Journal ArticleDOI
Cell Cycle-Related Changes in Repopulating Capacity of Human Mobilized Peripheral Blood CD34+ Cells in Non-Obese Diabetic/Severe Combined Immune-Deficient Mice
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TLDR
The use of cell cycle fractionation to separate human mobilized peripheral blood CD34(+) cells capable of repopulating the bone marrow (BM) of non-obese diabetic/severe combined immune-deficient (NOD/SCID) mice is reported here and demonstrates that G0-G1 progression in vitro is associated with a decrease in engraftment capacity.Citations
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Mesenchymal stem cells
TL;DR: This work will review the information dealing with the biology of mesenchymal progenitors as it has been revealed mainly by ex vivo studies performed with bone marrow-derived cells.
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Primary murine MSC show highly efficient homing to the bone marrow but lose homing ability following culture.
TL;DR: It is suggested that in vitro propagation of BM-derived MSC dramatically decreases their homing to BM and spleen, as well as the seeding fraction in the BM was reduced and after transplantation of 48 h cultured primary MSC no CFU-F were detected in the lymphohematopoietic organs.
Journal ArticleDOI
Modulation of Hematopoietic Stem Cell Homing and Engraftment by CD26
TL;DR: The results suggest that hematopoietic stem cell engraftment is not absolute, as previously suggested, and indicate that improvement of bone marrow transplant efficiency may be possible in the clinic.
Journal ArticleDOI
High-level transduction and gene expression in hematopoietic repopulating cells using a human imunodeficiency virus type 1-based lentiviral vector containing an internal spleen focus forming virus promoter
Christophe Demaison,Kathryn L. Parsley,Gaby Brouns,Michaela Scherr,Karin Battmer,Christine Kinnon,Manuel Grez,Adrian J. Thrasher +7 more
TL;DR: A vesicular stomatitis virus G envelope protein (VSV-G)-pseudotyped human immunodeficiency virus type 1 (HIV-1) lentiviral-based vector system is used to transduce cord blood (CB) CD34+ cells over a limited time period and significant gene marking was observed in engrafted human lymphoid, myeloid, and progenitor cells in all transplanted Severe Combined Immunodeficient mice.
Journal ArticleDOI
Isolation of a highly quiescent subpopulation of primitive leukemic cells in chronic myeloid leukemia.
TL;DR: These findings provide the first direct and definitive evidence of a deeply but reversibly quiescent subpopulation of leukemic cells in patients with CML with both in vitro and in vivo stem cell properties.
References
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Journal ArticleDOI
Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection.
TL;DR: A replication-defective retrovirus vector is used to compare the efficiency of gene transfer in stationary and replicating rat embryo fibroblasts and, in agreement with previous results, gene transfer was inhibited 100-fold in stationary versus replicating cells.
Journal ArticleDOI
Differentiation and Proliferation of Hematopoietic Stem Cells
TL;DR: This simple model fits the understanding of the interactions of growth factors with hematopoietic progenitors and risks oversimplification of a very complex process.
Journal Article
Limiting dilution assays for the determination of immunocompetent cell frequencies. I. Data analysis.
TL;DR: The method presented here provides a simple and rapid procedure for the valid determination of immunocompetent cell frequencies and demonstrates the importance of using proper data analysis methods.
Journal Article
Multiple defects in innate and adaptive immunologic function in NOD/LtSz-scid mice.
Leonard D. Shultz,Peter A. Schweitzer,Sherri W. Christianson,Bruce Gott,Isabelle B. Schweitzer,B Tennent,S D McKenna,L Mobraaten,Thiruchandurai V. Rajan,Dale L. Greiner +9 more
TL;DR: The multiple defects in innate and adaptive immunity unique to the NOD/LtSz-scid/scid mouse provide an excellent in vivo environment for reconstitution with human hematopoietic cells.
Journal ArticleDOI
Identification of primitive human hematopoietic cells capable of repopulating NOD/SCID mouse bone marrow: Implications for gene therapy
Andre Larochelle,Josef Vormoor,Josef Vormoor,Helmut Hanenberg,Jean C.Y. Wang,Mickie Bhatia,Tsvee Lapidot,Tsvee Lapidot,Thomas Moritz,Barbara Murdoch,Xiang Li Xiao,Ikunoshin Kato,David R. Williams,John E. Dick +13 more
TL;DR: A novel human hematopoietic cell, the SCID–repopulating cell (SRC), a cell more primitive than most LTC–ICs and CFCs, that is capable of multilineage repopulation of the bone marrow of nonobese diabetic mice with severe combined immunodeficiency disease (NOD/SCID mice).