringiensis subsp. israelensis
TL;DR: The δ-endotoxin crystal of the mosquitocidal bacterium Bacillus thuringiensis subsp. israelensis contains four major 0-endoxins as discussed by the authors.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
A simple method for displaying the hydropathic character of a protein
Jack Kyte,Russell F. Doolittle +1 more
TL;DR: A computer program that progressively evaluates the hydrophilicity and hydrophobicity of a protein along its amino acid sequence has been devised and its simplicity and its graphic nature make it a very useful tool for the evaluation of protein structures.
Journal ArticleDOI
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
H C Birnboim,J Doly +1 more
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.
Arapid alkaline extraction procedure forscreening recombinant plasmid DNA
TL;DR: The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes, and achievesequate pH control without using a pH meter.
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Bacillus thuringiensis var israelensis crystal delta-endotoxin: effects on insect and mammalian cells in vitro and in vivo
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A bacterial spore demonstrating rapid larvicidal activity against Anopheles sergentii, Uranotaenia unguiculata, Culex univitattus, Aedes aegypti and Culex pipiens [Insect pests]
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