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Biodiversity of cultivable psychrotrophic marine bacteria isolated from Terra Nova Bay (Ross Sea, Antarctica)

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TLDR
A set of 146 Antarctic marine isolates from the Ross Sea was characterized by a combination of molecular techniques in order to determine the degree of inter- and intraspecific variability, revealing the existence of horizontal gene transfer between strains belonging to the same and to different species.
Abstract
A set of 146 Antarctic marine isolates from the Ross Sea was characterized by a combination of molecular techniques in order to determine the degree of inter- and intraspecific variability. Isolates were analyzed by amplified rDNA restriction analysis (ARDRA) using the tetrameric enzyme AluI, resulting in 52 different groups, corresponding to at least 52 different bacterial species, indicating a high degree of interspecific variability. The phylogenetic position of bacteria belonging to some ARDRA groups was obtained by sequencing of 16S rDNA. Random amplified polymorphic DNA (RAPD) analysis, carried out on the largest ARDRA groups, revealed a high intraspecific genetic variability, too. The analysis of plasmid content revealed the existence of horizontal gene transfer between strains belonging to the same and to different species. A comparison of the whole body of morphological, physiological and biochemical data was finally carried out.

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Antarctic climate change and the environment

TL;DR: The Southern Hemisphere climate system varies on timescales from orbital, through millennial to sub-annual, and is closely coupled to other parts of the global climate system as discussed by the authors.
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Vitamin B12 and iron colimitation of phytoplankton growth in the Ross Sea

TL;DR: Iron and vitamin B12 plus iron treatments caused reductions in the DMSP : Chl a ratio relative to the control and B12 treatments, consistent with the notion of an antioxidant function for DMSP.
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Cold-water marine natural products

TL;DR: Marine natural products isolated from organisms collected from cold-water habitats are described, with emphasis on bioactive compounds from tunicates, sponges, microbes, bryozoans, corals, algae, molluscs and echinoderms.
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Diversity and genomics of Antarctic marine micro-organisms

TL;DR: Insights from initial genomics studies on both cultivated organisms and genomes accessed through shotgun cloning of environmental samples suggest that there are many unique features of these organisms that facilitate survival in high-latitude, persistently cold environments.
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Pronounced summer to winter differences and higher wintertime richness in coastal Antarctic marine bacterioplankton.

TL;DR: This paper conducted a comparative study of marine bacterioplankton sampled at several time points over the annual cycle (12 occasions each) at sub-Antarctic Kerguelen Islands (KI) and Antarctic Peninsula (AP) coastal sites in order to establish a better understanding of the extent and nature of variation in diversity and community structure at these different latitudes.
References
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Journal ArticleDOI

DNA sequencing with chain-terminating inhibitors

TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
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DNA polymorphisms amplified by arbitrary primers are useful as genetic markers

TL;DR: A new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence is described, suggesting that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
Journal ArticleDOI

Fingerprinting genomes using PCR with arbitrary primers

TL;DR: The generality of the arbitrarily primed PCR method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa.
Book

Nucleic acid techniques in bacterial systematics

TL;DR: Isolation and purification of nucleic acids DNA reassociation experiments DNA-rRNA hybridization and methods DNA sequencing in bacterial systematics direct sequence analysis of small RNAs 16S/23S rRNA sequencing the polymerase chain reaction development and application of nucleics acid probes DNA fingerprinting from macromolecules to trees.
Journal ArticleDOI

The rdp (ribosomal database project)

TL;DR: The Ribosomal Database Project (RDP-II), previously described by Maidak et al. (2000), continued during the past year to add new rRNA sequences to the aligned data and to improve the analysis commands.
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