CD63-positive extracellular vesicles are potential diagnostic biomarkers of pancreatic ductal adenocarcinoma
Reads0
Chats0
TLDR
In this paper , the authors quantitatively measured the serum levels of extracellular vesicles (EVs) or platelet-derived EVs (CD41+- and CD61+-EVs), and evaluated their potential use as biomarkers of PDAC.Abstract:
Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest gastrointestinal cancers with a 5-year survival rate of less than 10%. Biomarkers for early PDAC detection are useful in treating patients with PDAC. Extracellular vesicles (EVs) are lipid-bound vesicles that are potential biomarkers of various diseases such as PDAC. In this study, we quantitatively measured the serum levels of EVs (CD63+-EVs) or platelet-derived EVs (CD41+- and CD61+-EVs) and evaluated their potential use as biomarkers of PDAC.We measured the serum levels of CD63+-, CD41+-, CD61+-EVs using sandwich enzyme-linked immunosorbent assay based on Tim4 with specificity for phosphatidylserine on EVs in age- and sex-matched healthy controls (HCs, n = 39) and patients with PDAC (n = 39). We also examined the effect of tumor burden on the serum EV levels after surgical resection (n = 28). CA19-9, a clinical PDAC biomarker, was also measured for comparison.Serum levels of CD63+-EVs, CD41+-EVs, and CD61+-EVs were significantly increased in patients with PDAC compared to HCs. Receiver operating characteristic analysis revealed that CD63+-EVs exhibited the highest diagnostic performance to discriminate patients with PDAC from HCs (area under the curve (AUC): 0.846), which was comparable to CA19-9 (AUC: 0.842). CA19-9 showed lower AUC values in early stages (I-II, AUC: 0.814) than in late stages (III-IV, AUC: 0.883) PDAC. Conversely, CD63+-EVs, CD41+-EVs, and CD61+-EVs showed comparable AUCs between early- and late-stage PDAC. The combined use of CA19-9 and CD63+-EVs showed a higher diagnostic performance for early-stage PDAC (AUC: 0.903) than CA19-9. The serum levels of CD63+-EVs, CD41+-EVs, CD61+-EVs, and CA19-9 decreased significantly after surgical resection, demonstrating that EVs are increased in sera of patients depending on the tumor burden.The serum levels of CD63+-EVs and platelet-derived EVs (CD41+-EVs, CD61+-EVs) are increased in patients with PDAC than HCs. Since CD63+-EVs showed a high AUC to discriminate patients with PDAC from HCs; they might be useful as potential biomarkers for PDAC. read more
Citations
More filters
Journal ArticleDOI
Cancer-derived small extracellular vesicles: emerging biomarkers and therapies for pancreatic ductal adenocarcinoma diagnosis/prognosis and treatment
TL;DR: This review provides a comprehensive overview of the most recent scientific and clinical research on the discovery and involvement of key molecules in cancer-derived s EVs for PDAC diagnosis/prognosis and strategies using cancer- derived sEVs forPDAC treatment.
Journal ArticleDOI
Status quo of Extracellular Vesicle isolation and detection methods for clinical utility.
TL;DR: Extracellular vesicles (EVs) are nano-sized particles that hold tremendous potential in the clinical space, as their biomolecular profiles hold a key to noninvasive liquid biopsy for cancer diagnosis and prognosis as mentioned in this paper .
Journal ArticleDOI
Small extracellular vesicles have distinct CD81 and CD9 tetraspanin expression profiles in plasma from rheumatoid arthritis patients.
TL;DR: In this paper , the authors characterized the CD63, CD81 and CD9 tetraspanin profiles in the membrane of single extracellular vesicles (EVs) from treatment-naïve rheumatoid arthritis patients and assessed potential discrepancies between methotrexate (MTX) responder groups.
Journal ArticleDOI
The Role of Exosomes in Pancreatic Ductal Adenocarcinoma Progression and Their Potential as Biomarkers
TL;DR: In this paper , the authors shed light on the involvement of exosomes and their cargos in pancreatic ductal adenocarcinoma (PDAC) progression and their feasibility as diagnostic and prognostic biomarkers.
Journal ArticleDOI
The Convergence of FTIR and EVs: Emergence Strategy for Non-Invasive Cancer Markers Discovery
TL;DR: In this article , the authors present a review of FTIR-based biomarker identification in extracellular vesicles (EVs) and the rationale of the integration of a computationally assisted approach, along with the challenges of clinical translation.
References
More filters
Journal ArticleDOI
Investigation of the freely available easy-to-use software 'EZR' for medical statistics.
TL;DR: EZR enables the application of statistical functions that are frequently used in clinical studies, such as survival analyses, including competing risk analyses and the use of time-dependent covariates, receiver operating characteristics analyses, meta-analyses, sample size calculation and so on, by point-and-click access.
Journal ArticleDOI
Albumin-Bilirubin (ALBI) Grade as Part of the Evidence-Based Clinical Practice Guideline for HCC of the Japan Society of Hepatology: A Comparison with the Liver Damage and Child-Pugh Classifications
Atsushi Hiraoka,Takashi Kumada,Masatoshi Kudo,Masashi Hirooka,Kunihiko Tsuji,Ei Itobayashi,Kazuya Kariyama,Toru Ishikawa,Kazuto Tajiri,Hironori Ochi,Toshifumi Tada,Hidenori Toyoda,Kazuhiro Nouso,Kouji Joko,Hideki Kawasaki,Yoichi Hiasa,Kojiro Michitaka +16 more
TL;DR: ALBI grade is a useful and easy classification system for assessment of hepatic function for therapeutic decision making and prognosis based on ALBI grade/ALBI-T score was better than that based on liver damage/modified JIS score and Child-Pugh/JIS score.
Journal ArticleDOI
Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes.
Joanna Kowal,Guillaume Arras,Marina Colombo,Mabel Jouve,Jakob Paul Morath,Bjarke Primdal-Bengtson,Florent Dingli,Damarys Loew,Mercedes Tkach,Clotilde Théry +9 more
TL;DR: This work demonstrates the presence of exosomal and nonexosomal subpopulations within small EVs, and proposes their differential separation by immuno-isolation using either CD63, CD81, or CD9, and provides guidelines to define subtypes of EVs for future functional studies.
Journal ArticleDOI
Glypican-1 identifies cancer exosomes and detects early pancreatic cancer
Sonia A. Melo,Linda B. Luecke,Christoph Kahlert,Agustín F. Fernández,Seth T. Gammon,Judith Kaye,Valerie S. LeBleu,Elizabeth A. Mittendorf,Juergen Weitz,Nuh N. Rahbari,Christoph Reissfelder,Christian Pilarsky,Mario F. Fraga,David Piwnica-Worms,Raghu Kalluri +14 more
TL;DR: GPC1+ crExos may serve as a potential non-invasive diagnostic and screening tool to detect early stages of pancreatic cancer to facilitate possible curative surgical therapy.
Journal ArticleDOI
Extracellular Vesicles: Unique Intercellular Delivery Vehicles.
TL;DR: This review focuses on recent findings and knowledge gaps in the area of EV biogenesis, release, and uptake and highlights examples whereby EV cargoes control basic cellular functions, including motility and polarization, immune responses, and development, and contribute to diseases such as cancer and neurodegeneration.