Brownian motion in biological membranes
TLDR
It is suggested that for a realistic situation translational diffusion should be about four times faster in relation to rotational diffusion than in the isotropic case.Abstract:
Brownian motion (diffusion) of particles in membranes occurs in a highly anisotropic environment. For such particles a translational mobility (independent of velocity) can be defined if the viscosity of the liquid embedding the membrane is taken into account. The results of a model calculation are presented. They suggest that for a realistic situation translational diffusion should be about four times faster in relation to rotational diffusion than in the isotropic case.read more
Citations
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Models for the specific adhesion of cells to cells
TL;DR: The force required to separate two cells is shown to be greater than the expected electrical forces between cells, and of the same order of magnitude as the forces required to pull gangliosides and perhaps some integral membrane proteins out of the cell membrane.
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Paradigm Shift of the Plasma Membrane Concept from the Two-Dimensional Continuum Fluid to the Partitioned Fluid: High-Speed Single-Molecule Tracking of Membrane Molecules
Akihiro Kusumi,Chieko Nakada,Ken Ritchie,K. Murase,Kenichi G. N. Suzuki,Hideji Murakoshi,Rinshi S. Kasai,Junko Kondo,Takahiro K. Fujiwara +8 more
TL;DR: The high-speed single-molecule tracking methods are described, and a new model of a partitioned fluid plasma membrane and the involvement of the actin-based membrane-skeleton "fences" and anchored-transmembrane protein "pickets" in the formation of compartment boundaries are critically reviewed.
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Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells.
TL;DR: In this article, the trajectories of E-cadherin, epidermal growth factor receptor, and transferrin receptor in the plasma membrane of a cultured mouse keratinocyte cell line were studied using both single particle tracking (SPT; nanovid microscopy) and fluorescence photobleaching recovery (FPR).
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Sphingolipid–Cholesterol Rafts Diffuse as Small Entities in the Plasma Membrane of Mammalian Cells
TL;DR: It is demonstrated that using protein constructs with identical ectodomains and different membrane regions and vice versa provides the viscous damping of the membrane domain in the lipid bilayer to probe the dynamics and size of lipid rafts in the membrane of living cells.
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Polymer-supported membranes as models of the cell surface
TL;DR: This work focuses on lipid-bilayer membranes supported on solid substrates, which are widely used as cell-surface models that connect biological and artificial materials and when these systems are coupled with advanced semiconductor technology.
References
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Rotational diffusion of rhodopsin in the visual receptor membrane.
TL;DR: Transient photodichroism in the frog retina reveals that rhodopsin has a relaxation time of 20 µs, and the site rhodopin occupies in the membrane must therefore be highly fluid.
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Lateral diffusion of rhodopsin in the photoreceptor membrane
Mu-ming Poo,Richard A. Cone +1 more
TL;DR: Rhodopsin undergoes rapid lateral diffusion in the disk membranes of isolated frog and mudpuppy rods, which indicates the disk membrane is highly fluid with a viscosity of ∼1P.
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Rotational and translational diffusion in membranes.
TL;DR: This review records evidence for diffusion of membrane components within membranes themselves and discusses numerical estimates of d iffusion in mem branes as well as less direct evidence for such diffusion.
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Lateral diffusion of rhodopsin in Necturus rods
Mu-ming Poo,Richard A. Cone +1 more
TL;DR: Fast lateral motion of rhodopsin within the disc membrane has been observed with micro-spectrophotometry and the rate of translational diffusion indicates that the disc membranes is highly fluid, having a viscosity consistent with that determined from rotational diffusion.