Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.
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The theoretical basis and some practical guidelines for simple, rigorous analysis of FPR experiments are presented and some model experiments on aqueous solutions of rhodamine 6G are described.About:
This article is published in Biophysical Journal.The article was published on 1976-09-01 and is currently open access. It has received 2594 citations till now. The article focuses on the topics: Fluorescence loss in photobleaching & Fluorescence recovery after photobleaching.read more
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The Immunological Synapse: A Molecular Machine Controlling T Cell Activation
Arash Grakoui,Shannon K. Bromley,Cenk Sumen,Mark M. Davis,Andrey S. Shaw,Paul M. Allen,Michael L. Dustin +6 more
TL;DR: Immunological synapse formation is now shown to be an active and dynamic mechanism that allows T cells to distinguish potential antigenic ligands and was a determinative event for T cell proliferation.
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Phase Separation by Low Complexity Domains Promotes Stress Granule Assembly and Drives Pathological Fibrillization
Amandine Molliex,Jamshid Temirov,Jihun Lee,Maura Coughlin,Anderson P. Kanagaraj,Hong Joo Kim,Tanja Mittag,J. Paul Taylor +7 more
TL;DR: It is demonstrated that the disease-related RBP hnRNPA1 undergoes liquid-liquid phase separation (LLPS) into protein-rich droplets mediated by a low complexity sequence domain (LCD), and suggested that LCD-mediated LLPS contributes to the assembly of stress granules and their liquid properties.
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Anomalous diffusion models and their properties: non-stationarity, non-ergodicity, and ageing at the centenary of single particle tracking.
TL;DR: This Perspective is intended as a guidebook for both experimentalists and theorists working on systems, which exhibit anomalous diffusion, and pays special attention to the ergodicity breaking parameters for the different anomalous stochastic processes.
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Diffusion in brain extracellular space.
Eva Syková,Charles Nicholson +1 more
TL;DR: Experimental studies with the real-time iontophoresis method employing the cation tetramethylammonium in normal brain tissue improve the conception of ECS structure and the roles of glia and extracellular matrix in modulating the ECS microenvironment.
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Studying protein dynamics in living cells.
TL;DR: Live cell imaging, in combination with photobleaching, energy transfer or fluorescence correlation spectroscopy are providing unprecedented insights into the movement of proteins and their interactions with cellular components.
References
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Book
Conduction of Heat in Solids
H. S. Carslaw,John Conrad Jaeger +1 more
TL;DR: In this paper, a classic account describes the known exact solutions of problems of heat flow, with detailed discussion of all the most important boundary value problems, including boundary value maximization.
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Fluorescence correlation spectroscopy. I. Conceptual basis and theory
Elliot L. Elson,Douglas Magde +1 more
TL;DR: In this article, a method for determining chemical kinetic constants and diffusion coefficients by measuring the rates of decay of spontaneous concentration fluctuations was proposed, which is based on the principle that the rate of spontaneous microscopic fluctuations are determined by the same phenomenological rate coefficients as those of macroscopic departures from equilibrium which result from external perturbations.
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Fluorescence Correlation Spectroscopy. II. An Experimental Realization
TL;DR: This paper describes the first experimental application of fluorescence correlation spectroscopy, a new method for determining chemical kinetic constants and diffusion coefficients by observing the time behaviour of the tiny concentration fluctuations which occur spontaneously in the reaction system even when it is in equilibrium.
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Lateral diffusion of rhodopsin in the photoreceptor membrane
Mu-ming Poo,Richard A. Cone +1 more
TL;DR: Rhodopsin undergoes rapid lateral diffusion in the disk membranes of isolated frog and mudpuppy rods, which indicates the disk membrane is highly fluid with a viscosity of ∼1P.