Journal ArticleDOI
Biologically inspired strategy for programmed assembly of viral building blocks with controlled dimensions.
TLDR
It is envisioned that the biologically inspired assembly strategy to design and construct viral building blocks of controlled dimensions could be employed to fabricate well-controlled nanoarchitectures and hybrid nanomaterials for a wide variety of applications including nanoelectronics and nanocatalysis.Abstract:
Facile fabrication of building blocks with precisely controlled dimensions is imperative in the development of functional devices and materials. We demonstrate the assembly of nanoscale viral building blocks of controlled lengths using a biologically motivated strategy. To achieve this we exploit the simple self-assembly mechanism of Tobacco mosaic virus (TMV), whose length is solely governed by the length of its genomic mRNA. We synthesize viral mRNA of desired lengths using simple molecular biology techniques, and in vitro assemble the mRNA with viral coat proteins to yield viral building blocks of controlled lengths. The results indicate that the assembly of the viral building blocks is consistent and reproducible, and can be readily extended to assemble building blocks with genetically modified coat proteins (TMV1cys). Additionally, we confirm the potential utility of the TMV1cys viral building blocks with controlled dimensions via covalent and quantitative conjugation of fluorescent markers. We envision that our biologically inspired assembly strategy to design and construct viral building blocks of controlled dimensions could be employed to fabricate well-controlled nanoarchitectures and hybrid nanomaterials for a wide variety of applications including nanoelectronics and nanocatalysis.read more
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Journal ArticleDOI
TMV nanorods with programmed longitudinal domains of differently addressable coat proteins.
Fania Geiger,Fania Geiger,Fabian J. Eber,Sabine Eiben,Anna Mueller,Holger Jeske,Joachim P. Spatz,Joachim P. Spatz,Christina Wege +8 more
TL;DR: This study shows that tobacco mosaic virus and the RNA-guided self-assembly process of its coat protein (CP) can be used to establish new nanorod scaffolds that can be loaded not only with homogeneously distributed functionalities, but with distinct molecule species grouped and ordered along the longitudinal axis.
Journal ArticleDOI
Modified TMV Particles as Beneficial Scaffolds to Present Sensor Enzymes
Claudia Koch,Katrin Wabbel,Fabian J. Eber,Peter Krolla-Sidenstein,Carlos Azucena,Hartmut Gliemann,Sabine Eiben,Fania Geiger,Christina Wege +8 more
TL;DR: Tobacco mosaic virus can, thus, serve as a favorable multivalent nanoscale platform for the ordered presentation of bioactive proteins.
Journal ArticleDOI
Learning from nature – novel synthetic biology approaches for biomaterial design
TL;DR: The purpose of this review is to highlight the most recent advances in the use of molecular evolution in synthetic biology applications for biomaterial engineering, and to discuss some of the areas in which this approach may be successfully applied in the future.
Journal ArticleDOI
Novel roles for well-known players: from tobacco mosaic virus pests to enzymatically active assemblies.
Claudia Koch,Fabian J. Eber,Carlos Azucena,Alexander Förste,Stefan Walheim,Thomas Schimmel,Alexander M. Bittner,Holger Jeske,Hartmut Gliemann,Sabine Eiben,Fania Geiger,Christina Wege +11 more
TL;DR: This review gives the reader a ride through strikingly diverse achievements obtained with TMV-based particles, compares them to the progress with related viruses, and focuses on latest results revealing special advantages for enzyme-based biosensing formats, which might be of high interest for diagnostics employing 'systems-on-a-chip'.
Journal ArticleDOI
RNA-controlled assembly of tobacco mosaic virus-derived complex structures: from nanoboomerangs to tetrapods
TL;DR: The in vitro assembly of artificial nanotubular nucleoprotein shapes based on tobacco mosaic virus-(TMV-)-derived building blocks yielded different spatial organizations of viral coat protein subunits on genetically engineered RNA molecules, containing two or multiple TMV origins of assembly (OAs).
References
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Journal ArticleDOI
Nanoparticles, Proteins, and Nucleic Acids: Biotechnology Meets Materials Science
TL;DR: This review is focused on current approaches emerging at the intersection of materials research, nanosciences, and molecular biotechnology, which is closely associated with both the physical and chemical properties of organic and inorganic nanoparticles.
Journal ArticleDOI
Virus-Enabled Synthesis and Assembly of Nanowires for Lithium Ion Battery Electrodes
Ki Tae Nam,Dong Wan Kim,Pil J. Yoo,Chung Yi Chiang,Nonglak Meethong,Paula T. Hammond,Yet-Ming Chiang,Angela M. Belcher +7 more
TL;DR: Combining virus-templated synthesis at the peptide level and methods for controlling two-dimensional assembly of viruses on polyelectrolyte multilayers provides a systematic platform for integrating these nanomaterials to form thin, flexible lithium ion batteries.
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Synthesis and catalytic properties of metal nanoparticles: Size, shape, support, composition, and oxidation state effects
TL;DR: In this paper, the authors examine the role played by the particle structure and morphology (size and shape), its chemical composition and oxidation state, and the effect of the cluster support.
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Inorganic–Organic Nanotube Composites from Template Mineralization of Tobacco Mosaic Virus
TL;DR: This paper reports a new approach to the template-directed synthesis of inorganic±organic nanotubes using tobacco mosaic virus (TMV), and shows that TMV is a suitable template for reactions such as co-crystallization, oxidative hydrolysis, and sol-gel condensation.
Journal ArticleDOI
The tobacco mosaic virus particle: structure and assembly
TL;DR: A short account is given of the physical and chemical studies that have led to an understanding of the structure of the tobacco mosaic virus particle and how it is assembled from its constituent coat protein and RNA.